{"id":"d4d4fa29-d120-57ed-9f2c-a90d21695167","stable_key":"507ed066-fd6d-5722-9223-97b66302e7e3:glycine-isovaleryl-glycine-limit","predicate":"does_not_increase_tested","statement":"Increasing glycine did not increase N-isovalerylglycine formation in the reported in-vitro conjugation experiments.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"99fcae2e-05ee-5767-8255-f9b77ca09eb1","mechanism_event_label":"Adding substrate did not keep increasing this disposal reaction.","subject":{"id":"2b507258-430c-51fe-9fd2-e510c2c197a9","slug":"glycine","display_name":"Glycine","entity_type_key":"small_molecule"},"object":{"id":"3ac8c617-7037-540d-8eca-ce442f2036c2","slug":"isovalerylglycine","display_name":"N-Isovalerylglycine","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"99fcae2e-05ee-5767-8255-f9b77ca09eb1","stable_key":"507ed066-fd6d-5722-9223-97b66302e7e3:glycine-isovaleryl-glycine-limit-event","event_type":"observed_relationship","label":"Adding substrate did not keep increasing this disposal reaction.","description":"Increasing glycine did not increase N-isovalerylglycine formation in the reported in-vitro conjugation experiments.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"2b507258-430c-51fe-9fd2-e510c2c197a9","slug":"glycine","display_name":"Glycine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"3ac8c617-7037-540d-8eca-ce442f2036c2","slug":"isovalerylglycine","display_name":"N-Isovalerylglycine","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"3febb8ae-e5ee-51ef-912f-826f4e2e3ddd","slug":"glyat","display_name":"Human glycine N-acyltransferase / GLYAT","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"12170152-70f0-54b8-9ca7-3ed6dd9b8e7f","slug":"glyatl1","display_name":"Human glycine N-acyltransferase-like 1 / GLYATL1","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"bb6288c5-83e5-5cbc-9e5f-b6c74eed8b8f","slug":"isovaleryl-coa","display_name":"Isovaleryl-CoA","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"GLYAT/GLYATL1 enzyme experiments.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Not evidence that glycine treatment never works in isovaleric acidemia; substrate, enzyme and kinetic context matter.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Glycine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"glycine","display_name":"Glycine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Adding substrate did not keep increasing this disposal reaction.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"The glycine N-acyltransferases, GLYAT and GLYATL1, contribute to the detoxification of isovaleryl-CoA - an in-silico and in vitro validation. · 2023 · https://pubmed.ncbi.nlm.nih.gov/36817957/ · DOI 10.1016/j.csbj.2023.01.041","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"87928756-4ae2-5536-b5a8-cf20210c37db","evidence_kind":"source_excerpt","locator":"Lines 306-312","start_line":306,"end_line":312,"excerpt":"## glycine-isovaleryl-glycine-limit\nAdding substrate did not keep increasing this disposal reaction.\nIncreasing glycine did not increase N-isovalerylglycine formation in the reported in-vitro conjugation experiments.\nModel: GLYAT/GLYATL1 enzyme experiments.\nLimitations: Not evidence that glycine treatment never works in isovaleric acidemia; substrate, enzyme and kinetic context matter.\nEvidence access: Primary full text\nThe glycine N-acyltransferases, GLYAT and GLYATL1, contribute to the detoxification of isovaleryl-CoA - an in-silico and in vitro validation. · 2023 · https://pubmed.ncbi.nlm.nih.gov/36817957/ · DOI 10.1016/j.csbj.2023.01.041","model_system":"GLYAT/GLYATL1 enzyme experiments.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"7b2859d8-e2bf-51e7-8e32-0715c10cbc49","stable_key":"import-507ed066-fd6d-5722-9223-97b66302e7e3","title":"Glycine: supply, one-carbon allocation, receptors and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"4fabd2fc8e71ca156c91023e7340be3f5a02ae03e91d3a24b231c2adfb0de0aa","revision_id":"24801e49-34a8-53c3-bdf5-b59143441450","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}