{"id":"d49afc48-27db-578c-9ecf-6a44f9e8940e","stable_key":"10aa417f-4b03-599f-a453-4aa09edeb27c:alanine-ampk-pools","predicate":"changes","statement":"Alanine increased the AMP/ATP ratio and glutamate while decreasing 2-oxoglutarate and other TCA intermediate pools in rat H4IIE cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"7b34d920-8ad2-534a-ad59-b6b7905fc521","mechanism_event_label":"Moving amino nitrogen changed the cell’s carbon pools and energy balance.","subject":{"id":"ebdc4461-c059-563e-88b0-422c21fdaa25","slug":"alanine","display_name":"L-Alanine","entity_type_key":"small_molecule"},"object":{"id":"f06dabf9-8dc8-550b-bfa5-c38f90b35804","slug":"rat-h4iie-amp-atp-ratio","display_name":"AMP/ATP ratio in rat H4IIE cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"7b34d920-8ad2-534a-ad59-b6b7905fc521","stable_key":"10aa417f-4b03-599f-a453-4aa09edeb27c:alanine-ampk-pools-event","event_type":"observed_relationship","label":"Moving amino nitrogen changed the cell’s carbon pools and energy balance.","description":"Alanine increased the AMP/ATP ratio and glutamate while decreasing 2-oxoglutarate and other TCA intermediate pools in rat H4IIE cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"ebdc4461-c059-563e-88b0-422c21fdaa25","slug":"alanine","display_name":"L-Alanine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"f06dabf9-8dc8-550b-bfa5-c38f90b35804","slug":"rat-h4iie-amp-atp-ratio","display_name":"AMP/ATP ratio in rat H4IIE cells","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"d47321ac-44bb-5a88-afdb-6435b57a91b9","slug":"amp","display_name":"AMP","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"58b974f1-d389-5bf6-81cd-889c44442c42","slug":"atp","display_name":"ATP","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"7da684a4-2641-5bc6-93ae-8c6aa384e487","slug":"glutamate","display_name":"L-Glutamate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"628ddc2d-e4d9-59c7-ae76-0c0cab80a6b1","slug":"2-oxoglutarate","display_name":"2-Oxoglutarate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Acute rat H4IIE metabolomics; AMP/ATP approximately 1.5-fold higher at 15 minutes.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Pool sizes are not flux measurements; increased ATP-consuming urea synthesis was proposed rather than directly proven.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Alanine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"alanine","display_name":"L-Alanine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Moving amino nitrogen changed the cell’s carbon pools and energy balance.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"l-Alanine activates hepatic AMP-activated protein kinase and modulates systemic glucose metabolism. · 2018 · https://pubmed.ncbi.nlm.nih.gov/30190193/ · DOI 10.1016/j.molmet.2018.08.002","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"c8aaaad1-da95-5f07-acfe-d415fd2bca21","evidence_kind":"source_excerpt","locator":"Lines 264-270","start_line":264,"end_line":270,"excerpt":"## alanine-ampk-pools\nMoving amino nitrogen changed the cell’s carbon pools and energy balance.\nAlanine increased the AMP/ATP ratio and glutamate while decreasing 2-oxoglutarate and other TCA intermediate pools in rat H4IIE cells.\nModel: Acute rat H4IIE metabolomics; AMP/ATP approximately 1.5-fold higher at 15 minutes.\nLimitations: Pool sizes are not flux measurements; increased ATP-consuming urea synthesis was proposed rather than directly proven.\nEvidence access: Primary full text\nl-Alanine activates hepatic AMP-activated protein kinase and modulates systemic glucose metabolism. · 2018 · https://pubmed.ncbi.nlm.nih.gov/30190193/ · DOI 10.1016/j.molmet.2018.08.002","model_system":"Acute rat H4IIE metabolomics; AMP/ATP approximately 1.5-fold higher at 15 minutes.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"01c5554c-a8ba-5c86-9b01-a0fa1a886cdb","stable_key":"import-10aa417f-4b03-599f-a453-4aa09edeb27c","title":"L-Alanine: carbon, nitrogen, protein synthesis and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; 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