{"id":"d75db905-5fa5-5c06-a292-1570a6333919","stable_key":"2921131d-0ba0-51d3-8f59-8c4be3b9ac08:cucurbitacin-b-cys-retained-activity","predicate":"reduces_tested","statement":"The cucurbitacin B cysteine conjugate retained antiproliferative activity in tested HepG2, MCF7 and PANC1 cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"96f56ae7-e6d9-5029-b31f-f3fd5fd1b632","mechanism_event_label":"Conjugation did not automatically erase every biological effect.","subject":{"id":"5c1c8ce6-cd86-5217-bb96-bc4665fc1730","slug":"cucurbitacin-b-cysteine-conjugate","display_name":"Cucurbitacin B cysteine conjugate","entity_type_key":"small_molecule"},"object":{"id":"e6582307-5698-544b-a89d-ccc7f97f325c","slug":"human-cub-cys-cell-proliferation","display_name":"Human cancer-cell proliferation during CuB-cysteine conjugate exposure","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"96f56ae7-e6d9-5029-b31f-f3fd5fd1b632","stable_key":"2921131d-0ba0-51d3-8f59-8c4be3b9ac08:cucurbitacin-b-cys-retained-activity-event","event_type":"observed_relationship","label":"Conjugation did not automatically erase every biological effect.","description":"The cucurbitacin B cysteine conjugate retained antiproliferative activity in tested HepG2, MCF7 and PANC1 cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"5c1c8ce6-cd86-5217-bb96-bc4665fc1730","slug":"cucurbitacin-b-cysteine-conjugate","display_name":"Cucurbitacin B cysteine conjugate","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"e6582307-5698-544b-a89d-ccc7f97f325c","slug":"human-cub-cys-cell-proliferation","display_name":"Human cancer-cell proliferation during CuB-cysteine conjugate exposure","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human cancer-cell models of an identified metabolite.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Not evidence of clinical efficacy or selectivity for cancer over authenticated normal cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Cucurbitacins collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"cucurbitacin","display_name":"Cucurbitacins","entity_type_key":"chemical_species"}},{"dimension":"plain_language","value_text":"Conjugation did not automatically erase every biological effect.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Metabolic fate of the natural anticancer agent cucurbitacin B: an LC-MS/MS-enabled profiling of its major phase I and II conjugates in vivo. · 2024 · https://pubmed.ncbi.nlm.nih.gov/39441433/ · DOI 10.1007/s00216-024-05608-y","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"96b18747-d487-59a0-987b-d0bf320cc2cb","evidence_kind":"source_excerpt","locator":"Lines 380-386","start_line":380,"end_line":386,"excerpt":"## cucurbitacin-b-cys-retained-activity\nConjugation did not automatically erase every biological effect.\nThe cucurbitacin B cysteine conjugate retained antiproliferative activity in tested HepG2, MCF7 and PANC1 cells.\nModel: Human cancer-cell models of an identified metabolite.\nLimitations: Not evidence of clinical efficacy or selectivity for cancer over authenticated normal cells.\nEvidence access: Primary abstract\nMetabolic fate of the natural anticancer agent cucurbitacin B: an LC-MS/MS-enabled profiling of its major phase I and II conjugates in vivo. · 2024 · https://pubmed.ncbi.nlm.nih.gov/39441433/ · DOI 10.1007/s00216-024-05608-y","model_system":"Human cancer-cell models of an identified metabolite.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"77324310-00ce-5d28-9fc7-595fddae86b9","stable_key":"import-2921131d-0ba0-51d3-8f59-8c4be3b9ac08","title":"Cucurbitacins: thiol chemistry, cytoskeleton, metabolic dependencies and signaling (2026-09-20)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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