{"id":"d602a07b-6427-5f1b-9b5e-67998ae13f97","stable_key":"e69ef6a0-0e23-5eac-aca1-cf333781b962:ino-gpi-initiation","predicate":"produces","statement":"GPI N-acetylglucosaminyltransferase initiates anchor biosynthesis by transferring GlcNAc from UDP-GlcNAc to PI.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"ed224ed6-8726-5259-b30d-522af7d2ecf1","mechanism_event_label":"Inositol-containing membrane lipid is a starting material for anchors that hold proteins at the cell surface.","subject":{"id":"9d901059-e0d6-5670-a99b-5276cd5c7b64","slug":"gpi-gnt-complex","display_name":"Human GPI N-acetylglucosaminyltransferase complex","entity_type_key":"protein_complex"},"object":{"id":"77f589f0-94d1-5e2f-a16c-d48472eadfbc","slug":"glcnac-phosphatidylinositol","display_name":"N-acetylglucosaminyl-phosphatidylinositol (GlcNAc-PI)","entity_type_key":"lipid"},"evidence_count":1,"mechanism_event":{"id":"ed224ed6-8726-5259-b30d-522af7d2ecf1","stable_key":"e69ef6a0-0e23-5eac-aca1-cf333781b962:ino-gpi-initiation-event","event_type":"biochemical_relationship","label":"Inositol-containing membrane lipid is a starting material for anchors that hold proteins at the cell surface.","description":"GPI N-acetylglucosaminyltransferase initiates anchor biosynthesis by transferring GlcNAc from UDP-GlcNAc to PI.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"ae758982-4634-5f3a-b9c7-dbc58b744484","slug":"phosphatidylinositol","display_name":"Phosphatidylinositol (PI)","entity_type_key":"lipid"},"role":"substrate","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"cb1a9cff-8337-592c-8428-2e8a8713a4b0","slug":"udp-glcnac","display_name":"UDP-N-acetylglucosamine","entity_type_key":"small_molecule"},"role":"donor","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"6324c0ba-a7b5-55a8-9396-bb31a3fba19e","slug":"gpi-anchor-biosynthesis","display_name":"Glycosylphosphatidylinositol anchor biosynthesis","entity_type_key":"cellular_process"},"role":"pathway","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"9d901059-e0d6-5670-a99b-5276cd5c7b64","slug":"gpi-gnt-complex","display_name":"Human GPI N-acetylglucosaminyltransferase complex","entity_type_key":"protein_complex"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"77f589f0-94d1-5e2f-a16c-d48472eadfbc","slug":"glcnac-phosphatidylinositol","display_name":"N-acetylglucosaminyl-phosphatidylinositol (GlcNAc-PI)","entity_type_key":"lipid"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/inositol-research/40378954.fulltext.txt\", \"locator\": \"Exact primary full-text span; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"3cc2c3ec38a92b3f125ade893ea74380cd41dab129c560dcca339f6f9dae1ed8\", \"start_char\": 602, \"end_char\": 1102, \"text_sha256\": \"efb80e58245431f0e571ea2fbf380914ac09fe0c07c148fd9a062f5e744166e4\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human-cell gene perturbation and isolated-complex activity","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"ARV1/PIGQ association and PI utilization","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The ring-shaped assembly was AlphaFold-predicted, not an experimentally resolved structure. Effects on GPI-anchored proteins varied by cell and protein.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Inositol research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"inositol","display_name":"Inositol (stereoisomer family)","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Inositol-containing membrane lipid is a starting material for anchors that hold proteins at the cell surface.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[ino-p40378954] ARV1 is a component of the enzyme initiating glycosylphosphatidylinositol biosynthesis. (2025). https://pubmed.ncbi.nlm.nih.gov/40378954/ DOI: 10.1016/j.jbc.2025.110236","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Fibroblasts, HEK293 cells and ER enzyme complex","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"50f6df14-902b-54e2-9a2b-4b2aedb823bc","evidence_kind":"source_excerpt","locator":"Lines 1120-1131","start_line":1120,"end_line":1131,"excerpt":"### ino-gpi-initiation\nGPI N-acetylglucosaminyltransferase initiates anchor biosynthesis by transferring GlcNAc from UDP-GlcNAc to PI.\nCondition category: normal\nnutrient_topic: Inositol research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Inositol-containing membrane lipid is a starting material for anchors that hold proteins at the cell surface.\norganism: Homo sapiens\ntissue_or_cell_type: Fibroblasts, HEK293 cells and ER enzyme complex\nexperimental_model: Human-cell gene perturbation and isolated-complex activity\nlimitations: The ring-shaped assembly was AlphaFold-predicted, not an experimentally resolved structure. Effects on GPI-anchored proteins varied by cell and protein.\nexposure: ARV1/PIGQ association and PI utilization\nevidence_span: {\"source_cache\": \"artifacts/inositol-research/40378954.fulltext.txt\", \"locator\": \"Exact primary full-text span; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"3cc2c3ec38a92b3f125ade893ea74380cd41dab129c560dcca339f6f9dae1ed8\", \"start_char\": 602, \"end_char\": 1102, \"text_sha256\": \"efb80e58245431f0e571ea2fbf380914ac09fe0c07c148fd9a062f5e744166e4\"}\n[ino-p40378954] ARV1 is a component of the enzyme initiating glycosylphosphatidylinositol biosynthesis. (2025). https://pubmed.ncbi.nlm.nih.gov/40378954/ DOI: 10.1016/j.jbc.2025.110236","model_system":"Human-cell gene perturbation and isolated-complex activity","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [ino-p40378954] ARV1 is a component of the enzyme initiating glycosylphosphatidylinositol biosynthesis. (2025). https://pubmed.ncbi.nlm.nih.gov/40378954/ DOI: 10.1016/j.jbc.2025.110236","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"d590c659-b774-50e6-9f1d-aff61d5f7c9c","stable_key":"import-e69ef6a0-0e23-5eac-aca1-cf333781b962","title":"Inositol: synthesis, signaling, mineral interactions and conditional deficiency (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"f7adbcedad647d5f8a3cc9e46bfa515e83c508be9c2a4e9d59c5f5dfb0667d53","revision_id":"a82d187c-60fb-52d1-8482-eadb92860f33","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}