{"id":"d9237a3a-36a7-5e9d-8688-f301095d5e92","stable_key":"5102beb6-9f61-500e-af10-9ac63649e0b7:k-deprivation-glud1","predicate":"deprivation-increases","statement":"Potassium deprivation increased renal glutamate dehydrogenase expression in the studied rats.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"18649d29-e4e5-5872-81f5-fb532c613387","mechanism_event_label":"A second ammonia-producing enzyme increased; this connects glutamate nitrogen to ammonium and its carbon skeleton to 2-oxoglutarate.","subject":{"id":"87435e1d-e1cd-57da-984b-203b2f5f29a4","slug":"potassium","display_name":"Potassium","entity_type_key":"nutrient_element"},"object":{"id":"7a2271b6-fae8-5b08-b6ae-df7a0f96919b","slug":"glud1","display_name":"GLUD1","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"18649d29-e4e5-5872-81f5-fb532c613387","stable_key":"5102beb6-9f61-500e-af10-9ac63649e0b7:k-deprivation-glud1-event","event_type":"biochemical_relationship","label":"A second ammonia-producing enzyme increased; 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unverified.","entity":null},{"dimension":"cross_nutrient","value_text":"Potassium deprivation changes glutamine nitrogen metabolism and sodium-coupled glutamine transport; these experiments did not manipulate dietary protein adequacy.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"endpoint","value_text":"Potassium deprivation increased renal glutamate dehydrogenase expression in the studied rats.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental-exposure","value_text":"Rats on control or potassium-deficient diets for up to 6 days; NH4Cl co-loading and NRK52E/LLC-PK1 cell experiments.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Rats on control or potassium-deficient diets for up to 6 days; NH4Cl co-loading and NRK52E/LLC-PK1 cell experiments.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Dietary deprivation precedes detectable hypokalemia here; protein expression does not independently establish transport flux or quantify net bicarbonate generation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Potassium research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"potassium","display_name":"Potassium","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Rattus norvegicus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"A second ammonia-producing enzyme increased; this connects glutamate nitrogen to ammonium and its carbon skeleton to 2-oxoglutarate.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[hossain-2011-k-ammoniagenesis] Cellular and molecular basis of increased ammoniagenesis in potassium deprivation (2011). https://journals.physiology.org/doi/full/10.1152/ajprenal.00010.2011 DOI: 10.1152/ajprenal.00010.2011","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"renal proximal tubule and whole-kidney excretion","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"1b831176-0ce2-5113-a720-463e38aadd3a","evidence_kind":"source_excerpt","locator":"Lines 1000-1012","start_line":1000,"end_line":1012,"excerpt":"### k-deprivation-glud1\nPotassium deprivation increased renal glutamate dehydrogenase expression in the studied rats.\nCondition category: nutrient_deficiency\nnutrient_topic: Potassium research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: A second ammonia-producing enzyme increased; this connects glutamate nitrogen to ammonium and its carbon skeleton to 2-oxoglutarate.\norganism: Rattus norvegicus\ntissue_or_cell_type: renal proximal tubule and whole-kidney excretion\nexperimental_model: Rats on control or potassium-deficient diets for up to 6 days; NH4Cl co-loading and NRK52E/LLC-PK1 cell experiments.\nlimitations: Dietary deprivation precedes detectable hypokalemia here; protein expression does not independently establish transport flux or quantify net bicarbonate generation.\ncross_nutrient: Potassium deprivation changes glutamine nitrogen metabolism and sodium-coupled glutamine transport; these experiments did not manipulate dietary protein adequacy.\nexperimental-exposure: Rats on control or potassium-deficient diets for up to 6 days; NH4Cl co-loading and NRK52E/LLC-PK1 cell experiments.\nendpoint: Potassium deprivation increased renal glutamate dehydrogenase expression in the studied rats.\n[hossain-2011-k-ammoniagenesis] Cellular and molecular basis of increased ammoniagenesis in potassium deprivation (2011). https://journals.physiology.org/doi/full/10.1152/ajprenal.00010.2011 DOI: 10.1152/ajprenal.00010.2011","model_system":"Rats on control or potassium-deficient diets for up to 6 days; NH4Cl co-loading and NRK52E/LLC-PK1 cell experiments.","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [hossain-2011-k-ammoniagenesis] Cellular and molecular basis of increased ammoniagenesis in potassium deprivation (2011). https://journals.physiology.org/doi/full/10.1152/ajprenal.00010.2011 DOI: 10.1152/ajprenal.00010.2011","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"241226ee-6b7d-5cc7-9781-e6beb8ee1982","stable_key":"import-5102beb6-9f61-500e-af10-9ac63649e0b7","title":"Potassium: cross-nutrient mechanisms and deficiency (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"a8f89f8d84b48c0f93048dfc45f7f57a67dd9a7249968a096a14aeb03d8b7a52","revision_id":"de4ec6f4-58a1-537b-8169-97b783898df1","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}