{"id":"d705c998-7c6c-5e53-9d39-8b3ab2341295","stable_key":"46d15d9e-d3b5-544d-ba01-b785aa3e4f42:thiamine-def-brain-ros-increase","predicate":"antagonist-assisted-depletion-increases","statement":"Reactive oxygen species assay signals were higher in thalamus and cortex on day 14 of pyrithiamine-assisted deficiency.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"01526de2-cfe5-579e-9a2f-ff1b9407fd5a","mechanism_event_label":"Late symptomatic depletion increased a marker of oxidant production in both sampled regions.","subject":{"id":"36b7c3eb-068b-500f-af1c-078829df1ecc","slug":"thiamine","display_name":"Thiamine (vitamin B1)","entity_type_key":"small_molecule"},"object":{"id":"075632fc-ab3c-51b6-b430-81a48cab61d6","slug":"brain-reactive-oxygen-species-signal","display_name":"Brain reactive oxygen species assay signal","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"01526de2-cfe5-579e-9a2f-ff1b9407fd5a","stable_key":"46d15d9e-d3b5-544d-ba01-b785aa3e4f42:thiamine-def-brain-ros-increase-event","event_type":"biochemical_relationship","label":"Late symptomatic depletion increased a marker of oxidant production in both sampled regions.","description":"Reactive oxygen species assay signals were higher in thalamus and cortex on day 14 of pyrithiamine-assisted deficiency.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"75a7f374-5ae9-5565-9f52-388e627bb492","slug":"pyrithiamine","display_name":"Pyrithiamine","entity_type_key":"small_molecule"},"role":"experimental antagonist","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"36b7c3eb-068b-500f-af1c-078829df1ecc","slug":"thiamine","display_name":"Thiamine (vitamin B1)","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"075632fc-ab3c-51b6-b430-81a48cab61d6","slug":"brain-reactive-oxygen-species-signal","display_name":"Brain reactive oxygen species assay signal","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_location","value_text":"Abstract; Methods; Figure 1A","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_span","value_text":"significantly elevated in thalamus and cortex on day 14","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Male Sprague-Dawley rats, eight weeks old; thiamine-deficient chow plus daily pyrithiamine 0.25 mg/kg intraperitoneally; pair-fed thiamine-injected controls; days 13 and 14 brain assays.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Thiamine-deficient chow plus pyrithiamine, day 14 with seizures and absent righting reflex","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The assay does not identify one oxidant species or its initiating enzyme; seizures can also influence the endpoint.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Thiamine research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"thiamine","display_name":"Thiamine (vitamin B1)","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Rattus norvegicus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Late symptomatic depletion increased a marker of oxidant production in both sampled regions.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[langlais-1997-brain-oxidants] Increased cerebral free radical production during thiamine deficiency (1997). https://pubmed.ncbi.nlm.nih.gov/9203158/ DOI: 10.1007/BF02674735","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Thalamus and cortex","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"1d18db07-847d-5cfb-93b3-c703ec8666fb","evidence_kind":"source_excerpt","locator":"Lines 1155-1167","start_line":1155,"end_line":1167,"excerpt":"### thiamine-def-brain-ros-increase\nReactive oxygen species assay signals were higher in thalamus and cortex on day 14 of pyrithiamine-assisted deficiency.\nCondition category: nutrient_deficiency\nnutrient_topic: Thiamine research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Late symptomatic depletion increased a marker of oxidant production in both sampled regions.\norganism: Rattus norvegicus\ntissue_or_cell_type: Thalamus and cortex\nexperimental_model: Male Sprague-Dawley rats, eight weeks old; thiamine-deficient chow plus daily pyrithiamine 0.25 mg/kg intraperitoneally; pair-fed thiamine-injected controls; days 13 and 14 brain assays.\nlimitations: The assay does not identify one oxidant species or its initiating enzyme; seizures can also influence the endpoint.\nevidence_location: Abstract; Methods; Figure 1A\nevidence_span: significantly elevated in thalamus and cortex on day 14\nexposure: Thiamine-deficient chow plus pyrithiamine, day 14 with seizures and absent righting reflex\n[langlais-1997-brain-oxidants] Increased cerebral free radical production during thiamine deficiency (1997). https://pubmed.ncbi.nlm.nih.gov/9203158/ DOI: 10.1007/BF02674735","model_system":"Male Sprague-Dawley rats, eight weeks old; thiamine-deficient chow plus daily pyrithiamine 0.25 mg/kg intraperitoneally; pair-fed thiamine-injected controls; days 13 and 14 brain assays.","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [langlais-1997-brain-oxidants] Increased cerebral free radical production during thiamine deficiency (1997). https://pubmed.ncbi.nlm.nih.gov/9203158/ DOI: 10.1007/BF02674735","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"158d2c03-ac8c-589f-8270-c468165ae346","stable_key":"import-46d15d9e-d3b5-544d-ba01-b785aa3e4f42","title":"Thiamine: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"f376512fb3310141315548015b387833e3af45146e73fb73cd02cee20e4ddb9c","revision_id":"53bc5eda-dec8-58cc-a56f-a9eb4ab036ef","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}