{"id":"d529d01a-aaaa-5ebf-846e-b5782186c3ea","stable_key":"ec174d5a-4903-5745-8646-df0e9d4265e8:rat-mthfd2l-phosphate-nadp-inhibition","predicate":"inhibits","statement":"Inorganic phosphate inhibits the NADP+-dependent dehydrogenase activity of rat MTHFD2L.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"92575c74-4287-5ff5-8cf0-6562ab03f202","mechanism_event_label":"The same phosphate has the opposite effect with the alternative electron carrier.","subject":{"id":"ee63fe8e-92f3-552f-8049-a5f1fa1e1523","slug":"phosphate-ion","display_name":"Inorganic phosphate (Pi; protonation depends on pH)","entity_type_key":"ion"},"object":{"id":"ab8012eb-70cc-5a65-b8d6-2fb31a7141f5","slug":"rat-mthfd2l","display_name":"Rat mitochondrial MTHFD2L","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"92575c74-4287-5ff5-8cf0-6562ab03f202","stable_key":"ec174d5a-4903-5745-8646-df0e9d4265e8:rat-mthfd2l-phosphate-nadp-inhibition-event","event_type":"biochemical_relationship","label":"The same phosphate has the opposite effect with the alternative electron carrier.","description":"Inorganic phosphate inhibits the NADP+-dependent dehydrogenase activity of rat MTHFD2L.","status":"provisional","compartment":{"slug":"mitochondrial-matrix","display_name":"Mitochondrial matrix"},"participants":[{"entity":{"id":"ee63fe8e-92f3-552f-8049-a5f1fa1e1523","slug":"phosphate-ion","display_name":"Inorganic phosphate (Pi; 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topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"folate","display_name":"Folate (vitamin B9)","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Rattus norvegicus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The same phosphate has the opposite effect with the alternative electron carrier.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[shin-2014] Mitochondrial MTHFD2L is a dual redox cofactor-specific methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase expressed in both adult and embryonic tissues (2014). https://pubmed.ncbi.nlm.nih.gov/24733394/ DOI: 10.1074/jbc.m114.555573","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Cell-free","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"31a26c91-617d-5b7f-8df3-19b636167d44","evidence_kind":"source_excerpt","locator":"Lines 1334-1345","start_line":1334,"end_line":1345,"excerpt":"### rat-mthfd2l-phosphate-nadp-inhibition\nInorganic phosphate inhibits the NADP+-dependent dehydrogenase activity of rat MTHFD2L.\nCondition category: normal\nnutrient_topic: Folate and folic acid research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The same phosphate has the opposite effect with the alternative electron carrier.\norganism: Rattus norvegicus\ntissue_or_cell_type: Cell-free\nexperimental_model: Cofactor omission/addition assays\nlimitations: No dietary magnesium threshold follows from these assays.\nexposure: Assay conditions described in the linked primary study.\ncross_nutrient: Direct magnesium/phosphate requirement in folate oxidation.\n[shin-2014] Mitochondrial MTHFD2L is a dual redox cofactor-specific methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase expressed in both adult and embryonic tissues (2014). https://pubmed.ncbi.nlm.nih.gov/24733394/ DOI: 10.1074/jbc.m114.555573","model_system":"Cofactor omission/addition assays","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [shin-2014] Mitochondrial MTHFD2L is a dual redox cofactor-specific methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase expressed in both adult and embryonic tissues (2014). https://pubmed.ncbi.nlm.nih.gov/24733394/ DOI: 10.1074/jbc.m114.555573","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"f4ce1a62-9582-5f7a-84f5-a23d0e1bfc68","stable_key":"import-ec174d5a-4903-5745-8646-df0e9d4265e8","title":"Folate and folic acid: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"e564d43989ece1006c95cd0748e9af6fe369074599a2eebba0a99ebff864b0dd","revision_id":"76674a33-b2a1-5e41-b71b-44399038ff7c","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}