{"id":"d95b69e3-61bd-5ac2-932b-5bff8d614e44","stable_key":"1ff3a29f-ef80-5bff-9300-30b98c60b90d:strontium-casr-independent-growth","predicate":"increases","statement":"Strontium increased replication in both wild-type and Casr-knockout mouse osteoblasts despite loss of the rapid ERK response in knockout cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"144910d2-185d-5443-9005-8ef20b63f93a","mechanism_event_label":"Losing one receptor branch did not remove every growth response.","subject":{"id":"97607549-3a37-544e-b6e6-266887f7c35f","slug":"strontium-ion","display_name":"Strontium ion / Sr2+","entity_type_key":"ion"},"object":{"id":"2418278c-8010-5a08-adf9-4b03a3639340","slug":"mouse-casr-knockout-osteoblast-replication","display_name":"Replication of Casr-knockout mouse osteoblasts","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"144910d2-185d-5443-9005-8ef20b63f93a","stable_key":"1ff3a29f-ef80-5bff-9300-30b98c60b90d:strontium-casr-independent-growth-event","event_type":"observed_relationship","label":"Losing one receptor branch did not remove every growth response.","description":"Strontium increased replication in both wild-type and Casr-knockout mouse osteoblasts despite loss of the rapid ERK response in knockout cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"97607549-3a37-544e-b6e6-266887f7c35f","slug":"strontium-ion","display_name":"Strontium ion / Sr2+","entity_type_key":"ion"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"2418278c-8010-5a08-adf9-4b03a3639340","slug":"mouse-casr-knockout-osteoblast-replication","display_name":"Replication of Casr-knockout mouse osteoblasts","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"2ca2864b-1ef0-54e4-8397-1dbe7207a0d2","slug":"strontium","display_name":"Strontium","entity_type_key":"nutrient_element"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"18071fe3-a86f-5dac-b440-1e2e59d22f24","slug":"mouse-casr","display_name":"Mouse calcium-sensing receptor / Casr","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract and accessible full-text Methods","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Same primary mouse-cell study and defined SrCl2/ranelate mixture.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Does not identify the alternative sensor or establish universal CaSR independence.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Strontium collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"strontium","display_name":"Strontium","entity_type_key":"nutrient_element"}},{"dimension":"plain_language","value_text":"Losing one receptor branch did not remove every growth response.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Calcium sensing receptor-dependent and receptor-independent activation of osteoblast replication and survival by strontium ranelate. · 2009 · https://pubmed.ncbi.nlm.nih.gov/20141614/ · DOI 10.1111/j.1582-4934.2009.00673.x","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"961de777-6384-5d97-84c3-313e19952050","evidence_kind":"source_excerpt","locator":"Lines 94-100","start_line":94,"end_line":100,"excerpt":"## strontium-casr-independent-growth\nLosing one receptor branch did not remove every growth response.\nStrontium increased replication in both wild-type and Casr-knockout mouse osteoblasts despite loss of the rapid ERK response in knockout cells.\nModel: Same primary mouse-cell study and defined SrCl2/ranelate mixture.\nLimitations: Does not identify the alternative sensor or establish universal CaSR independence.\nEvidence access: Primary abstract and accessible full-text Methods\nCalcium sensing receptor-dependent and receptor-independent activation of osteoblast replication and survival by strontium ranelate. · 2009 · https://pubmed.ncbi.nlm.nih.gov/20141614/ · DOI 10.1111/j.1582-4934.2009.00673.x","model_system":"Same primary mouse-cell study and defined SrCl2/ranelate mixture.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"43ef0fe7-eb6c-5346-8381-f7694733b4dc","stable_key":"import-1ff3a29f-ef80-5bff-9300-30b98c60b90d","title":"Strontium: calcium interactions, cellular mechanisms and mineralization (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"51221949263cdf8a22a79c32e3989599de1a37dc215700e0a076f436b40a1005","revision_id":"84f6df49-c456-5ad2-8214-cc69d5dc7722","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}