{"id":"d3dc581a-1b5f-53df-ba33-bf5617974342","stable_key":"1310afbd-6010-586e-805d-551d846da421:b6-transport-enterocyte-pl-translocation","predicate":"undergoes","statement":"During apical PL incubation, basolateral PL appearance approximately matched apical disappearance, without detectable medium PLP or PMP.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"2518416c-b4ff-5982-a3e7-cf0572fef57a","mechanism_event_label":"Pyridoxal crossed the cell layer largely as pyridoxal.","subject":{"id":"9bf6269d-9e01-553d-bd8b-11a0d613bfca","slug":"pyridoxal","display_name":"Pyridoxal","entity_type_key":"small_molecule"},"object":{"id":"2c8fcd3f-43a4-5083-837f-0651c4abc331","slug":"intestinal-pyridoxal-translocation","display_name":"Intestinal pyridoxal translocation","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"2518416c-b4ff-5982-a3e7-cf0572fef57a","stable_key":"1310afbd-6010-586e-805d-551d846da421:b6-transport-enterocyte-pl-translocation-event","event_type":"biochemical_relationship","label":"Pyridoxal crossed the cell layer largely as pyridoxal.","description":"During apical PL incubation, basolateral PL appearance approximately matched apical disappearance, without detectable medium PLP or PMP.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"9bf6269d-9e01-553d-bd8b-11a0d613bfca","slug":"pyridoxal","display_name":"Pyridoxal","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"2c8fcd3f-43a4-5083-837f-0651c4abc331","slug":"intestinal-pyridoxal-translocation","display_name":"Intestinal pyridoxal translocation","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_location","value_text":"Results: Incubation with pyridoxal; Table 1","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Polarized human Caco-2 monolayers; human intestinal lysates.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"100 or 1000 nM PL; 6-48 h.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Cell monolayer model; PL and PN transport mechanisms need not be identical.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin B6 research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-b6","display_name":"Vitamin B6","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Pyridoxal crossed the cell layer largely as pyridoxal.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[albersen2013] The intestine plays a substantial role in human vitamin B6 metabolism: a Caco-2 cell model. (2013). https://pubmed.ncbi.nlm.nih.gov/23342087/ DOI: 10.1371/journal.pone.0054113","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Polarized Caco-2 monolayers","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"69b3ede2-ebd4-5fa3-9cea-7acc308cb557","evidence_kind":"source_excerpt","locator":"Lines 150-161","start_line":150,"end_line":161,"excerpt":"### b6-transport-enterocyte-pl-translocation\nDuring apical PL incubation, basolateral PL appearance approximately matched apical disappearance, without detectable medium PLP or PMP.\nCondition category: normal\nnutrient_topic: Vitamin B6 research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Pyridoxal crossed the cell layer largely as pyridoxal.\norganism: Homo sapiens\ntissue_or_cell_type: Polarized Caco-2 monolayers\nexperimental_model: Polarized human Caco-2 monolayers; human intestinal lysates.\nlimitations: Cell monolayer model; PL and PN transport mechanisms need not be identical.\nexposure: 100 or 1000 nM PL; 6-48 h.\nevidence_location: Results: Incubation with pyridoxal; Table 1\n[albersen2013] The intestine plays a substantial role in human vitamin B6 metabolism: a Caco-2 cell model. (2013). https://pubmed.ncbi.nlm.nih.gov/23342087/ DOI: 10.1371/journal.pone.0054113","model_system":"Polarized human Caco-2 monolayers; human intestinal lysates.","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [albersen2013] The intestine plays a substantial role in human vitamin B6 metabolism: a Caco-2 cell model. (2013). https://pubmed.ncbi.nlm.nih.gov/23342087/ DOI: 10.1371/journal.pone.0054113","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"251773bb-16f5-5903-b135-db4a61d9dec4","stable_key":"import-1310afbd-6010-586e-805d-551d846da421","title":"Vitamin B6: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. 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