{"id":"d422ecf4-1955-554c-b460-36cad0a4c26c","stable_key":"3b71c9a2-e2fe-5ab8-a85b-15ae1be836dd:silica-osteoclast-aqp9","predicate":"increases_transcript","statement":"AQP9 mRNA increased significantly at 72 hours during silicon exposure.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"a60acdac-794d-5113-9e09-6a2d10ed6392","mechanism_event_label":"Transporter expression changed even while differentiation markers fell.","subject":{"id":"1ff7d527-b221-5c48-b48d-a015f7aebe6e","slug":"osa-enriched-silicon-osteoclast-preparation","display_name":"Silicon preparation containing 75% orthosilicic acid","entity_type_key":"chemical_species"},"object":{"id":"9220a6e9-b494-54d7-8376-e75269ab9e07","slug":"aqp9","display_name":"Human aquaporin 9 / AQP9","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"a60acdac-794d-5113-9e09-6a2d10ed6392","stable_key":"3b71c9a2-e2fe-5ab8-a85b-15ae1be836dd:silica-osteoclast-aqp9-event","event_type":"observed_relationship","label":"Transporter expression changed even while differentiation markers fell.","description":"AQP9 mRNA increased significantly at 72 hours during silicon exposure.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"1ff7d527-b221-5c48-b48d-a015f7aebe6e","slug":"osa-enriched-silicon-osteoclast-preparation","display_name":"Silicon preparation containing 75% orthosilicic acid","entity_type_key":"chemical_species"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"9220a6e9-b494-54d7-8376-e75269ab9e07","slug":"aqp9","display_name":"Human aquaporin 9 / AQP9","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"11b1cdb5-1b10-586b-9917-b4da18ac072f","slug":"silica","display_name":"Silica and soluble silicon","entity_type_key":"chemical_species"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"6db3cbef-401d-5ef3-ab6f-e6949aab7b40","slug":"human-osa-osteoclast-differentiation","display_name":"Human osteoclast differentiation under silicon exposure","entity_type_key":"cellular_process"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human CD14+ osteoclast cultures.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"No AQP9 loss-of-function test demonstrated that it mediated the resorption effect.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Silica collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"silica","display_name":"Silica and soluble silicon","entity_type_key":"chemical_species"}},{"dimension":"plain_language","value_text":"Transporter expression changed even while differentiation markers fell.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Orthosilicic acid inhibits human osteoclast differentiation and bone resorption. · 2024 · https://pubmed.ncbi.nlm.nih.gov/39405281/ · DOI 10.1371/journal.pone.0312169","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"278535f0-685e-53ca-b67d-7a1aa6e8c0ec","evidence_kind":"source_excerpt","locator":"Lines 256-262","start_line":256,"end_line":262,"excerpt":"## silica-osteoclast-aqp9\nTransporter expression changed even while differentiation markers fell.\nAQP9 mRNA increased significantly at 72 hours during silicon exposure.\nModel: Human CD14+ osteoclast cultures.\nLimitations: No AQP9 loss-of-function test demonstrated that it mediated the resorption effect.\nEvidence access: Primary full text\nOrthosilicic acid inhibits human osteoclast differentiation and bone resorption. · 2024 · https://pubmed.ncbi.nlm.nih.gov/39405281/ · DOI 10.1371/journal.pone.0312169","model_system":"Human CD14+ osteoclast cultures.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"dc4b461e-dd46-57ab-8213-8aeb7ff9e8d0","stable_key":"import-3b71c9a2-e2fe-5ab8-a85b-15ae1be836dd","title":"Silica: soluble silicon, cellular transport and particle-specific mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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