{"id":"d6cd4f54-01ff-5467-b6af-2f450322b748","stable_key":"548ab9d6-3a9b-5bed-879c-17d03813b636:b2-rat-iron-partition-preserved","predicate":"does-not-alter-tested","statement":"Thirty minutes after iron dosing, riboflavin-deficient rats had less mucosal 59Fe but unchanged relative partition between ferritin and transferrin.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"f95f16ab-5220-5e62-a6fc-e382e8c31050","mechanism_event_label":"The uptake decrease was not explained by the tested redistribution hypothesis.","subject":{"id":"86eb1eee-a8d1-539c-8c17-0911f69b6f1b","slug":"riboflavin","display_name":"Riboflavin (vitamin B2)","entity_type_key":"small_molecule"},"object":{"id":"3828dd4b-270a-5427-ae74-5c653d58c402","slug":"intestinal-mucosal-iron-partition","display_name":"Intestinal mucosal iron partition","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"f95f16ab-5220-5e62-a6fc-e382e8c31050","stable_key":"548ab9d6-3a9b-5bed-879c-17d03813b636:b2-rat-iron-partition-preserved-event","event_type":"biochemical_relationship","label":"The uptake decrease was not explained by the tested redistribution hypothesis.","description":"Thirty minutes after iron dosing, riboflavin-deficient rats had less mucosal 59Fe but unchanged relative partition between ferritin and transferrin.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"89bcaf42-b4ab-5760-8c2e-44eace10cee0","slug":"iron","display_name":"Iron","entity_type_key":"nutrient_element"},"role":"tracer","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"5ee66abb-7d76-5b09-bd06-77d39d0e3bcd","slug":"mammalian-ferritin","display_name":"Mammalian ferritin protein complexes","entity_type_key":"protein_family"},"role":"one measured iron-binding pool","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"86eb1eee-a8d1-539c-8c17-0911f69b6f1b","slug":"riboflavin","display_name":"Riboflavin (vitamin B2)","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"3828dd4b-270a-5427-ae74-5c653d58c402","slug":"intestinal-mucosal-iron-partition","display_name":"Intestinal mucosal iron partition","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"cross_nutrient","value_text":"Constrains the proposed B2-iron mechanism.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_location","value_text":"Abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Dietary riboflavin-deficient male rats versus age-/weight-matched controls; intragastric 59Fe and mucosal brush-border vesicles.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Dietary riboflavin deficiency; 30-minute tracer endpoint","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Only the reported pools and time point were compared.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Riboflavin research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"riboflavin","display_name":"Riboflavin (vitamin B2)","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Rattus norvegicus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The uptake decrease was not explained by the tested redistribution hypothesis.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[butler1993] Comparison of changes in the uptake and mucosal processing of iron in riboflavin-deficient rats. (1993). https://pubmed.ncbi.nlm.nih.gov/8358336/","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Intestinal mucosa","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"9d90db0c-cf7f-5e7b-ae6e-e64e48ee0c9f","evidence_kind":"source_excerpt","locator":"Lines 1430-1442","start_line":1430,"end_line":1442,"excerpt":"### b2-rat-iron-partition-preserved\nThirty minutes after iron dosing, riboflavin-deficient rats had less mucosal 59Fe but unchanged relative partition between ferritin and transferrin.\nCondition category: nutrient_deficiency\nnutrient_topic: Riboflavin research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The uptake decrease was not explained by the tested redistribution hypothesis.\norganism: Rattus norvegicus\ntissue_or_cell_type: Intestinal mucosa\nexperimental_model: Dietary riboflavin-deficient male rats versus age-/weight-matched controls; intragastric 59Fe and mucosal brush-border vesicles.\nlimitations: Only the reported pools and time point were compared.\nexposure: Dietary riboflavin deficiency; 30-minute tracer endpoint\ncross_nutrient: Constrains the proposed B2-iron mechanism.\nevidence_location: Abstract\n[butler1993] Comparison of changes in the uptake and mucosal processing of iron in riboflavin-deficient rats. (1993). https://pubmed.ncbi.nlm.nih.gov/8358336/","model_system":"Dietary riboflavin-deficient male rats versus age-/weight-matched controls; intragastric 59Fe and mucosal brush-border vesicles.","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [butler1993] Comparison of changes in the uptake and mucosal processing of iron in riboflavin-deficient rats. (1993). https://pubmed.ncbi.nlm.nih.gov/8358336/","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"4f7c9578-82bf-5e2d-b5c4-72a79fb4f6af","stable_key":"import-548ab9d6-3a9b-5bed-879c-17d03813b636","title":"Riboflavin: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. 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