{"id":"d9757908-281f-5585-87d4-c23ce043d2c8","stable_key":"475ea55a-65e9-51c3-a539-738a6a8f683f:mangiferin-sirt1-expression","predicate":"increases_expression","statement":"Norathyriol increased SIRT1 protein expression in HepG2 cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"dee545d6-47ea-57cd-a838-3efc6d55fd35","mechanism_event_label":"The metabolite altered an NAD-dependent regulatory pathway.","subject":{"id":"d0f7f42a-ac45-5144-9ea5-a7586896cc6e","slug":"norathyriol","display_name":"Norathyriol","entity_type_key":"small_molecule"},"object":{"id":"b8e1bfa9-c412-5f35-97af-cfc722123d27","slug":"sirt1","display_name":"SIRT1","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"dee545d6-47ea-57cd-a838-3efc6d55fd35","stable_key":"475ea55a-65e9-51c3-a539-738a6a8f683f:mangiferin-sirt1-expression-event","event_type":"biochemical_relationship","label":"The metabolite altered an NAD-dependent regulatory pathway.","description":"Norathyriol increased SIRT1 protein expression in HepG2 cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"d0f7f42a-ac45-5144-9ea5-a7586896cc6e","slug":"norathyriol","display_name":"Norathyriol","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"b8e1bfa9-c412-5f35-97af-cfc722123d27","slug":"sirt1","display_name":"SIRT1","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/mangiferin-research/29563875.fulltext.txt\", \"locator\": \"Primary full-text span; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"1a48a1f92d67cd91638969bb1ec3cb9f18cb3cb4e29c7e5f2bacf627dda76415\", \"start_char\": 35295, \"end_char\": 36269, \"text_sha256\": \"4b69cb6e7f09043eda3312b226bc03bbf8e0cbf25a4f6b51505c0aebed3b7d04\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"KK-Ay mouse liver experiments and mechanistic HepG2 studies","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Norathyriol in sodium-oleate lipid-loading model; micromolar range","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Phosphorylation and expression are scoped to experiments; no established human fatty-liver therapy or direct SIRT1 ligand claim.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Mangiferin research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"mangiferin","display_name":"Mangiferin","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Homo sapiens for HepG2 experiments; mouse findings separately scoped","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The metabolite altered an NAD-dependent regulatory pathway.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[mangiferin-p29563875] Mangiferin Improves Hepatic Lipid Metabolism Mainly Through Its Metabolite-Norathyriol by Modulating SIRT-1/AMPK/SREBP-1c Signaling. (2018). https://pubmed.ncbi.nlm.nih.gov/29563875/ DOI: 10.3389/fphar.2018.00201","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"HepG2 cells","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"b4416483-9ef2-540a-8755-5ae4da164aa1","evidence_kind":"source_excerpt","locator":"Lines 445-456","start_line":445,"end_line":456,"excerpt":"### mangiferin-sirt1-expression\nNorathyriol increased SIRT1 protein expression in HepG2 cells.\nCondition category: normal\nnutrient_topic: Mangiferin research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The metabolite altered an NAD-dependent regulatory pathway.\norganism: Homo sapiens for HepG2 experiments; mouse findings separately scoped\ntissue_or_cell_type: HepG2 cells\nexperimental_model: KK-Ay mouse liver experiments and mechanistic HepG2 studies\nlimitations: Phosphorylation and expression are scoped to experiments; no established human fatty-liver therapy or direct SIRT1 ligand claim.\nexposure: Norathyriol in sodium-oleate lipid-loading model; micromolar range\nevidence_span: {\"source_cache\": \"artifacts/mangiferin-research/29563875.fulltext.txt\", \"locator\": \"Primary full-text span; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"1a48a1f92d67cd91638969bb1ec3cb9f18cb3cb4e29c7e5f2bacf627dda76415\", \"start_char\": 35295, \"end_char\": 36269, \"text_sha256\": \"4b69cb6e7f09043eda3312b226bc03bbf8e0cbf25a4f6b51505c0aebed3b7d04\"}\n[mangiferin-p29563875] Mangiferin Improves Hepatic Lipid Metabolism Mainly Through Its Metabolite-Norathyriol by Modulating SIRT-1/AMPK/SREBP-1c Signaling. (2018). https://pubmed.ncbi.nlm.nih.gov/29563875/ DOI: 10.3389/fphar.2018.00201","model_system":"KK-Ay mouse liver experiments and mechanistic HepG2 studies","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [mangiferin-p29563875] Mangiferin Improves Hepatic Lipid Metabolism Mainly Through Its Metabolite-Norathyriol by Modulating SIRT-1/AMPK/SREBP-1c Signaling. (2018). https://pubmed.ncbi.nlm.nih.gov/29563875/ DOI: 10.3389/fphar.2018.00201","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"267f942a-695a-5b62-a858-f71c06321ff5","stable_key":"import-475ea55a-65e9-51c3-a539-738a6a8f683f","title":"Mangiferin: metabolism, signaling and nutrient connections (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. 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