{"id":"d5022c1d-fb42-5d60-a9da-0e876d71ca9e","stable_key":"cfa4bd86-d88d-555a-975c-68877bf00e0f:lutein-mouse-rpe65-isomerase","predicate":"catalyzes_formation_of","statement":"Mouse RPE65 supported lutein isomerization in cultured cells despite mice not normally concentrating meso-zeaxanthin in the eye.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"8a9e24cc-3ee6-561d-aca0-53d7fefa12d9","mechanism_event_label":"Enzyme capacity and whole-tissue pigment accumulation are different.","subject":{"id":"72b97c48-d00b-5086-bb8c-ff35c5177e24","slug":"mouse-rpe65","display_name":"Mouse RPE65","entity_type_key":"protein"},"object":{"id":"24cea833-2dbe-5746-be4d-06b3b7c6315c","slug":"meso-zeaxanthin","display_name":"meso-Zeaxanthin / (3R,3-prime-S)-zeaxanthin","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"8a9e24cc-3ee6-561d-aca0-53d7fefa12d9","stable_key":"cfa4bd86-d88d-555a-975c-68877bf00e0f:lutein-mouse-rpe65-isomerase-event","event_type":"biochemical_relationship","label":"Enzyme capacity and whole-tissue pigment accumulation are different.","description":"Mouse RPE65 supported lutein isomerization in cultured cells despite mice not normally concentrating meso-zeaxanthin in the eye.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"5dd84367-3f8e-5a3f-b952-a96174ea51fa","slug":"lutein","display_name":"Lutein","entity_type_key":"small_molecule"},"role":"substrate","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"72b97c48-d00b-5086-bb8c-ff35c5177e24","slug":"mouse-rpe65","display_name":"Mouse RPE65","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"24cea833-2dbe-5746-be4d-06b3b7c6315c","slug":"meso-zeaxanthin","display_name":"meso-Zeaxanthin / (3R,3-prime-S)-zeaxanthin","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/lutein-research/41415389.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"b4eb5b9290310947be962df7dae42190e5afa6ffd726e869cb842d7f7ed15a41\", \"start_char\": 0, \"end_char\": 1304, \"text_sha256\": \"b4eb5b9290310947be962df7dae42190e5afa6ffd726e869cb842d7f7ed15a41\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Cultured-cell enzyme expression, mutagenesis and structural modeling","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Lutein substrate; E148Q substitution; binding-protein coexpression","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Proposed radical transition state remains a mechanistic interpretation, not directly observed chemistry.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Lutein research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"lutein","display_name":"Lutein","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human, chicken and mouse RPE65","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Enzyme capacity and whole-tissue pigment accumulation are different.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[lutein-p41415389] Mechanism of Lutein to meso-Zeaxanthin Isomerization by RPE65 Catalysis. (2025). https://pubmed.ncbi.nlm.nih.gov/41415389/ DOI: 10.64898/2025.12.10.693550","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Cultured cells and molecular models","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"5254806f-5241-5d9e-9c3e-d94f231cea78","evidence_kind":"source_excerpt","locator":"Lines 190-201","start_line":190,"end_line":201,"excerpt":"### lutein-mouse-rpe65-isomerase\nMouse RPE65 supported lutein isomerization in cultured cells despite mice not normally concentrating meso-zeaxanthin in the eye.\nCondition category: normal\nnutrient_topic: Lutein research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Enzyme capacity and whole-tissue pigment accumulation are different.\norganism: Human, chicken and mouse RPE65\ntissue_or_cell_type: Cultured cells and molecular models\nexperimental_model: Cultured-cell enzyme expression, mutagenesis and structural modeling\nlimitations: Proposed radical transition state remains a mechanistic interpretation, not directly observed chemistry.\nexposure: Lutein substrate; E148Q substitution; binding-protein coexpression\nevidence_span: {\"source_cache\": \"artifacts/lutein-research/41415389.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"b4eb5b9290310947be962df7dae42190e5afa6ffd726e869cb842d7f7ed15a41\", \"start_char\": 0, \"end_char\": 1304, \"text_sha256\": \"b4eb5b9290310947be962df7dae42190e5afa6ffd726e869cb842d7f7ed15a41\"}\n[lutein-p41415389] Mechanism of Lutein to meso-Zeaxanthin Isomerization by RPE65 Catalysis. 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