{"id":"d7bfcb01-e54a-5f6e-b4ca-2f51a97ce0a4","stable_key":"c156003d-8cdf-5259-8561-9803795ecc82:pdk4-pdh-control","predicate":"increases","statement":"PDK4 overexpression increased PDH phosphorylation in neonatal rat cardiomyocytes.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"b67b1bea-1310-55f3-a876-1401012302f9","mechanism_event_label":"pdk4 pdh control","subject":{"id":"e1f96aa2-37f7-5f22-a7bc-84928da5a92d","slug":"rat-pdk4-overexpression","display_name":"PDK4 overexpression in neonatal rat ventricular cardiomyocytes","entity_type_key":"protein_state"},"object":{"id":"7ed839e6-2464-53b6-ad14-e0da8e58fb43","slug":"rat-pdh-phosphorylation","display_name":"Rat cardiomyocyte PDH phosphorylation","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"b67b1bea-1310-55f3-a876-1401012302f9","stable_key":"c156003d-8cdf-5259-8561-9803795ecc82:pdk4-pdh-control","event_type":"increases","label":"pdk4 pdh control","description":"Model-specific finding; read linked claim context and source-access tier.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"e1f96aa2-37f7-5f22-a7bc-84928da5a92d","slug":"rat-pdk4-overexpression","display_name":"PDK4 overexpression in neonatal rat ventricular cardiomyocytes","entity_type_key":"protein_state"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"7ed839e6-2464-53b6-ad14-e0da8e58fb43","slug":"rat-pdh-phosphorylation","display_name":"Rat cardiomyocyte PDH phosphorylation","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"access_level","value_text":"full_text_and_supplement_review","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"compartment","value_text":"Mitochondria or cell lysate, depending on assay","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"dose","value_text":"Adenovirus MOI 50:1 where applicable; compound concentrations not specified in reviewed methods","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"Adenovirus incubation 6–8 h; subsequent endpoint timing not consistently specified","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"endpoint","value_text":"rat-pdh-phosphorylation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_location","value_text":"Supplementary Figure S8C/D","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Adenoviral/cell perturbation study","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"rat-pdk4-overexpression","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Single primary study; not independently replicated here. Cardiac and recombinant findings do not establish pulmonary endothelial, viral-sepsis or ARDS effects. Phosphorylation is not a direct PDH flux measurement. Peer-review response wording and panel labels are inconsistent; retain the main-text assignment with this qualification.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"Rattus norvegicus host cells; construct species may be unspecified","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"PDK4 overexpression increased PDH phosphorylation in neonatal rat cardiomyocytes.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_locator","value_text":"[{\"cache\": \"artifacts/dlat-curation/heart-blocks.json\", \"block_index\": 32, \"text_sha256\": \"ea1ec18a5210aa68822ce1416ae850de5ce871e100938a13a2c1dcc49c444731\", \"xml_element_id\": \"Par14\"}]","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"https://doi.org/10.1038/s41467-026-70703-w","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Neonatal rat ventricular cardiomyocytes (NRVCMs)","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"eb0f980f-76cb-5656-805e-13335950964f","evidence_kind":"source_excerpt","locator":"Lines 284-298","start_line":284,"end_line":298,"excerpt":"PDK4 overexpression increased PDH phosphorylation in neonatal rat cardiomyocytes.\norganism: Rattus norvegicus host cells; construct species may be unspecified\ntissue_or_cell_type: Neonatal rat ventricular cardiomyocytes (NRVCMs)\nexperimental_model: Adenoviral/cell perturbation study\ncompartment: Mitochondria or cell lysate, depending on assay\ndose: Adenovirus MOI 50:1 where applicable; compound concentrations not specified in reviewed methods\nduration: Adenovirus incubation 6–8 h; subsequent endpoint timing not consistently specified\nprimary_references: https://doi.org/10.1038/s41467-026-70703-w\naccess_level: full_text_and_supplement_review\nevidence_location: Supplementary Figure S8C/D\nendpoint: rat-pdh-phosphorylation\nexposure: rat-pdk4-overexpression\nlimitations: Single primary study; not independently replicated here. Cardiac and recombinant findings do not establish pulmonary endothelial, viral-sepsis or ARDS effects. Phosphorylation is not a direct PDH flux measurement. Peer-review response wording and panel labels are inconsistent; retain the main-text assignment with this qualification.\nprimary_locator: [{\"cache\": \"artifacts/dlat-curation/heart-blocks.json\", \"block_index\": 32, \"text_sha256\": \"ea1ec18a5210aa68822ce1416ae850de5ce871e100938a13a2c1dcc49c444731\", \"xml_element_id\": \"Par14\"}]\nplain_language: PDK4 overexpression increased PDH phosphorylation in neonatal rat cardiomyocytes.","model_system":"Adenoviral/cell perturbation study","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Quotation is the authored curation, not original publisher wording. Exact primary locators and access limits retained in contexts.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"ee72956d-4fd4-58d3-9a94-e173bb1e3282","stable_key":"import-c156003d-8cdf-5259-8561-9803795ecc82","title":"DLAT: cardiac fatty-acid oxidation and mitochondrial glutathione evidence","document_type":"imported_text","citation_label":"AI-assisted two-paper curation, 2026-09-20. Cardiac full-text/supplement review; CRC abstract only. No pulmonary endothelial validation.","file_path":"","sha256":"159e5472c8b1d104625f9c648dc9ee24dcd5fe102109fd960bba50e9ba9de954","revision_id":"5ca6b1f1-fd75-5f8d-ba2b-0215fbf387c7","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}