{"id":"d3ef065e-3295-52d4-af0f-2ddaf00a672d","stable_key":"b428117d-3a8c-5e74-8711-168153e19545:aspartame-calcium-activation","predicate":"enhances_tested","statement":"CaCl2 increased aspartame hydrolysis 2.9–4.5-fold in the tested human and pig membrane preparations.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"d982d5c5-cad2-5b65-921f-7d28a09e397e","mechanism_event_label":"Calcium availability affected measured enzyme activity.","subject":{"id":"e359bc15-e675-5d83-b0fe-1d70814e130b","slug":"calcium-ion","display_name":"Calcium ion","entity_type_key":"ion"},"object":{"id":"164d6ac3-0038-5071-b0dc-d6774ef52141","slug":"human-intestinal-aspartame-hydrolysis","display_name":"Human intestinal microvillar aspartame hydrolysis","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"d982d5c5-cad2-5b65-921f-7d28a09e397e","stable_key":"b428117d-3a8c-5e74-8711-168153e19545:aspartame-calcium-activation-event","event_type":"observed_relationship","label":"Calcium availability affected measured enzyme activity.","description":"CaCl2 increased aspartame hydrolysis 2.9–4.5-fold in the tested human and pig membrane preparations.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"e359bc15-e675-5d83-b0fe-1d70814e130b","slug":"calcium-ion","display_name":"Calcium ion","entity_type_key":"ion"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"164d6ac3-0038-5071-b0dc-d6774ef52141","slug":"human-intestinal-aspartame-hydrolysis","display_name":"Human intestinal microvillar aspartame hydrolysis","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"15bd1664-9d4b-514f-ab83-87274a67673f","slug":"aspartame","display_name":"Aspartame","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"6e964e46-4708-534b-a643-901f4372037b","slug":"human-enpep","display_name":"Human aminopeptidase A / ENPEP","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Ex vivo microvillar assays.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Not evidence that calcium supplements improve tolerance.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Aspartame collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"aspartame","display_name":"Aspartame","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Calcium availability affected measured enzyme activity.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Metabolism of aspartame by human and pig intestinal microvillar peptidases. · 1994 · https://pubmed.ncbi.nlm.nih.gov/8141778/ · DOI 10.1042/bj2980635","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"f3c00685-2f4c-5b86-8539-fd07dc1db7c9","evidence_kind":"source_excerpt","locator":"Lines 50-56","start_line":50,"end_line":56,"excerpt":"## aspartame-calcium-activation\nCalcium availability affected measured enzyme activity.\nCaCl2 increased aspartame hydrolysis 2.9–4.5-fold in the tested human and pig membrane preparations.\nModel: Ex vivo microvillar assays.\nLimitations: Not evidence that calcium supplements improve tolerance.\nEvidence access: Primary abstract\nMetabolism of aspartame by human and pig intestinal microvillar peptidases. · 1994 · https://pubmed.ncbi.nlm.nih.gov/8141778/ · DOI 10.1042/bj2980635","model_system":"Ex vivo microvillar assays.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"2bba7d36-993f-5ba7-acaf-2e4d573527ed","stable_key":"import-b428117d-3a8c-5e74-8711-168153e19545","title":"Aspartame: digestion, taste, metabolite dependencies and experimental signaling (2026-09-20)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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