{"id":"d81ec0ea-aeef-5ea9-93b8-34cf36b3167f","stable_key":"be889add-cec8-500b-be89-676431432a70:mn-enz-brain-glul-cofactor-trapping","predicate":"predominates_in_trapped_complex","statement":"Cofactor-trapped GLUL from bovine or ovine brain contained 1.5±0.2 Mg per subunit and less than 0.05 Mn per subunit.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"483c4bbe-aae1-53ed-8f4b-617fce5e9f2b","mechanism_event_label":"A cofactor-trapping study recovered mainly magnesium from brain GLUL.","subject":{"id":"bff427ab-35f9-59c2-bb24-fd5953bbaec2","slug":"magnesium-ion","display_name":"Mg2+","entity_type_key":"ion"},"object":{"id":"8f613a27-6378-5cce-98c4-21b4e13c1708","slug":"ovine-glul","display_name":"Ovine glutamine synthetase / GLUL","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"483c4bbe-aae1-53ed-8f4b-617fce5e9f2b","stable_key":"be889add-cec8-500b-be89-676431432a70:mn-enz-brain-glul-cofactor-trapping-event","event_type":"observed_intervention","label":"A cofactor-trapping study recovered mainly magnesium from brain GLUL.","description":"Cofactor-trapped GLUL from bovine or ovine brain contained 1.5±0.2 Mg per subunit and less than 0.05 Mn per subunit.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"bff427ab-35f9-59c2-bb24-fd5953bbaec2","slug":"magnesium-ion","display_name":"Mg2+","entity_type_key":"ion"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"8f613a27-6378-5cce-98c4-21b4e13c1708","slug":"ovine-glul","display_name":"Ovine glutamine synthetase / GLUL","entity_type_key":"protein"},"role":"object","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"a8bd6374-6947-5c96-92b9-1ca0666175d2","slug":"bovine-glul","display_name":"Bovine glutamine synthetase / GLUL","entity_type_key":"protein"},"role":"second species enzyme","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"a8082b11-c484-5792-bb81-48e8e699cbe4","slug":"manganese-ion","display_name":"Mn2+","entity_type_key":"ion"},"role":"minor or undetectable trapped metal","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"Direct native-metal trapping distinguishes Mg occupancy from Mn-supported activity in vitro.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Cofactor trapping and immunoprecipitation of bovine and ovine brain glutamine synthetase","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Methionine sulfoximine phosphate/ADP cofactor trapping","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Extraction with methionine sulfoximine phosphate and ADP; bovine/ovine brain, not direct living human brain measurement.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Manganese research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"manganese","display_name":"Manganese","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Bos taurus; Ovis aries","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"A cofactor-trapping study recovered mainly magnesium from brain GLUL.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[mn-enz-2870682] Mg2+ is bound to glutamine synthetase extracted from bovine or ovine brain in the presence of L-methionine-S-sulfoximine phosphate. (1986). https://pubmed.ncbi.nlm.nih.gov/2870682/ DOI: 10.1016/0003-9861(86)90496-0","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Brain extracts and purified enzyme","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"0bf4a3bf-b6d2-52ab-9649-aac7e52f103f","evidence_kind":"source_excerpt","locator":"Lines 646-657","start_line":646,"end_line":657,"excerpt":"### mn-enz-brain-glul-cofactor-trapping\nCofactor-trapped GLUL from bovine or ovine brain contained 1.5±0.2 Mg per subunit and less than 0.05 Mn per subunit.\nCondition category: normal\nnutrient_topic: Manganese research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: A cofactor-trapping study recovered mainly magnesium from brain GLUL.\norganism: Bos taurus; Ovis aries\ntissue_or_cell_type: Brain extracts and purified enzyme\nexperimental_model: Cofactor trapping and immunoprecipitation of bovine and ovine brain glutamine synthetase\nlimitations: Extraction with methionine sulfoximine phosphate and ADP; bovine/ovine brain, not direct living human brain measurement.\nexposure: Methionine sulfoximine phosphate/ADP cofactor trapping\ncross_nutrient: Direct native-metal trapping distinguishes Mg occupancy from Mn-supported activity in vitro.\n[mn-enz-2870682] Mg2+ is bound to glutamine synthetase extracted from bovine or ovine brain in the presence of L-methionine-S-sulfoximine phosphate. (1986). https://pubmed.ncbi.nlm.nih.gov/2870682/ DOI: 10.1016/0003-9861(86)90496-0","model_system":"Cofactor trapping and immunoprecipitation of bovine and ovine brain glutamine synthetase","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [mn-enz-2870682] Mg2+ is bound to glutamine synthetase extracted from bovine or ovine brain in the presence of L-methionine-S-sulfoximine phosphate. 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