{"id":"d8afb29a-52e6-5e58-a595-1d72bee0da44","stable_key":"research:zdhhc16-palmitoylates-zdhhc6-regulatory-sites","predicate":"palmitoylates","statement":"ZDHHC16 mediates regulatory ZDHHC6 palmitoylation at C328, C329 and C343.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"literature_reviewed:direct_experimental","direction":"neutral","is_public":true,"mechanism_event_id":"7087b602-7d5d-5720-ac61-be85660bcf1d","mechanism_event_label":"Another enzyme adds regulatory lipid groups to ZDHHC6.","subject":{"id":"dbd117d8-d084-5159-b07e-0d7266f5f066","slug":"zdhhc16","display_name":"ZDHHC16","entity_type_key":"protein"},"object":{"id":"a2fd4b35-5483-5e7c-9604-d792fa67d402","slug":"zdhhc6","display_name":"ZDHHC6","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"7087b602-7d5d-5720-ac61-be85660bcf1d","stable_key":"research:zdhhc16-palmitoylates-zdhhc6-regulatory-sites","event_type":"protein-acylation","label":"Another enzyme adds regulatory lipid groups to ZDHHC6.","description":"ZDHHC16 mediates regulatory ZDHHC6 palmitoylation at C328, C329 and C343.","status":"active","compartment":{"slug":"endoplasmic-reticulum","display_name":"Endoplasmic reticulum"},"participants":[{"entity":{"id":"dbd117d8-d084-5159-b07e-0d7266f5f066","slug":"zdhhc16","display_name":"ZDHHC16","entity_type_key":"protein"},"role":"enzyme","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"a2fd4b35-5483-5e7c-9604-d792fa67d402","slug":"zdhhc6","display_name":"ZDHHC6","entity_type_key":"protein"},"role":"substrate","stoichiometry":null,"state_label":"regulatory cysteines","sequence_order":1,"notes":""},{"entity":{"id":"05e9ea97-39b9-5e0a-8dde-6c337bdd67dd","slug":"regulatory-palmitoylated-zdhhc6","display_name":"Regulatory palmitoylated ZDHHC6","entity_type_key":"protein_state"},"role":"product","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"cell_type","value_text":"HeLa and HAP1","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mutagenesis and enzyme perturbations","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Distinct from catalytic DHHC self-acylation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"human","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"ebd05dcf-7fd7-5428-99cd-b9fcc15badc2","evidence_kind":"curated_literature_summary","locator":"lines 882-892","start_line":882,"end_line":892,"excerpt":"## zdhhc16-palmitoylates-zdhhc6-regulatory-sites\n\nAnother enzyme adds regulatory lipid groups to ZDHHC6.\n\nZDHHC16 mediates regulatory ZDHHC6 palmitoylation at C328, C329 and C343.\n\nOrganism: human\nCell type: HeLa and HAP1\nExperimental model: Mutagenesis and enzyme perturbations\nLimitations: Distinct from catalytic DHHC self-acylation.\nPrimary reference: [Identification and dynamics of the human ZDHHC16-ZDHHC6 palmitoylation cascade](https://elifesciences.org/articles/27826)","model_system":"Mutagenesis and enzyme perturbations","directness":"author_interpretation","verification_status":"secondary_verified","notes":"Curated summary; inspect the linked primary papers for original methods and results.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"4f892f13-06ea-5199-a33c-a703f35c80ae","stable_key":"selenium-research-2026-09-17","title":"Selenium: literature corrections and mechanism additions","document_type":"curated_literature_review","citation_label":"Metabolic Ledger literature curation, 17 September 2026; primary papers linked individually","file_path":"","sha256":"0b818b10c1c7120e5caf7f4d4019d7bd025d745692e424f515d3ef903c9ab7f3","revision_id":"80984e03-5f0f-5877-8094-afef7637444e","review_status":"secondary_verified","notes":"Secondary curated summaries of primary experiments, with explicit models and limitations. Not archived primary full text."}}],"relations":[],"conflicts":[],"corrections":[],"research":{"topic":"palmitoylation-regulation","plain_language":"Another enzyme adds regulatory lipid groups to ZDHHC6.","evidence_scope":"direct_experimental","papers":[{"key":"abrami-2017-zdhhc-cascade","title":"Identification and dynamics of the human ZDHHC16-ZDHHC6 palmitoylation cascade","url":"https://elifesciences.org/articles/27826","doi":"10.7554/eLife.27826","year":2017,"model":"Human HeLa/HAP1 perturbations, cysteine mutants and kinetic modeling","summary":"Separates ZDHHC16-mediated regulatory palmitoylation from APT2-dependent removal and measures consequences for ZDHHC6 activity."}]}}