{"id":"d780c4d7-f49a-57c9-a347-b17e5e8a387a","stable_key":"3279ff78-2460-5064-bfc0-3f2987af85a0:melatonin-asmt-isoform-5mtp","predicate":"supports_production_of","statement":"Restoring HIOMT298 in A549 cells restored 5-methoxytryptophan production.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"7dd945b0-2c15-5850-afd0-081965510df1","mechanism_event_label":"An alternatively spliced form connects to a different tryptophan product.","subject":{"id":"d0b73e5d-744e-5d45-9983-6918968436a7","slug":"asmt-hiomt298","display_name":"Human HIOMT298 / ASMT 298-amino-acid splice isoform","entity_type_key":"protein_state"},"object":{"id":"4f23b054-ec02-57fc-89bd-55d851bec8d1","slug":"5-methoxytryptophan","display_name":"5-Methoxytryptophan / 5-MTP","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"7dd945b0-2c15-5850-afd0-081965510df1","stable_key":"3279ff78-2460-5064-bfc0-3f2987af85a0:melatonin-asmt-isoform-5mtp-event","event_type":"biochemical_relationship","label":"An alternatively spliced form connects to a different tryptophan product.","description":"Restoring HIOMT298 in A549 cells restored 5-methoxytryptophan production.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"d0b73e5d-744e-5d45-9983-6918968436a7","slug":"asmt-hiomt298","display_name":"Human HIOMT298 / ASMT 298-amino-acid splice isoform","entity_type_key":"protein_state"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"4f23b054-ec02-57fc-89bd-55d851bec8d1","slug":"5-methoxytryptophan","display_name":"5-Methoxytryptophan / 5-MTP","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/melatonin-research/29794137.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"051e28814382bea96e610c4cc406e7bc1d4d705d245b43dea0bba61050ae341e\", \"start_char\": 0, \"end_char\": 1575, \"text_sha256\": \"051e28814382bea96e610c4cc406e7bc1d4d705d245b43dea0bba61050ae341e\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Isoform expression, transfection and metabolic measurements","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"HIOMT298 expression versus pineal HIOMT345","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The 298-aa isoform was studied for 5-MTP production. Do not assign its anti-tumor findings to melatonin or assume all ASMT isoforms have identical products.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Melatonin research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"melatonin","display_name":"Melatonin","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human fibroblasts, A549 cancer cells and mouse xenografts","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"An alternatively spliced form connects to a different tryptophan product.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[melatonin-p29794137] Restoration of hydroxyindole O-methyltransferase levels in human cancer cells induces a tryptophan-metabolic switch and attenuates cancer progression. 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Do not assign its anti-tumor findings to melatonin or assume all ASMT isoforms have identical products.\nexposure: HIOMT298 expression versus pineal HIOMT345\nevidence_span: {\"source_cache\": \"artifacts/melatonin-research/29794137.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"051e28814382bea96e610c4cc406e7bc1d4d705d245b43dea0bba61050ae341e\", \"start_char\": 0, \"end_char\": 1575, \"text_sha256\": \"051e28814382bea96e610c4cc406e7bc1d4d705d245b43dea0bba61050ae341e\"}\n[melatonin-p29794137] Restoration of hydroxyindole O-methyltransferase levels in human cancer cells induces a tryptophan-metabolic switch and attenuates cancer progression. 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