{"id":"d69164bf-fcdc-5615-8d70-8fb5d640a4d7","stable_key":"220adaab-10d8-5c4e-a3f5-a48700ea794d:agmatine-sulfate-nos3-inhibition","predicate":"competitively_inhibits","statement":"Agmatine competitively inhibited endothelial-derived NOS III only at a much higher approximate Ki of 7.5 mM in the isoform comparison.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"c2106282-2a68-5c0a-989e-c3fa8415fcc7","mechanism_event_label":"Direct inhibition of one isoform can require a very different concentration.","subject":{"id":"e2f83eb4-99ed-5050-87d3-3e4701d05c1a","slug":"agmatine","display_name":"Agmatine","entity_type_key":"small_molecule"},"object":{"id":"1b08d15c-0b9c-5f77-b2e0-085a848439a1","slug":"nos3-1996-agmatine-assay","display_name":"Endothelial-derived NOS III in the 1996 agmatine assay, species unverified","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"c2106282-2a68-5c0a-989e-c3fa8415fcc7","stable_key":"220adaab-10d8-5c4e-a3f5-a48700ea794d:agmatine-sulfate-nos3-inhibition-event","event_type":"observed_relationship","label":"Direct inhibition of one isoform can require a very different concentration.","description":"Agmatine competitively inhibited endothelial-derived NOS III only at a much higher approximate Ki of 7.5 mM in the isoform comparison.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"e2f83eb4-99ed-5050-87d3-3e4701d05c1a","slug":"agmatine","display_name":"Agmatine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"1b08d15c-0b9c-5f77-b2e0-085a848439a1","slug":"nos3-1996-agmatine-assay","display_name":"Endothelial-derived NOS III in the 1996 agmatine assay, species unverified","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"ae2dddf4-ce04-57e3-8a06-7ed9fdc24554","slug":"agmatine-sulfate","display_name":"Agmatine Sulfate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"eeca3c9e-7749-5677-b7bd-37fe8f7c228d","slug":"arginine","display_name":"L-Arginine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Endothelial NOS enzyme preparation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"This enzyme result is distinct from upstream signaling in intact endothelial cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Agmatine Sulfate collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"agmatine-sulfate","display_name":"Agmatine Sulfate","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Direct inhibition of one isoform can require a very different concentration.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Inhibition of mammalian nitric oxide synthases by agmatine, an endogenous polyamine formed by decarboxylation of arginine. · 1996 · https://pubmed.ncbi.nlm.nih.gov/8645212/ · DOI 10.1042/bj3160247","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"cecae5a3-abad-5a74-ae35-d1d87995ebb6","evidence_kind":"source_excerpt","locator":"Lines 236-242","start_line":236,"end_line":242,"excerpt":"## agmatine-sulfate-nos3-inhibition\nDirect inhibition of one isoform can require a very different concentration.\nAgmatine competitively inhibited endothelial-derived NOS III only at a much higher approximate Ki of 7.5 mM in the isoform comparison.\nModel: Endothelial NOS enzyme preparation.\nLimitations: This enzyme result is distinct from upstream signaling in intact endothelial cells.\nEvidence access: Primary abstract\nInhibition of mammalian nitric oxide synthases by agmatine, an endogenous polyamine formed by decarboxylation of arginine. · 1996 · https://pubmed.ncbi.nlm.nih.gov/8645212/ · DOI 10.1042/bj3160247","model_system":"Endothelial NOS enzyme preparation.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"e0426971-7d13-51c9-8354-6547b664f1cf","stable_key":"import-220adaab-10d8-5c4e-a3f5-a48700ea794d","title":"Agmatine Sulfate: transport, guanidino metabolism, ion channels and cross-nutrient mechanisms (2026-09-20)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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