{"id":"d6142962-611b-53b4-a388-4177a4e93652","stable_key":"56f1d914-e7da-595a-af69-c217b2b47407:l-phenylalanine-lacphe-obese-mice","predicate":"reduces","statement":"Injected Lac-Phe at 50 mg/kg reduced food intake in diet-induced obese mice; daily injection for ten days reduced intake, adiposity and weight.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"b6d267d3-119f-538d-8fbc-78d7dd5a8c0c","mechanism_event_label":"The combined metabolite changed feeding in a specific mouse model.","subject":{"id":"2e1e9f48-df5d-5d8a-a1e9-96e90a6eb6d1","slug":"n-lactoyl-phenylalanine","display_name":"N-Lactoyl-phenylalanine / Lac-Phe","entity_type_key":"small_molecule"},"object":{"id":"32a040e8-753e-5ef3-b9b1-2648c304082a","slug":"mouse-lacphe-food-intake","display_name":"Mouse food intake during Lac-Phe administration","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"b6d267d3-119f-538d-8fbc-78d7dd5a8c0c","stable_key":"56f1d914-e7da-595a-af69-c217b2b47407:l-phenylalanine-lacphe-obese-mice-event","event_type":"observed_relationship","label":"The combined metabolite changed feeding in a specific mouse model.","description":"Injected Lac-Phe at 50 mg/kg reduced food intake in diet-induced obese mice; daily injection for ten days reduced intake, adiposity and weight.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"2e1e9f48-df5d-5d8a-a1e9-96e90a6eb6d1","slug":"n-lactoyl-phenylalanine","display_name":"N-Lactoyl-phenylalanine / Lac-Phe","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"32a040e8-753e-5ef3-b9b1-2648c304082a","slug":"mouse-lacphe-food-intake","display_name":"Mouse food intake during Lac-Phe administration","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"190407ad-0219-54b5-b05f-7c75f3895ca6","slug":"l-phenylalanine","display_name":"L-Phenylalanine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"5ebd9a7c-b151-5b70-b70d-7d33067d4ebf","slug":"lactate","display_name":"L-Lactate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Intraperitoneal pharmacological dosing in diet-induced obese mice.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Oral dosing did not reproduce the effect; lean mice did not respond even up to 150 mg/kg. Lactate and phenylalanine separately did not reproduce it.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Phenylalanine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-phenylalanine","display_name":"L-Phenylalanine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The combined metabolite changed feeding in a specific mouse model.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"An exercise-inducible metabolite that suppresses feeding and obesity. · 2022 · https://pubmed.ncbi.nlm.nih.gov/35705806/ · DOI 10.1038/s41586-022-04828-5","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"ab9ee217-7397-51b4-9890-ad0e8409c072","evidence_kind":"source_excerpt","locator":"Lines 406-412","start_line":406,"end_line":412,"excerpt":"## l-phenylalanine-lacphe-obese-mice\nThe combined metabolite changed feeding in a specific mouse model.\nInjected Lac-Phe at 50 mg/kg reduced food intake in diet-induced obese mice; daily injection for ten days reduced intake, adiposity and weight.\nModel: Intraperitoneal pharmacological dosing in diet-induced obese mice.\nLimitations: Oral dosing did not reproduce the effect; lean mice did not respond even up to 150 mg/kg. Lactate and phenylalanine separately did not reproduce it.\nEvidence access: Primary full text\nAn exercise-inducible metabolite that suppresses feeding and obesity. · 2022 · https://pubmed.ncbi.nlm.nih.gov/35705806/ · DOI 10.1038/s41586-022-04828-5","model_system":"Intraperitoneal pharmacological dosing in diet-induced obese mice.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"54de7c56-1e60-5d7b-8ef9-852c58ae92ba","stable_key":"import-56f1d914-e7da-595a-af69-c217b2b47407","title":"L-Phenylalanine: transport, protein synthesis, cofactor recycling and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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