{"id":"d85925a5-2aa0-5d79-aa4c-1261eced71d9","stable_key":"434e1e27-eb90-583a-956b-62472726ffba:trpv6-direct-calcium-permeation","predicate":"transports","statement":"Rat CaT1, now termed TRPV6, mediates electrogenic calcium uptake when expressed in Xenopus oocytes.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"0f7a10d5-15ec-5b0f-8049-5c53645c72db","mechanism_event_label":"TRPV6 permits calcium entry across a membrane.","subject":{"id":"2f5ca870-de94-5d5e-a9f6-d8da99e26360","slug":"trpv6","display_name":"TRPV6","entity_type_key":"protein"},"object":{"id":"e359bc15-e675-5d83-b0fe-1d70814e130b","slug":"calcium-ion","display_name":"Calcium ion","entity_type_key":"ion"},"evidence_count":1,"mechanism_event":{"id":"0f7a10d5-15ec-5b0f-8049-5c53645c72db","stable_key":"434e1e27-eb90-583a-956b-62472726ffba:trpv6-direct-calcium-permeation-event","event_type":"biochemical_relationship","label":"TRPV6 permits calcium entry across a membrane.","description":"Rat CaT1, now termed TRPV6, mediates electrogenic calcium uptake when expressed in Xenopus oocytes.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"2f5ca870-de94-5d5e-a9f6-d8da99e26360","slug":"trpv6","display_name":"TRPV6","entity_type_key":"protein"},"role":"channel","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"e359bc15-e675-5d83-b0fe-1d70814e130b","slug":"calcium-ion","display_name":"Calcium ion","entity_type_key":"ion"},"role":"transported_ion","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"experimental_model","value_text":"Rat duodenal CaT1/TRPV6 expression in Xenopus oocytes; uptake and electrophysiology","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Heterologous uptake is not proof that every intestinal calcium ion uses this channel.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Calcium research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"calcium","display_name":"Calcium","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Rattus norvegicus channel in Xenopus laevis","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"TRPV6 permits calcium entry across a membrane.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[peng1999] Molecular cloning and characterization of a channel-like transporter mediating intestinal calcium absorption (1999). https://pubmed.ncbi.nlm.nih.gov/10428857/ DOI: 10.1074/jbc.274.32.22739","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Plasma membrane; duodenal channel origin","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"transport_effect","value_text":"raises","comparator":null,"unit":null,"notes":"Mediates electrogenic calcium uptake when expressed.","entity":null},{"dimension":"transport_or_reaction_direction","value_text":"inward","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"transport_pool","value_text":"cytosolic calcium","comparator":null,"unit":null,"notes":"Mediates electrogenic calcium uptake when expressed.","entity":null}],"evidence":[{"id":"9b4cb952-cc46-5456-9de8-d3efb654f825","evidence_kind":"source_excerpt","locator":"Lines 387-397","start_line":387,"end_line":397,"excerpt":"### trpv6-direct-calcium-permeation\nRat CaT1, now termed TRPV6, mediates electrogenic calcium uptake when expressed in Xenopus oocytes.\nCondition category: normal\nnutrient_topic: Calcium research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: TRPV6 permits calcium entry across a membrane.\norganism: Rattus norvegicus channel in Xenopus laevis\ntissue_or_cell_type: Plasma membrane; duodenal channel origin\nexperimental_model: Rat duodenal CaT1/TRPV6 expression in Xenopus oocytes; uptake and electrophysiology\nlimitations: Heterologous uptake is not proof that every intestinal calcium ion uses this channel.\ntransport_or_reaction_direction: inward\n[peng1999] Molecular cloning and characterization of a channel-like transporter mediating intestinal calcium absorption (1999). https://pubmed.ncbi.nlm.nih.gov/10428857/ DOI: 10.1074/jbc.274.32.22739","model_system":"Rat duodenal CaT1/TRPV6 expression in Xenopus oocytes; uptake and electrophysiology","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [peng1999] Molecular cloning and characterization of a channel-like transporter mediating intestinal calcium absorption (1999). https://pubmed.ncbi.nlm.nih.gov/10428857/ DOI: 10.1074/jbc.274.32.22739","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"3c083fa0-59c6-50e9-af42-678d0e3c1006","stable_key":"import-434e1e27-eb90-583a-956b-62472726ffba","title":"Calcium: mechanism-first literature curation (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"965c934d6b147f2b62f8d45ce6d7a87681a600bfe5c46a585b4704889afd3fc1","revision_id":"543ec48b-4f70-5197-9d76-f5a3c443664c","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}