{"id":"d769586d-78b8-533e-ba86-e1b3f815010c","stable_key":"6d38d43e-01e4-5641-93be-65654271e242:zinc-trans-zip4-mrna-stability","predicate":"deficiency_stabilizes","statement":"Dietary zinc deficiency increased mouse Zip4 mRNA by greater transcript stability rather than an increased relative transcription rate.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"6b738465-14e5-51ef-a2da-d8a33fe0150d","mechanism_event_label":"With little dietary zinc, mice preserve the message used to make ZIP4.","subject":{"id":"0429f0ff-c033-514b-9a4f-6f0f83268095","slug":"zinc","display_name":"Zinc","entity_type_key":"nutrient_element"},"object":{"id":"62af30e7-eb6e-5839-b905-cdefb555295a","slug":"mouse-slc39a4-mrna","display_name":"Mouse Slc39a4 messenger RNA","entity_type_key":"rna"},"evidence_count":1,"mechanism_event":{"id":"6b738465-14e5-51ef-a2da-d8a33fe0150d","stable_key":"6d38d43e-01e4-5641-93be-65654271e242:zinc-trans-zip4-mrna-stability-event","event_type":"biochemical_relationship","label":"With little dietary zinc, mice preserve the message used to make ZIP4.","description":"Dietary zinc deficiency increased mouse Zip4 mRNA by greater transcript stability rather than an increased relative transcription rate.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"b2399154-5b80-5772-bf7c-d9d80aefb691","slug":"mouse-slc39a4","display_name":"Mouse ZIP4 (Slc39a4)","entity_type_key":"protein"},"role":"encoded_transporter","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"0429f0ff-c033-514b-9a4f-6f0f83268095","slug":"zinc","display_name":"Zinc","entity_type_key":"nutrient_element"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"62af30e7-eb6e-5839-b905-cdefb555295a","slug":"mouse-slc39a4-mrna","display_name":"Mouse Slc39a4 messenger RNA","entity_type_key":"rna"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"cross_nutrient","value_text":"false","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mouse intestine with nuclear run-on assays; supporting Hepa-cell analyses","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Defined zinc-deficient versus adequate diets; transcription assessed after 24 hours from gestational day 8.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Transcript stabilization in mice is not a serum-zinc threshold or proof that all zinc deficiency responses are post-transcriptional.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Zinc research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"zinc","display_name":"Zinc","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Mus musculus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"With little dietary zinc, mice preserve the message used to make ZIP4.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[zinc-trans-18020946] Novel zinc-responsive post-transcriptional mechanisms reciprocally regulate expression of the mouse Slc39a4 and Slc39a5 zinc transporters (Zip4 and Zip5). (2007). https://pubmed.ncbi.nlm.nih.gov/18020946/ DOI: 10.1515/bc.2007.149","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Small intestine; supporting cultured hepatic cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"7ca1bde0-b473-5c46-8c86-3975a02ddadf","evidence_kind":"source_excerpt","locator":"Lines 232-243","start_line":232,"end_line":243,"excerpt":"### zinc-trans-zip4-mrna-stability\nDietary zinc deficiency increased mouse Zip4 mRNA by greater transcript stability rather than an increased relative transcription rate.\nCondition category: nutrient_deficiency\nnutrient_topic: Zinc research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: With little dietary zinc, mice preserve the message used to make ZIP4.\norganism: Mus musculus\ntissue_or_cell_type: Small intestine; supporting cultured hepatic cells\nexperimental_model: Mouse intestine with nuclear run-on assays; supporting Hepa-cell analyses\nlimitations: Transcript stabilization in mice is not a serum-zinc threshold or proof that all zinc deficiency responses are post-transcriptional.\nexposure: Defined zinc-deficient versus adequate diets; transcription assessed after 24 hours from gestational day 8.\ncross_nutrient: false\n[zinc-trans-18020946] Novel zinc-responsive post-transcriptional mechanisms reciprocally regulate expression of the mouse Slc39a4 and Slc39a5 zinc transporters (Zip4 and Zip5). (2007). https://pubmed.ncbi.nlm.nih.gov/18020946/ DOI: 10.1515/bc.2007.149","model_system":"Mouse intestine with nuclear run-on assays; supporting Hepa-cell analyses","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [zinc-trans-18020946] Novel zinc-responsive post-transcriptional mechanisms reciprocally regulate expression of the mouse Slc39a4 and Slc39a5 zinc transporters (Zip4 and Zip5). (2007). https://pubmed.ncbi.nlm.nih.gov/18020946/ DOI: 10.1515/bc.2007.149","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"c5ee0fee-ce5c-58de-905a-10fb0ea0723c","stable_key":"import-6d38d43e-01e4-5641-93be-65654271e242","title":"Zinc: transport, enzyme loading, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"2e731dd54477ec1254e97df3323e1208d38f1375effed19252e71ab4f600d13a","revision_id":"c72258b9-ac09-5408-9d44-a921ad1f96a3","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}