{"id":"d97b2f6c-71fe-59ff-a365-9a5568eccbb0","stable_key":"507ed066-fd6d-5722-9223-97b66302e7e3:glycine-gpr158-cortex","predicate":"mediates_tested","statement":"Bath glycine at 1 mM increased layer-II/III prefrontal neuronal excitability in control mice but not Gpr158-knockout mice under synaptic receptor blockade.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"eb9b94e7-80c1-5640-9862-5216e70c8039","mechanism_event_label":"This receptor can make selected neurons more excitable.","subject":{"id":"4c84160f-6db7-5bb4-aef1-a0c964306831","slug":"mouse-gpr158","display_name":"Mouse metabotropic glycine receptor / Gpr158","entity_type_key":"protein"},"object":{"id":"e621b550-b71c-5679-9b0e-4c052a043d15","slug":"mouse-prefrontal-glycine-excitability","display_name":"Glycine-evoked mouse prefrontal neuronal excitability","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"eb9b94e7-80c1-5640-9862-5216e70c8039","stable_key":"507ed066-fd6d-5722-9223-97b66302e7e3:glycine-gpr158-cortex-event","event_type":"observed_relationship","label":"This receptor can make selected neurons more excitable.","description":"Bath glycine at 1 mM increased layer-II/III prefrontal neuronal excitability in control mice but not Gpr158-knockout mice under synaptic receptor blockade.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"4c84160f-6db7-5bb4-aef1-a0c964306831","slug":"mouse-gpr158","display_name":"Mouse metabotropic glycine receptor / Gpr158","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"e621b550-b71c-5679-9b0e-4c052a043d15","slug":"mouse-prefrontal-glycine-excitability","display_name":"Glycine-evoked mouse prefrontal neuronal excitability","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"2b507258-430c-51fe-9fd2-e510c2c197a9","slug":"glycine","display_name":"Glycine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mouse acute slices, intrinsic-current measurements and knockout comparison.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"High bath exposure; absence of effect in layer V reinforces cell-type specificity.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Glycine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"glycine","display_name":"Glycine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"This receptor can make selected neurons more excitable.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Orphan receptor GPR158 serves as a metabotropic glycine receptor: mGlyR. · 2023 · https://pubmed.ncbi.nlm.nih.gov/36996198/ · DOI 10.1126/science.add7150","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"b8505fc8-b57b-534c-8557-571c279852b6","evidence_kind":"source_excerpt","locator":"Lines 250-256","start_line":250,"end_line":256,"excerpt":"## glycine-gpr158-cortex\nThis receptor can make selected neurons more excitable.\nBath glycine at 1 mM increased layer-II/III prefrontal neuronal excitability in control mice but not Gpr158-knockout mice under synaptic receptor blockade.\nModel: Mouse acute slices, intrinsic-current measurements and knockout comparison.\nLimitations: High bath exposure; absence of effect in layer V reinforces cell-type specificity.\nEvidence access: Primary full text\nOrphan receptor GPR158 serves as a metabotropic glycine receptor: mGlyR. · 2023 · https://pubmed.ncbi.nlm.nih.gov/36996198/ · DOI 10.1126/science.add7150","model_system":"Mouse acute slices, intrinsic-current measurements and knockout comparison.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"7b2859d8-e2bf-51e7-8e32-0715c10cbc49","stable_key":"import-507ed066-fd6d-5722-9223-97b66302e7e3","title":"Glycine: supply, one-carbon allocation, receptors and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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