{"id":"d7c685e6-48b0-5b0a-ab7e-6235c0dd6ad8","stable_key":"74ef5bba-ee14-531c-b1bf-d1f297c56bb6:fisetin-imr90-etoposide","predicate":"reduces","statement":"Fisetin at 1-15 micromolar reduced the senescence-marker-positive population in etoposide-treated human IMR90 cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"9c079f25-4303-545f-89ab-5f54eab2f4ee","mechanism_event_label":"A different induction protocol produced a different response.","subject":{"id":"dee05061-c3f3-5226-8054-dd58b76a585d","slug":"fisetin","display_name":"Fisetin","entity_type_key":"small_molecule"},"object":{"id":"9e5bfd29-f721-582e-b576-43f4de35f877","slug":"human-imr90-etoposide-senescence-markers","display_name":"Senescence-marker-positive human IMR90 cells after etoposide","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"9c079f25-4303-545f-89ab-5f54eab2f4ee","stable_key":"74ef5bba-ee14-531c-b1bf-d1f297c56bb6:fisetin-imr90-etoposide-event","event_type":"observed_relationship","label":"A different induction protocol produced a different response.","description":"Fisetin at 1-15 micromolar reduced the senescence-marker-positive population in etoposide-treated human IMR90 cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"dee05061-c3f3-5226-8054-dd58b76a585d","slug":"fisetin","display_name":"Fisetin","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"9e5bfd29-f721-582e-b576-43f4de35f877","slug":"human-imr90-etoposide-senescence-markers","display_name":"Senescence-marker-positive human IMR90 cells after etoposide","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"20 micromolar etoposide for 24 hours; fisetin assessed later over 48 hours.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Marker-positive cell counts do not alone identify killing versus altered marker expression.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Fisetin collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"fisetin","display_name":"Fisetin","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A different induction protocol produced a different response.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Fisetin is a senotherapeutic that extends health and lifespan. · 2018 · https://pubmed.ncbi.nlm.nih.gov/30279143/ · DOI 10.1016/j.ebiom.2018.09.015","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"a874b12c-031e-5109-8fe4-0728a92974bc","evidence_kind":"source_excerpt","locator":"Lines 200-206","start_line":200,"end_line":206,"excerpt":"## fisetin-imr90-etoposide\nA different induction protocol produced a different response.\nFisetin at 1-15 micromolar reduced the senescence-marker-positive population in etoposide-treated human IMR90 cells.\nModel: 20 micromolar etoposide for 24 hours; fisetin assessed later over 48 hours.\nLimitations: Marker-positive cell counts do not alone identify killing versus altered marker expression.\nEvidence access: Primary full text\nFisetin is a senotherapeutic that extends health and lifespan. · 2018 · https://pubmed.ncbi.nlm.nih.gov/30279143/ · DOI 10.1016/j.ebiom.2018.09.015","model_system":"20 micromolar etoposide for 24 hours; fisetin assessed later over 48 hours.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"1108bfff-6504-5152-a51f-ff3d7c27c282","stable_key":"import-74ef5bba-ee14-531c-b1bf-d1f297c56bb6","title":"Fisetin: metabolism, cell-state responses and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"c30e0764cd34bd062cfcbfaf32357ef29dfda30e7d4153c545178140f5054e09","revision_id":"40ff52c0-f4b9-5820-8abb-ff002c6364a4","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}