{"id":"d90da63d-178d-57ec-8841-04c0bbeecdab","stable_key":"f992b796-377d-53bf-a39b-7e5d41dbe194:b5-met-heart-acp-respiration","predicate":"deletion_reduces","statement":"Cardiac Ndufab1 deletion lowered oxygen consumption supported by complex I-, II-, or III-linked substrates, while complex IV-linked respiration remained unchanged.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"80c9d2d1-82b5-5f44-ba04-c3cce32f3a9c","mechanism_event_label":"The respiratory defect affected several upstream electron-transfer routes but spared the tested complex IV route.","subject":{"id":"2b43af69-e964-5a70-896a-3f424d79d2d9","slug":"mouse-ndufab1","display_name":"Mouse mitochondrial acyl carrier protein / Ndufab1","entity_type_key":"protein"},"object":{"id":"edbd60a3-32ad-5cdb-8569-2914b6a5b0c9","slug":"mitochondrial-adp-stimulated-oxygen-consumption","display_name":"ADP-stimulated mitochondrial oxygen consumption","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"80c9d2d1-82b5-5f44-ba04-c3cce32f3a9c","stable_key":"f992b796-377d-53bf-a39b-7e5d41dbe194:b5-met-heart-acp-respiration-event","event_type":"biochemical_relationship","label":"The respiratory defect affected several upstream electron-transfer routes but spared the tested complex IV route.","description":"Cardiac Ndufab1 deletion lowered oxygen consumption supported by complex I-, II-, or III-linked substrates, while complex IV-linked respiration remained unchanged.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"f67ac698-de2e-55f5-8975-305eaadbb751","slug":"respiratory-complex-i","display_name":"Mitochondrial respiratory complex I","entity_type_key":"protein_complex"},"role":"substrate_linked_route","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"4dc2faa3-f38c-5813-b7f2-fe19a0ab7bfa","slug":"respiratory-complex-ii","display_name":"Respiratory complex II","entity_type_key":"protein_complex"},"role":"substrate_linked_route","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"3b73f316-a551-517d-82b8-6af922d5a091","slug":"respiratory-complex-iii","display_name":"Mitochondrial respiratory complex III","entity_type_key":"protein_complex"},"role":"substrate_linked_route","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"2b43af69-e964-5a70-896a-3f424d79d2d9","slug":"mouse-ndufab1","display_name":"Mouse mitochondrial acyl carrier protein / Ndufab1","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"edbd60a3-32ad-5cdb-8569-2914b6a5b0c9","slug":"mitochondrial-adp-stimulated-oxygen-consumption","display_name":"ADP-stimulated mitochondrial oxygen consumption","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"cross_nutrient","value_text":"false","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_location","value_text":"Full text lines 47–49; Fig. 3A","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Cardiomyocyte-specific Ndufab1 knockout mice and isolated heart mitochondria","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Cardiac-specific genetic deletion; 6- and 16-week assembly assays, 6- and 10-week respiratory assays.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Isolated-mitochondria oxygen consumption does not measure nutrient absorption or human exercise capacity. This experiment does not establish a dietary pantothenate threshold or benefit from B5 supplementation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Pantothenic acid (vitamin B5) research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"pantothenate","display_name":"Pantothenate (vitamin B5)","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Mus musculus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The respiratory defect affected several upstream electron-transfer routes but spared the tested complex IV route.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[b5-met-ndufab2019] NDUFAB1 confers cardio-protection by enhancing mitochondrial bioenergetics through coordination of respiratory complex and supercomplex assembly. (2019). https://pubmed.ncbi.nlm.nih.gov/31366990/ DOI: 10.1038/s41422-019-0208-x","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Heart mitochondria","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"6f5ff59b-548f-57b2-84c1-89fc9ec9fe1e","evidence_kind":"source_excerpt","locator":"Lines 963-975","start_line":963,"end_line":975,"excerpt":"### b5-met-heart-acp-respiration\nCardiac Ndufab1 deletion lowered oxygen consumption supported by complex I-, II-, or III-linked substrates, while complex IV-linked respiration remained unchanged.\nCondition category: machinery_impairment\nnutrient_topic: Pantothenic acid (vitamin B5) research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The respiratory defect affected several upstream electron-transfer routes but spared the tested complex IV route.\norganism: Mus musculus\ntissue_or_cell_type: Heart mitochondria\nexperimental_model: Cardiomyocyte-specific Ndufab1 knockout mice and isolated heart mitochondria\nlimitations: Isolated-mitochondria oxygen consumption does not measure nutrient absorption or human exercise capacity. This experiment does not establish a dietary pantothenate threshold or benefit from B5 supplementation.\nexposure: Cardiac-specific genetic deletion; 6- and 16-week assembly assays, 6- and 10-week respiratory assays.\ncross_nutrient: false\nevidence_location: Full text lines 47–49; Fig. 3A\n[b5-met-ndufab2019] NDUFAB1 confers cardio-protection by enhancing mitochondrial bioenergetics through coordination of respiratory complex and supercomplex assembly. (2019). https://pubmed.ncbi.nlm.nih.gov/31366990/ DOI: 10.1038/s41422-019-0208-x","model_system":"Cardiomyocyte-specific Ndufab1 knockout mice and isolated heart mitochondria","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [b5-met-ndufab2019] NDUFAB1 confers cardio-protection by enhancing mitochondrial bioenergetics through coordination of respiratory complex and supercomplex assembly. (2019). https://pubmed.ncbi.nlm.nih.gov/31366990/ DOI: 10.1038/s41422-019-0208-x","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"0716b500-dd0b-5425-b9d0-d88bc9c09e86","stable_key":"import-f992b796-377d-53bf-a39b-7e5d41dbe194","title":"Pantothenic acid (vitamin B5): coenzyme A, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"b5a49f5a2373ca6064c9a4e014e052dad2f6f199a82bb09b26b82b831c36110b","revision_id":"78f3e793-f084-5ee2-8703-b661c4b650bf","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}