{"id":"d4b07420-b993-501d-979f-05b2ec8e00b5","stable_key":"f992b796-377d-53bf-a39b-7e5d41dbe194:b5-met-diet-hci002-acetylcoa","predicate":"dietary_deprivation_reduces","statement":"Pantothenate deprivation reduced the amount of glucose-derived carbon-13-labeled acetyl-CoA in HCI002 xenografts.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"cc0e2758-43b6-5d69-8642-7ae9f09b5d6a","mechanism_event_label":"B5 restriction also reduced the measured labeled acetyl-CoA formed from glucose in these tumors.","subject":{"id":"7bc62072-9517-5563-a3b8-8c5ab64a464b","slug":"pantothenate","display_name":"Pantothenate (vitamin B5)","entity_type_key":"small_molecule"},"object":{"id":"b7ed6e4c-e560-5cee-b68f-895f21862e6f","slug":"acetyl-coa","display_name":"Acetyl-CoA","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"cc0e2758-43b6-5d69-8642-7ae9f09b5d6a","stable_key":"f992b796-377d-53bf-a39b-7e5d41dbe194:b5-met-diet-hci002-acetylcoa-event","event_type":"biochemical_relationship","label":"B5 restriction also reduced the measured labeled acetyl-CoA formed from glucose in these tumors.","description":"Pantothenate deprivation reduced the amount of glucose-derived carbon-13-labeled acetyl-CoA in HCI002 xenografts.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"8ae7848b-f172-5e09-8acf-5ca914907b0b","slug":"glucose","display_name":"D-glucose","entity_type_key":"small_molecule"},"role":"isotope_labeled_carbon_source","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"4faa6456-aff8-59eb-9e3f-3326a436e401","slug":"coenzyme-a","display_name":"Coenzyme A","entity_type_key":"small_molecule"},"role":"acetyl_carrier","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"7bc62072-9517-5563-a3b8-8c5ab64a464b","slug":"pantothenate","display_name":"Pantothenate (vitamin B5)","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"b7ed6e4c-e560-5cee-b68f-895f21862e6f","slug":"acetyl-coa","display_name":"Acetyl-CoA","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"cross_nutrient","value_text":"true","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_location","value_text":"Full text lines 107, 123–125; Fig. 4F–G; Extended Data Fig. 5G–H","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Matched PA-free/control diets in NOD/Scid mice bearing orthotopic human HCI002 breast-cancer xenografts with carbon-13 glucose tracing","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Diets introduced at 6 weeks of age, progressively over 4 days; typically 5 weeks on the assigned diet before implantation and continued until collection. PA absence verified by LC-MS; control was composition-matched with PA added. Control n=7, PA-free n=8 tumors from independent mice. Carbon-13 glucose tracing followed by LC-MS.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The same paper found no significant fall in the corresponding CoA/acetyl-CoA pools in WMmix tumors. Tumor concentration and tracer labeling do not establish universal CoA depletion or a human anticancer diet; cellular composition and pool size can affect the measured label. This experiment does not establish a dietary pantothenate threshold or benefit from B5 supplementation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Pantothenic acid (vitamin B5) research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"pantothenate","display_name":"Pantothenate (vitamin B5)","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human HCI002 grafts in Mus musculus hosts","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"B5 restriction also reduced the measured labeled acetyl-CoA formed from glucose in these tumors.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[b5-met-myc2024] Vitamin B<sub>5</sub> supports MYC oncogenic metabolism and tumor progression in breast cancer. (2023). https://pubmed.ncbi.nlm.nih.gov/37946084/ DOI: 10.1038/s42255-023-00915-7","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Orthotopic HCI002 tumor tissue","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"96ba5cc2-0f6a-5743-a156-a512b89fb7f3","evidence_kind":"source_excerpt","locator":"Lines 1117-1129","start_line":1117,"end_line":1129,"excerpt":"### b5-met-diet-hci002-acetylcoa\nPantothenate deprivation reduced the amount of glucose-derived carbon-13-labeled acetyl-CoA in HCI002 xenografts.\nCondition category: nutrient_deficiency\nnutrient_topic: Pantothenic acid (vitamin B5) research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: B5 restriction also reduced the measured labeled acetyl-CoA formed from glucose in these tumors.\norganism: Human HCI002 grafts in Mus musculus hosts\ntissue_or_cell_type: Orthotopic HCI002 tumor tissue\nexperimental_model: Matched PA-free/control diets in NOD/Scid mice bearing orthotopic human HCI002 breast-cancer xenografts with carbon-13 glucose tracing\nlimitations: The same paper found no significant fall in the corresponding CoA/acetyl-CoA pools in WMmix tumors. Tumor concentration and tracer labeling do not establish universal CoA depletion or a human anticancer diet; cellular composition and pool size can affect the measured label. This experiment does not establish a dietary pantothenate threshold or benefit from B5 supplementation.\nexposure: Diets introduced at 6 weeks of age, progressively over 4 days; typically 5 weeks on the assigned diet before implantation and continued until collection. PA absence verified by LC-MS; control was composition-matched with PA added. Control n=7, PA-free n=8 tumors from independent mice. Carbon-13 glucose tracing followed by LC-MS.\ncross_nutrient: true\nevidence_location: Full text lines 107, 123–125; Fig. 4F–G; Extended Data Fig. 5G–H\n[b5-met-myc2024] Vitamin B<sub>5</sub> supports MYC oncogenic metabolism and tumor progression in breast cancer. (2023). https://pubmed.ncbi.nlm.nih.gov/37946084/ DOI: 10.1038/s42255-023-00915-7","model_system":"Matched PA-free/control diets in NOD/Scid mice bearing orthotopic human HCI002 breast-cancer xenografts with carbon-13 glucose tracing","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [b5-met-myc2024] Vitamin B<sub>5</sub> supports MYC oncogenic metabolism and tumor progression in breast cancer. 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