{"id":"d822f878-5345-54f8-86f5-a9e736c35292","stable_key":"b428117d-3a8c-5e74-8711-168153e19545:aspartame-barrier-permeability","predicate":"increases_tested","statement":"Aspartame at 0.1 mM for 24 hours increased Caco-2 monolayer permeability.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"71451c8f-5e04-5130-a43c-c00fad21db5f","mechanism_event_label":"Intact sweetener altered a cultured epithelial barrier.","subject":{"id":"15bd1664-9d4b-514f-ab83-87274a67673f","slug":"aspartame","display_name":"Aspartame","entity_type_key":"small_molecule"},"object":{"id":"8f5d6b57-a2d6-51e9-aa10-0479162ac340","slug":"human-caco2-aspartame-permeability","display_name":"Human Caco-2 permeability during aspartame exposure","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"71451c8f-5e04-5130-a43c-c00fad21db5f","stable_key":"b428117d-3a8c-5e74-8711-168153e19545:aspartame-barrier-permeability-event","event_type":"observed_relationship","label":"Intact sweetener altered a cultured epithelial barrier.","description":"Aspartame at 0.1 mM for 24 hours increased Caco-2 monolayer permeability.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"15bd1664-9d4b-514f-ab83-87274a67673f","slug":"aspartame","display_name":"Aspartame","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"8f5d6b57-a2d6-51e9-aa10-0479162ac340","slug":"human-caco2-aspartame-permeability","display_name":"Human Caco-2 permeability during aspartame exposure","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text; methods and results","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human colon-cancer-derived cell line; dextran flux and electrical resistance assays.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Not a living human gut exposure or clinical disease endpoint.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Aspartame collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"aspartame","display_name":"Aspartame","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Intact sweetener altered a cultured epithelial barrier.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Artificial Sweeteners Disrupt Tight Junctions and Barrier Function in the Intestinal Epithelium through Activation of the Sweet Taste Receptor, T1R3. · 2020 · https://pubmed.ncbi.nlm.nih.gov/32580504/ · DOI 10.3390/nu12061862","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"42e7a99d-fddf-5f51-8b84-f2f453b4f531","evidence_kind":"source_excerpt","locator":"Lines 194-200","start_line":194,"end_line":200,"excerpt":"## aspartame-barrier-permeability\nIntact sweetener altered a cultured epithelial barrier.\nAspartame at 0.1 mM for 24 hours increased Caco-2 monolayer permeability.\nModel: Human colon-cancer-derived cell line; dextran flux and electrical resistance assays.\nLimitations: Not a living human gut exposure or clinical disease endpoint.\nEvidence access: Primary full text; methods and results\nArtificial Sweeteners Disrupt Tight Junctions and Barrier Function in the Intestinal Epithelium through Activation of the Sweet Taste Receptor, T1R3. · 2020 · https://pubmed.ncbi.nlm.nih.gov/32580504/ · DOI 10.3390/nu12061862","model_system":"Human colon-cancer-derived cell line; dextran flux and electrical resistance assays.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"2bba7d36-993f-5ba7-acaf-2e4d573527ed","stable_key":"import-b428117d-3a8c-5e74-8711-168153e19545","title":"Aspartame: digestion, taste, metabolite dependencies and experimental signaling (2026-09-20)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"21cfc6808062c6ea1fb5d34128bcd005d31f0f6c0d63484794238db77d32ae97","revision_id":"1255dda8-df99-5d4b-a65b-4144c47d7d9b","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}