{"id":"d8727349-a197-55c4-9870-e7f63329e8a8","stable_key":"1310afbd-6010-586e-805d-551d846da421:b6-transport-albumin-binding","predicate":"binds","statement":"Equilibrium dialysis found one higher-affinity human-albumin PLP-binding site (Kd 8.7 micromolar) and additional lower-affinity sites.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"394993b2-e08b-5b93-8df8-d362942f443c","mechanism_event_label":"Albumin binds circulating active B6.","subject":{"id":"d74adc7c-c253-5604-99bf-73d5ed0c0701","slug":"human-serum-albumin","display_name":"Human serum albumin","entity_type_key":"protein"},"object":{"id":"6b656ff5-9532-5da4-8eea-8ca163a48649","slug":"pyridoxal-phosphate","display_name":"PLP","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"394993b2-e08b-5b93-8df8-d362942f443c","stable_key":"1310afbd-6010-586e-805d-551d846da421:b6-transport-albumin-binding-event","event_type":"biochemical_relationship","label":"Albumin binds circulating active B6.","description":"Equilibrium dialysis found one higher-affinity human-albumin PLP-binding site (Kd 8.7 micromolar) and additional lower-affinity sites.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"d74adc7c-c253-5604-99bf-73d5ed0c0701","slug":"human-serum-albumin","display_name":"Human serum albumin","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"6b656ff5-9532-5da4-8eea-8ca163a48649","slug":"pyridoxal-phosphate","display_name":"PLP","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_location","value_text":"Indexed abstract: equilibrium dialysis and competition","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Purified human serum albumin binding assays.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"In-vitro albumin/PLP binding.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Purified-system affinity is not a plasma sufficiency threshold; bovine albumin behaved differently.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin B6 research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-b6","display_name":"Vitamin B6","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Albumin binds circulating active B6.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[fonda1991] The binding of pyridoxal 5'-phosphate to human serum albumin. (1991). https://pubmed.ncbi.nlm.nih.gov/1898027/ DOI: 10.1016/0003-9861(91)90167-h","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Purified serum albumin","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"b0c011bd-7d79-547e-98c7-606c268bab03","evidence_kind":"source_excerpt","locator":"Lines 335-346","start_line":335,"end_line":346,"excerpt":"### b6-transport-albumin-binding\nEquilibrium dialysis found one higher-affinity human-albumin PLP-binding site (Kd 8.7 micromolar) and additional lower-affinity sites.\nCondition category: normal\nnutrient_topic: Vitamin B6 research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Albumin binds circulating active B6.\norganism: Homo sapiens\ntissue_or_cell_type: Purified serum albumin\nexperimental_model: Purified human serum albumin binding assays.\nlimitations: Purified-system affinity is not a plasma sufficiency threshold; bovine albumin behaved differently.\nexposure: In-vitro albumin/PLP binding.\nevidence_location: Indexed abstract: equilibrium dialysis and competition\n[fonda1991] The binding of pyridoxal 5'-phosphate to human serum albumin. (1991). https://pubmed.ncbi.nlm.nih.gov/1898027/ DOI: 10.1016/0003-9861(91)90167-h","model_system":"Purified human serum albumin binding assays.","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [fonda1991] The binding of pyridoxal 5'-phosphate to human serum albumin. (1991). https://pubmed.ncbi.nlm.nih.gov/1898027/ DOI: 10.1016/0003-9861(91)90167-h","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"251773bb-16f5-5903-b135-db4a61d9dec4","stable_key":"import-1310afbd-6010-586e-805d-551d846da421","title":"Vitamin B6: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. 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