{"id":"d56f1be6-d8ab-5171-9fbb-ba2f6639f7b5","stable_key":"2f309d3a-d252-5dfb-ae24-993a625f1b6c:eugenol-platelet-agonist-selectivity","predicate":"selectively_inhibits","statement":"At 2 micromolar, eugenol inhibited collagen- and arachidonic-acid-induced aggregation in human platelets but did not inhibit thrombin- or U46619-induced aggregation.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"208b1874-fc14-54c3-978d-64feb766f41e","mechanism_event_label":"At 2 micromolar, eugenol inhibited collagen- and arachidonic-acid-induced aggregation in human platelets but did not inhibit thrombin- or U46619-induced aggregation.","subject":{"id":"4bea4ede-273a-5d8f-a4e7-e928156d2d82","slug":"eugenol","display_name":"Eugenol","entity_type_key":"small_molecule"},"object":{"id":"aff915e8-1d5a-5020-8818-6122ce0996a7","slug":"human-collagen-aa-platelet-aggregation","display_name":"Collagen- and arachidonic-acid-induced human platelet aggregation","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"208b1874-fc14-54c3-978d-64feb766f41e","stable_key":"2f309d3a-d252-5dfb-ae24-993a625f1b6c:eugenol-platelet-agonist-selectivity-event","event_type":"observed_relationship","label":"At 2 micromolar, eugenol inhibited collagen- and arachidonic-acid-induced aggregation in human platelets but did not inhibit thrombin- or U46619-induced aggregation.","description":"At 2 micromolar, eugenol inhibited collagen- and arachidonic-acid-induced aggregation in human platelets but did not inhibit thrombin- or U46619-induced aggregation.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"4bea4ede-273a-5d8f-a4e7-e928156d2d82","slug":"eugenol","display_name":"Eugenol","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"aff915e8-1d5a-5020-8818-6122ce0996a7","slug":"human-collagen-aa-platelet-aggregation","display_name":"Collagen- and arachidonic-acid-induced human platelet aggregation","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"15408bf1-872e-56f3-8a8d-b512624ecec0","slug":"collagen","display_name":"Collagen","entity_type_key":"protein_set"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"39f9446f-52a6-502d-b507-91137726f82c","slug":"arachidonic-acid","display_name":"AA","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"012f2392-e1c7-500a-a56b-1a4697e250c7","slug":"thrombin","display_name":"Thrombin","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"dose","value_text":"Eugenol 2 micromolar","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"Acute","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_scope","value_text":"literature_reviewed; model-specific source-derived curation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Washed human platelets; ADP-treated mouse pulmonary-thrombosis arm","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Ex-vivo platelet inhibition and mouse thrombosis do not establish oral antithrombotic efficacy or bleeding safety in humans.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Eugenol chapter; interacting nutrients, drugs, peptides and proteins retain their experimental settings.","comparator":null,"unit":null,"notes":"","entity":{"slug":"eugenol","display_name":"Eugenol","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Washed human platelets; ADP-treated mouse pulmonary-thrombosis arm","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"At 2 micromolar, eugenol inhibited collagen- and arachidonic-acid-induced aggregation in human platelets but did not inhibit thrombin- or U46619-induced aggregation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Eugenol Suppresses Platelet Activation and Mitigates Pulmonary Thromboembolism in Humans and Murine Models. (2024). https://pubmed.ncbi.nlm.nih.gov/38396774/ DOI: 10.3390/ijms25042098","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"In vitro; mouse intervention","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"Agonist-specific platelet aggregation","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"8361c86d-4f7c-5b4c-a5bc-1a0cb8e3e875","evidence_kind":"source_excerpt","locator":"Lines 11-20","start_line":11,"end_line":20,"excerpt":"## eugenol-platelet-agonist-selectivity\nAt 2 micromolar, eugenol inhibited collagen- and arachidonic-acid-induced aggregation in human platelets but did not inhibit thrombin- or U46619-induced aggregation.\nModel/species: Washed human platelets; ADP-treated mouse pulmonary-thrombosis arm\nTissue/system: Agonist-specific platelet aggregation\nExposure: Eugenol 2 micromolar\nRoute: In vitro; mouse intervention\nDuration: Acute\nLimits: Ex-vivo platelet inhibition and mouse thrombosis do not establish oral antithrombotic efficacy or bleeding safety in humans.\nPrimary reference: Eugenol Suppresses Platelet Activation and Mitigates Pulmonary Thromboembolism in Humans and Murine Models. (2024). https://pubmed.ncbi.nlm.nih.gov/38396774/ DOI: 10.3390/ijms25042098\nAccess: Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","model_system":"Washed human platelets; ADP-treated mouse pulmonary-thrombosis arm","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact original curation span with primary citation; not a publisher quotation.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"2f2ff5a4-7264-57fc-8368-46b9768ec668","stable_key":"import-2f309d3a-d252-5dfb-ae24-993a625f1b6c","title":"Eugenol: mechanism of action and interactions (2026-09-20)","document_type":"imported_text","citation_label":"Original AI-assisted source-specific curation with primary-study citations, model, exposure, route, duration, negative findings and limitations preserved. Not publisher full text.","file_path":"","sha256":"28c421c41a2fbfb00f3036084d43389247cfbfbad8f7549b7529fb4f0e84f61d","revision_id":"3377bcaa-7d21-522a-8571-50ff1977aba0","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}