{"id":"d980dd80-355f-5522-a517-18beba15b9ce","stable_key":"46d15d9e-d3b5-544d-ba01-b785aa3e4f42:thiamine-def-cardiac-contraction-cessation","predicate":"withdrawal-reduces","statement":"Spontaneous contractions stopped after ten to eleven days of thiamine withdrawal, as degeneration began.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"24c4b250-1dd0-5d0f-b39d-126d7a04cc44","mechanism_event_label":"Prolonged deprivation eventually stopped the cultured heart cells beating.","subject":{"id":"36b7c3eb-068b-500f-af1c-078829df1ecc","slug":"thiamine","display_name":"Thiamine (vitamin B1)","entity_type_key":"small_molecule"},"object":{"id":"4e4ae958-f9ac-535e-975e-46ee088f2942","slug":"cardiomyocyte-spontaneous-contraction","display_name":"Cardiomyocyte spontaneous contraction","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"24c4b250-1dd0-5d0f-b39d-126d7a04cc44","stable_key":"46d15d9e-d3b5-544d-ba01-b785aa3e4f42:thiamine-def-cardiac-contraction-cessation-event","event_type":"biochemical_relationship","label":"Prolonged deprivation eventually stopped the cultured heart cells beating.","description":"Spontaneous contractions stopped after ten to eleven days of thiamine withdrawal, as degeneration began.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"36b7c3eb-068b-500f-af1c-078829df1ecc","slug":"thiamine","display_name":"Thiamine (vitamin B1)","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"4e4ae958-f9ac-535e-975e-46ee088f2942","slug":"cardiomyocyte-spontaneous-contraction","display_name":"Cardiomyocyte spontaneous contraction","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_location","value_text":"Abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_span","value_text":"spontaneous contractions ceased","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Newborn-rat cardiac cells in culture; thiamine omitted from growth medium without an added thiamine antagonist; time course and readdition experiments.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Thiamine omitted from rat cardiac-cell growth medium","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"This endpoint is distinct from human ejection fraction or arrhythmia risk; ATP causality was not isolated by this time course.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Thiamine research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"thiamine","display_name":"Thiamine (vitamin B1)","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Rattus norvegicus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Prolonged deprivation eventually stopped the cultured heart cells beating.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[zangen-1997-cardiac-culture] Thiamine deficiency in cardiac cells in culture (1997). https://pubmed.ncbi.nlm.nih.gov/9337073/ DOI: 10.1016/S0006-2952(97)00178-0","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Cultured cardiac cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"48bd51bc-5bd9-5c4e-8e47-c7ed242dd78b","evidence_kind":"source_excerpt","locator":"Lines 1367-1379","start_line":1367,"end_line":1379,"excerpt":"### thiamine-def-cardiac-contraction-cessation\nSpontaneous contractions stopped after ten to eleven days of thiamine withdrawal, as degeneration began.\nCondition category: nutrient_deficiency\nnutrient_topic: Thiamine research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Prolonged deprivation eventually stopped the cultured heart cells beating.\norganism: Rattus norvegicus\ntissue_or_cell_type: Cultured cardiac cells\nexperimental_model: Newborn-rat cardiac cells in culture; thiamine omitted from growth medium without an added thiamine antagonist; time course and readdition experiments.\nlimitations: This endpoint is distinct from human ejection fraction or arrhythmia risk; ATP causality was not isolated by this time course.\nevidence_location: Abstract\nevidence_span: spontaneous contractions ceased\nexposure: Thiamine omitted from rat cardiac-cell growth medium\n[zangen-1997-cardiac-culture] Thiamine deficiency in cardiac cells in culture (1997). https://pubmed.ncbi.nlm.nih.gov/9337073/ DOI: 10.1016/S0006-2952(97)00178-0","model_system":"Newborn-rat cardiac cells in culture; thiamine omitted from growth medium without an added thiamine antagonist; time course and readdition experiments.","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [zangen-1997-cardiac-culture] Thiamine deficiency in cardiac cells in culture (1997). https://pubmed.ncbi.nlm.nih.gov/9337073/ DOI: 10.1016/S0006-2952(97)00178-0","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"158d2c03-ac8c-589f-8270-c468165ae346","stable_key":"import-46d15d9e-d3b5-544d-ba01-b785aa3e4f42","title":"Thiamine: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"f376512fb3310141315548015b387833e3af45146e73fb73cd02cee20e4ddb9c","revision_id":"53bc5eda-dec8-58cc-a56f-a9eb4ab036ef","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}