{"id":"d5a5565f-426c-5f72-9488-52013a2977d6","stable_key":"cb568d28-484a-5c2e-9fcc-2d780358e514:vc-transport-diet-brain-svct2","predicate":"deficiency-does-not-detectably-change","statement":"Dietary vitamin C restriction did not significantly alter SVCT2 mRNA or protein in guinea pig hippocampus, cerebellum or frontal cortex at day 70.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"266e40c1-9c09-5ba2-a0a2-66de41fc929b","mechanism_event_label":"The measured brain regions did not compensate by detectably increasing SVCT2 abundance.","subject":{"id":"da1a7137-08f2-5a36-8bb6-377fbd32e327","slug":"vitamin-c","display_name":"Vitamin C","entity_type_key":"chemical_species"},"object":{"id":"c5e5528c-79d7-50f5-bb58-5c1a48529751","slug":"brain-svct2-expression","display_name":"Brain SVCT2 expression","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"266e40c1-9c09-5ba2-a0a2-66de41fc929b","stable_key":"cb568d28-484a-5c2e-9fcc-2d780358e514:vc-transport-diet-brain-svct2-event","event_type":"observed_intervention","label":"The measured brain regions did not compensate by detectably increasing SVCT2 abundance.","description":"Dietary vitamin C restriction did not significantly alter SVCT2 mRNA or protein in guinea pig hippocampus, cerebellum or frontal cortex at day 70.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"e59c2002-b3e1-58a0-aa9f-1f6db895ffe9","slug":"guinea-pig-slc23a2","display_name":"Guinea pig sodium-dependent vitamin C transporter 2 / Slc23a2","entity_type_key":"protein"},"role":"measured transporter","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"da1a7137-08f2-5a36-8bb6-377fbd32e327","slug":"vitamin-c","display_name":"Vitamin C","entity_type_key":"chemical_species"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"c5e5528c-79d7-50f5-bb58-5c1a48529751","slug":"brain-svct2-expression","display_name":"Brain SVCT2 expression","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"cross_nutrient","value_text":"false","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Thirty female guinea pig offspring, control/deficient/repleted diets, postnatal day 70","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Prenatal/postnatal 100/100 mg vitamin C/kg diet versus 900/750 mg/kg controls; female offspring at day 70","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"No detected expression difference is not proof of unchanged transport flux, earlier expression, or every cell subtype.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin C research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-c","display_name":"Vitamin C","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Cavia porcellus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The measured brain regions did not compensate by detectably increasing SVCT2 abundance.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[brain2014] Chronic vitamin C deficiency promotes redox imbalance in the brain but does not alter sodium-dependent vitamin C transporter 2 expression. (2014). https://pubmed.ncbi.nlm.nih.gov/24787032/ DOI: 10.3390/nu6051809","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Hippocampus, cerebellum and frontal cortex","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"65ee741d-7829-563a-b316-e646e667ca29","evidence_kind":"source_excerpt","locator":"Lines 481-492","start_line":481,"end_line":492,"excerpt":"### vc-transport-diet-brain-svct2\nDietary vitamin C restriction did not significantly alter SVCT2 mRNA or protein in guinea pig hippocampus, cerebellum or frontal cortex at day 70.\nCondition category: nutrient_deficiency\nnutrient_topic: Vitamin C research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The measured brain regions did not compensate by detectably increasing SVCT2 abundance.\norganism: Cavia porcellus\ntissue_or_cell_type: Hippocampus, cerebellum and frontal cortex\nexperimental_model: Thirty female guinea pig offspring, control/deficient/repleted diets, postnatal day 70\nlimitations: No detected expression difference is not proof of unchanged transport flux, earlier expression, or every cell subtype.\nexposure: Prenatal/postnatal 100/100 mg vitamin C/kg diet versus 900/750 mg/kg controls; female offspring at day 70\ncross_nutrient: false\n[brain2014] Chronic vitamin C deficiency promotes redox imbalance in the brain but does not alter sodium-dependent vitamin C transporter 2 expression. (2014). https://pubmed.ncbi.nlm.nih.gov/24787032/ DOI: 10.3390/nu6051809","model_system":"Thirty female guinea pig offspring, control/deficient/repleted diets, postnatal day 70","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [brain2014] Chronic vitamin C deficiency promotes redox imbalance in the brain but does not alter sodium-dependent vitamin C transporter 2 expression. (2014). https://pubmed.ncbi.nlm.nih.gov/24787032/ DOI: 10.3390/nu6051809","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"fa811221-13bd-5c10-adc1-eaf097c7703c","stable_key":"import-cb568d28-484a-5c2e-9fcc-2d780358e514","title":"Vitamin C: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"b7fd83f956abb81855f2ea23199ba14e465cd91f4a2ac560277ec21ddafd7bfc","revision_id":"6cde9bbb-d712-5746-82a6-85b144253efa","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}