{"id":"d97a2b18-8d98-57a7-864e-28729bb08a80","stable_key":"44eaeae5-557a-552b-8998-884f30462e2a:sulforaphane-hdac6-abundance","predicate":"reduced_protein_abundance_of","statement":"HDAC6 protein decreased early during treatment and HDAC6 transcript was suppressed after 48 hours.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"ec28a577-85eb-5ce3-9663-a509093f40cc","mechanism_event_label":"Protein abundance and catalytic activity must be recorded separately.","subject":{"id":"67e70215-70c4-546a-84b4-0819fac0b326","slug":"sulforaphane","display_name":"Sulforaphane / SFN, stereochemistry specified per study","entity_type_key":"small_molecule"},"object":{"id":"eb2fd44b-f489-56b7-814b-b18c0c402d63","slug":"hdac6","display_name":"Human histone deacetylase 6 / HDAC6","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"ec28a577-85eb-5ce3-9663-a509093f40cc","stable_key":"44eaeae5-557a-552b-8998-884f30462e2a:sulforaphane-hdac6-abundance-event","event_type":"biochemical_relationship","label":"Protein abundance and catalytic activity must be recorded separately.","description":"HDAC6 protein decreased early during treatment and HDAC6 transcript was suppressed after 48 hours.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"67e70215-70c4-546a-84b4-0819fac0b326","slug":"sulforaphane","display_name":"Sulforaphane / SFN, stereochemistry specified per study","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"eb2fd44b-f489-56b7-814b-b18c0c402d63","slug":"hdac6","display_name":"Human histone deacetylase 6 / HDAC6","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/sulforaphane-research/25307283.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"cc736d6c2f2dc6c4026bdad0a5835d69ed094775076a2c1606f47020e6783331\", \"start_char\": 0, \"end_char\": 1504, \"text_sha256\": \"cc736d6c2f2dc6c4026bdad0a5835d69ed094775076a2c1606f47020e6783331\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Matched cell-type and time-course comparison","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Sulforaphane exposure; serum and time-course comparisons","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Activity assays, protein abundance and substrate acetylation are distinct endpoints; mechanism of their divergence was not established. Erratum PMID 27271518 adds an omitted funding acknowledgment without revising these results.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Sulforaphane research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"sulforaphane","display_name":"Sulforaphane / SFN, stereochemistry specified per study","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human HaCaT keratinocytes and HCT116 colon cancer cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Protein abundance and catalytic activity must be recorded separately.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[sulforaphane-p25307283] The effect of sulforaphane on histone deacetylase activity in keratinocytes: Differences between in vitro and in vivo analyses. (2015). https://pubmed.ncbi.nlm.nih.gov/25307283/ DOI: 10.1002/mc.22224","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"HDAC activity, protein levels and target acetylation","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"4c482f64-ebab-5285-9c38-37a04baf4a46","evidence_kind":"source_excerpt","locator":"Lines 619-630","start_line":619,"end_line":630,"excerpt":"### sulforaphane-hdac6-abundance\nHDAC6 protein decreased early during treatment and HDAC6 transcript was suppressed after 48 hours.\nCondition category: normal\nnutrient_topic: Sulforaphane research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Protein abundance and catalytic activity must be recorded separately.\norganism: Human HaCaT keratinocytes and HCT116 colon cancer cells\ntissue_or_cell_type: HDAC activity, protein levels and target acetylation\nexperimental_model: Matched cell-type and time-course comparison\nlimitations: Activity assays, protein abundance and substrate acetylation are distinct endpoints; mechanism of their divergence was not established. Erratum PMID 27271518 adds an omitted funding acknowledgment without revising these results.\nexposure: Sulforaphane exposure; serum and time-course comparisons\nevidence_span: {\"source_cache\": \"artifacts/sulforaphane-research/25307283.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"cc736d6c2f2dc6c4026bdad0a5835d69ed094775076a2c1606f47020e6783331\", \"start_char\": 0, \"end_char\": 1504, \"text_sha256\": \"cc736d6c2f2dc6c4026bdad0a5835d69ed094775076a2c1606f47020e6783331\"}\n[sulforaphane-p25307283] The effect of sulforaphane on histone deacetylase activity in keratinocytes: Differences between in vitro and in vivo analyses. 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