{"id":"d9516d2a-63ad-5083-bbdf-c72dcc0deecb","stable_key":"104835a3-b891-5dc4-a6bb-42ab7c6f96c4:butyrate-pth-microbial-depletion","predicate":"modulates","statement":"Antibiotic-depleted or germ-free female mice lost the bone-anabolic response to intermittent PTH; restoring physiological butyrate levels restored the response.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"efd4fd97-957f-53ef-b79e-9058b3d8d832","mechanism_event_label":"In this mouse model, the hormone response depended on a microbial metabolite.","subject":{"id":"e7428a7f-d35a-5406-9904-a88984814833","slug":"butyrate","display_name":"Butyrate","entity_type_key":"small_molecule"},"object":{"id":"878ea010-e9a2-5f23-8b59-1a0abc38977e","slug":"mouse-ipth-bone-anabolism","display_name":"Mouse bone-anabolic response to intermittent PTH","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"efd4fd97-957f-53ef-b79e-9058b3d8d832","stable_key":"104835a3-b891-5dc4-a6bb-42ab7c6f96c4:butyrate-pth-microbial-depletion-event","event_type":"observed_relationship","label":"In this mouse model, the hormone response depended on a microbial metabolite.","description":"Antibiotic-depleted or germ-free female mice lost the bone-anabolic response to intermittent PTH; 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unverified.","entity":null}],"evidence":[{"id":"35c9cef9-f44a-5149-8046-84daee38ecd4","evidence_kind":"source_excerpt","locator":"Lines 486-492","start_line":486,"end_line":492,"excerpt":"## butyrate-pth-microbial-depletion\nIn this mouse model, the hormone response depended on a microbial metabolite.\nAntibiotic-depleted or germ-free female mice lost the bone-anabolic response to intermittent PTH; restoring physiological butyrate levels restored the response.\nModel: Female mice; microbial depletion, daily subcutaneous human PTH(1-34) at 80 micrograms/kg for four weeks; 5 mM butyrate repletion in drinking water.\nLimitations: Not proof that human PTH treatment failure is usually butyrate deficiency, or that butyrate replaces PTH, calcium or vitamin D.\nEvidence access: Primary abstract and full-text intervention methods\nParathyroid hormone-dependent bone formation requires butyrate production by intestinal microbiota. · 2020 · https://pubmed.ncbi.nlm.nih.gov/31917685/ · DOI 10.1172/JCI133473","model_system":"Female mice; microbial depletion, daily subcutaneous human PTH(1-34) at 80 micrograms/kg for four weeks; 5 mM butyrate repletion in drinking water.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"33205bb8-dcbe-5d2c-afca-86075d7748d1","stable_key":"import-104835a3-b891-5dc4-a6bb-42ab7c6f96c4","title":"Butyrate: microbial production, fuel use, signaling and nutrient interactions (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. 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