{"id":"d95a277c-813a-52b2-9a8c-8527fea3f5a7","stable_key":"e37461ea-ea5d-5e2c-8091-138305f6dd70:l-aspartate-eaat1-respiration","predicate":"supports_external_aspartate_rescue","statement":"SLC1A3 manipulation changed aspartate import and cancer-cell sensitivity to respiratory inhibitors.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"b650fb94-fc2f-519f-a8ee-1b982c51e84a","mechanism_event_label":"Transport capacity determines whether a cell can use the aspartate available outside it.","subject":{"id":"23bdf240-9ac3-57fc-8270-af878c463361","slug":"slc1a3","display_name":"Human excitatory amino acid transporter 1 / SLC1A3","entity_type_key":"protein"},"object":{"id":"9ad76d3d-5957-5829-8aed-b84db68ae357","slug":"human-cancer-etc-inhibitor-resistance","display_name":"Resistance to ETC-inhibitor growth suppression in human cancer cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"b650fb94-fc2f-519f-a8ee-1b982c51e84a","stable_key":"e37461ea-ea5d-5e2c-8091-138305f6dd70:l-aspartate-eaat1-respiration-event","event_type":"observed_relationship","label":"Transport capacity determines whether a cell can use the aspartate available outside it.","description":"SLC1A3 manipulation changed aspartate import and cancer-cell sensitivity to respiratory inhibitors.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"23bdf240-9ac3-57fc-8270-af878c463361","slug":"slc1a3","display_name":"Human excitatory amino acid transporter 1 / SLC1A3","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"9ad76d3d-5957-5829-8aed-b84db68ae357","slug":"human-cancer-etc-inhibitor-resistance","display_name":"Resistance to ETC-inhibitor growth suppression in human cancer cells","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"0a0923d3-72b7-5d6a-bf3a-5a7a3071a09b","slug":"l-aspartate","display_name":"L-Aspartate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"7da684a4-2641-5bc6-93ae-8c6aa384e487","slug":"glutamate","display_name":"L-Glutamate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human cancer-cell panels; SLC1A3 knockout/overexpression and inhibitor challenges.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"SLC1A3 also transports glutamate; effects are model-specific rather than an oral supplementation result. Correction record: The 2018 author correction added a missing competing-interests statement declaring no competing interests; no mechanism or data change was stated. PMID 30089842; DOI 10.1038/s41556-018-0184-2. https://pubmed.ncbi.nlm.nih.gov/30089842/","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Aspartate collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-aspartate","display_name":"L-Aspartate","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Transport capacity determines whether a cell can use the aspartate available outside it.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Aspartate is a limiting metabolite for cancer cell proliferation under hypoxia and in tumours. · 2018 · https://pubmed.ncbi.nlm.nih.gov/29941933/ · DOI 10.1038/s41556-018-0118-z","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"fc5e876c-53fe-52d1-85c7-db1157bbcec1","evidence_kind":"source_excerpt","locator":"Lines 74-80","start_line":74,"end_line":80,"excerpt":"## l-aspartate-eaat1-respiration\nTransport capacity determines whether a cell can use the aspartate available outside it.\nSLC1A3 manipulation changed aspartate import and cancer-cell sensitivity to respiratory inhibitors.\nModel: Human cancer-cell panels; SLC1A3 knockout/overexpression and inhibitor challenges.\nLimitations: SLC1A3 also transports glutamate; effects are model-specific rather than an oral supplementation result. Correction record: The 2018 author correction added a missing competing-interests statement declaring no competing interests; no mechanism or data change was stated. PMID 30089842; DOI 10.1038/s41556-018-0184-2. https://pubmed.ncbi.nlm.nih.gov/30089842/\nEvidence access: Primary full text\nAspartate is a limiting metabolite for cancer cell proliferation under hypoxia and in tumours. · 2018 · https://pubmed.ncbi.nlm.nih.gov/29941933/ · DOI 10.1038/s41556-018-0118-z","model_system":"Human cancer-cell panels; SLC1A3 knockout/overexpression and inhibitor challenges.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"67970dcb-34e0-5b0a-8c86-d8c3cc183444","stable_key":"import-e37461ea-ea5d-5e2c-8091-138305f6dd70","title":"L-Aspartate: redox transfer, nitrogen partitioning and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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