{"id":"d5430bff-d506-5dd4-9d07-beb59a0b7841","stable_key":"434e1e27-eb90-583a-956b-62472726ffba:ca-camkii-autonomous-activity","predicate":"produces","statement":"Partial autophosphorylation generates CaMKII activity that persists in the absence of calcium in the purified assay.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"f3d25192-59e4-55c4-b5ae-060a4e266859","mechanism_event_label":"After activation, CaMKII can retain activity beyond the calcium pulse.","subject":{"id":"c91a8ec8-1efa-5253-bb4c-435b8575f1cd","slug":"camkii","display_name":"Ca2+/calmodulin-dependent protein kinase II family","entity_type_key":"protein_family"},"object":{"id":"fa849354-deb0-5326-b07b-8ff0b67101c2","slug":"autophosphorylated-camkii","display_name":"Autophosphorylated CaMKII with autonomous activity","entity_type_key":"protein_state"},"evidence_count":1,"mechanism_event":{"id":"f3d25192-59e4-55c4-b5ae-060a4e266859","stable_key":"434e1e27-eb90-583a-956b-62472726ffba:ca-camkii-autonomous-activity-event","event_type":"protein_modification","label":"After activation, CaMKII can retain activity beyond the calcium pulse.","description":"Partial autophosphorylation generates CaMKII activity that persists in the absence of calcium in the purified assay.","status":"provisional","compartment":{"slug":"cytosol","display_name":"Cytosol"},"participants":[{"entity":{"id":"c91a8ec8-1efa-5253-bb4c-435b8575f1cd","slug":"camkii","display_name":"Ca2+/calmodulin-dependent protein kinase II family","entity_type_key":"protein_family"},"role":"kinase","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"fa849354-deb0-5326-b07b-8ff0b67101c2","slug":"autophosphorylated-camkii","display_name":"Autophosphorylated CaMKII with autonomous activity","entity_type_key":"protein_state"},"role":"product_state","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"58b974f1-d389-5bf6-81cd-889c44442c42","slug":"atp","display_name":"ATP","entity_type_key":"small_molecule"},"role":"phosphoryl_donor","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"bc9ed3a1-38d5-5bc3-b2a5-9f9f7633660b","slug":"cytosol","display_name":"Cytosol","entity_type_key":"organelle"},"role":"experimental_location","stoichiometry":null,"state_label":"","sequence_order":3,"notes":"Study location; presence here is not a separate transport or causal claim."}]},"contexts":[{"dimension":"compartment_description","value_text":"Cytosolic kinase complex","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Purified mammalian brain CaMKII; phosphorylation and substrate-kinase assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Autonomy depends on phosphorylation conditions; not all self-phosphorylation activates and persistence in living cells is not quantified.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Calcium research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"calcium","display_name":"Calcium","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Mammalia","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"After activation, CaMKII can retain activity beyond the calcium pulse.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[ca-miller1986] Regulation of brain type II Ca2+/calmodulin-dependent protein kinase by autophosphorylation: a Ca2+-triggered molecular switch (1986). https://pubmed.ncbi.nlm.nih.gov/3006921/ DOI: 10.1016/0092-8674(86)90008-5","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"research_relationship_category","value_text":"protein_modification","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Brain enzyme preparation","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"7a52fa2f-e3ac-5a01-a717-19085197743a","evidence_kind":"source_excerpt","locator":"Lines 451-462","start_line":451,"end_line":462,"excerpt":"### ca-camkii-autonomous-activity\nPartial autophosphorylation generates CaMKII activity that persists in the absence of calcium in the purified assay.\nCondition category: normal\nnutrient_topic: Calcium research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: After activation, CaMKII can retain activity beyond the calcium pulse.\norganism: Mammalia\ntissue_or_cell_type: Brain enzyme preparation\nexperimental_model: Purified mammalian brain CaMKII; phosphorylation and substrate-kinase assays\nlimitations: Autonomy depends on phosphorylation conditions; not all self-phosphorylation activates and persistence in living cells is not quantified.\nresearch_relationship_category: protein_modification\ncompartment_description: Cytosolic kinase complex\n[ca-miller1986] Regulation of brain type II Ca2+/calmodulin-dependent protein kinase by autophosphorylation: a Ca2+-triggered molecular switch (1986). https://pubmed.ncbi.nlm.nih.gov/3006921/ DOI: 10.1016/0092-8674(86)90008-5","model_system":"Purified mammalian brain CaMKII; phosphorylation and substrate-kinase assays","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [ca-miller1986] Regulation of brain type II Ca2+/calmodulin-dependent protein kinase by autophosphorylation: a Ca2+-triggered molecular switch (1986). https://pubmed.ncbi.nlm.nih.gov/3006921/ DOI: 10.1016/0092-8674(86)90008-5","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"3c083fa0-59c6-50e9-af42-678d0e3c1006","stable_key":"import-434e1e27-eb90-583a-956b-62472726ffba","title":"Calcium: mechanism-first literature curation (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"965c934d6b147f2b62f8d45ce6d7a87681a600bfe5c46a585b4704889afd3fc1","revision_id":"543ec48b-4f70-5197-9d76-f5a3c443664c","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}