{"id":"d3fafcda-186e-53d7-ac1c-b92f145dbb2c","stable_key":"5d8e27d8-6a74-5560-827f-3f90908bbc34:ala-iscu-isca2-reconstitution","predicate":"reconstitutes","statement":"[2Fe-2S]-loaded human ISCU and ISCA2 reconstituted catalytically active human LIAS in vitro.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"0422ee08-7806-5bd7-8189-2b586e0c4ba9","mechanism_event_label":"These carrier proteins can help rebuild active enzyme under assay conditions.","subject":{"id":"3474afb8-5d3d-5b1c-a8a6-a5450091b5c0","slug":"iscu","display_name":"Human ISCU","entity_type_key":"protein"},"object":{"id":"f9fc9417-ca4f-5e0c-b01d-60b337493d34","slug":"lias","display_name":"Lipoic acid synthetase / LIAS","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"0422ee08-7806-5bd7-8189-2b586e0c4ba9","stable_key":"5d8e27d8-6a74-5560-827f-3f90908bbc34:ala-iscu-isca2-reconstitution-event","event_type":"biochemical_relationship","label":"These carrier proteins can help rebuild active enzyme under assay conditions.","description":"[2Fe-2S]-loaded human ISCU and ISCA2 reconstituted catalytically active human LIAS in vitro.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"9db49c81-8504-51b8-95b8-8813f0d97497","slug":"isca2","display_name":"Human ISCA2","entity_type_key":"protein"},"role":"alternative_donor","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"02107865-f169-5624-9ea4-9448a4cce185","slug":"iron-sulfur-2fe2s","display_name":"[2Fe-2S] iron-sulfur cluster","entity_type_key":"chemical_species"},"role":"donor_cluster","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"3474afb8-5d3d-5b1c-a8a6-a5450091b5c0","slug":"iscu","display_name":"Human ISCU","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"f9fc9417-ca4f-5e0c-b01d-60b337493d34","slug":"lias","display_name":"Lipoic acid synthetase / LIAS","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/ala-research/33562493.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"4261f63098ce640cc87368d3f23f294441fdfa2a3f9b271086821fc1196bb3ac\", \"start_char\": 0, \"end_char\": 1544, \"text_sha256\": \"4261f63098ce640cc87368d3f23f294441fdfa2a3f9b271086821fc1196bb3ac\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Recombinant human LIAS cluster reconstitution, EPR and LC-MS","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"[2Fe-2S]-loaded ISCU or ISCA2 donors","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"In vitro donor capacity does not establish a unique physiological donor or exclude other routes.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Alpha-lipoic acid research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"lipoic-acid","display_name":"Lipoic acid","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human proteins","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"These carrier proteins can help rebuild active enzyme under assay conditions.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[ala-p33562493] Characterization and Reconstitution of Human Lipoyl Synthase (LIAS) Supports ISCA2 and ISCU as Primary Cluster Donors and an Ordered Mechanism of Cluster Assembly. (2021). https://pubmed.ncbi.nlm.nih.gov/33562493/ DOI: 10.3390/ijms22041598","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Two LIAS iron-sulfur sites","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"0f6fabc6-c839-5d7e-b738-2a9cff4bc13f","evidence_kind":"source_excerpt","locator":"Lines 390-401","start_line":390,"end_line":401,"excerpt":"### ala-iscu-isca2-reconstitution\n[2Fe-2S]-loaded human ISCU and ISCA2 reconstituted catalytically active human LIAS in vitro.\nCondition category: normal\nnutrient_topic: Alpha-lipoic acid research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: These carrier proteins can help rebuild active enzyme under assay conditions.\norganism: Human proteins\ntissue_or_cell_type: Two LIAS iron-sulfur sites\nexperimental_model: Recombinant human LIAS cluster reconstitution, EPR and LC-MS\nlimitations: In vitro donor capacity does not establish a unique physiological donor or exclude other routes.\nexposure: [2Fe-2S]-loaded ISCU or ISCA2 donors\nevidence_span: {\"source_cache\": \"artifacts/ala-research/33562493.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"4261f63098ce640cc87368d3f23f294441fdfa2a3f9b271086821fc1196bb3ac\", \"start_char\": 0, \"end_char\": 1544, \"text_sha256\": \"4261f63098ce640cc87368d3f23f294441fdfa2a3f9b271086821fc1196bb3ac\"}\n[ala-p33562493] Characterization and Reconstitution of Human Lipoyl Synthase (LIAS) Supports ISCA2 and ISCU as Primary Cluster Donors and an Ordered Mechanism of Cluster Assembly. (2021). https://pubmed.ncbi.nlm.nih.gov/33562493/ DOI: 10.3390/ijms22041598","model_system":"Recombinant human LIAS cluster reconstitution, EPR and LC-MS","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [ala-p33562493] Characterization and Reconstitution of Human Lipoyl Synthase (LIAS) Supports ISCA2 and ISCU as Primary Cluster Donors and an Ordered Mechanism of Cluster Assembly. (2021). https://pubmed.ncbi.nlm.nih.gov/33562493/ DOI: 10.3390/ijms22041598","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"d8afa8c2-ced9-5b28-90ca-2ac120ec7202","stable_key":"import-5d8e27d8-6a74-5560-827f-3f90908bbc34","title":"Alpha-lipoic acid: cofactor assembly, redox signaling and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"611da198ab85a272eb26b64ee330ef6d1a762853a8481f3191c4acfca9f3cf3d","revision_id":"618b4ce6-3157-5222-8325-408064be5ea5","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}