{"id":"d5be01c4-b820-518f-bd80-40db1a77b389","stable_key":"88eb7407-f147-558b-889b-0c89c4e0aa4a:spermidine-heart-atg5","predicate":"deletion_prevents","statement":"Cardiomyocyte-specific Atg5 loss abolished the reported cardioprotective response to spermidine.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"7225c66f-93ec-55f3-8c5f-70d190c36f6b","mechanism_event_label":"The recycling machinery was required for this benefit.","subject":{"id":"d876e242-37d5-5468-9b82-39cd283aad4b","slug":"mouse-atg5","display_name":"Mouse autophagy related 5 / Atg5","entity_type_key":"protein"},"object":{"id":"bf49cc91-b581-5a23-bef2-c981aff27fa4","slug":"mouse-spermidine-cardiac-response","display_name":"Mouse spermidine-associated cardiac protection","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"7225c66f-93ec-55f3-8c5f-70d190c36f6b","stable_key":"88eb7407-f147-558b-889b-0c89c4e0aa4a:spermidine-heart-atg5-event","event_type":"observed_relationship","label":"The recycling machinery was required for this benefit.","description":"Cardiomyocyte-specific Atg5 loss abolished the reported cardioprotective response to spermidine.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"d876e242-37d5-5468-9b82-39cd283aad4b","slug":"mouse-atg5","display_name":"Mouse autophagy related 5 / Atg5","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"bf49cc91-b581-5a23-bef2-c981aff27fa4","slug":"mouse-spermidine-cardiac-response","display_name":"Mouse spermidine-associated cardiac protection","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"7014993c-468f-5c6b-ab04-80c87f9dfd9f","slug":"spermidine","display_name":"Spermidine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Genetically modified mice.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"A required pathway is not proof that autophagy alone explains all cardiac effects.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Spermidine collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"spermidine","display_name":"Spermidine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The recycling machinery was required for this benefit.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Cardioprotection and lifespan extension by the natural polyamine spermidine. · 2016 · https://pubmed.ncbi.nlm.nih.gov/27841876/ · DOI 10.1038/nm.4222","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"53e233ec-3466-570d-9b0f-04b93d308abb","evidence_kind":"source_excerpt","locator":"Lines 334-340","start_line":334,"end_line":340,"excerpt":"## spermidine-heart-atg5\nThe recycling machinery was required for this benefit.\nCardiomyocyte-specific Atg5 loss abolished the reported cardioprotective response to spermidine.\nModel: Genetically modified mice.\nLimitations: A required pathway is not proof that autophagy alone explains all cardiac effects.\nEvidence access: Primary abstract\nCardioprotection and lifespan extension by the natural polyamine spermidine. · 2016 · https://pubmed.ncbi.nlm.nih.gov/27841876/ · DOI 10.1038/nm.4222","model_system":"Genetically modified mice.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"4348f3f8-0180-58a3-b61c-55d03bb322d2","stable_key":"import-88eb7407-f147-558b-889b-0c89c4e0aa4a","title":"Spermidine: biosynthesis, hypusination, transport and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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