{"id":"d3c5bae0-6384-5e5f-8ccc-9a45a3625d6e","stable_key":"c836a883-ac18-5eb2-9971-2f0b542feba8:allicin-formation","predicate":"reported_relationship","statement":"Two allylsulfenic-acid molecules condense to allicin with water loss.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"c0a4c92e-e6ba-5f5a-9157-768d01be7ef4","mechanism_event_label":"Two allylsulfenic-acid molecules condense to allicin with water loss.","subject":{"id":"21996d1e-416b-5f79-8938-86d94e38257f","slug":"allylsulfenic-acid","display_name":"Allylsulfenic acid","entity_type_key":"small_molecule"},"object":{"id":"85c86fcf-3060-5fae-b567-4f089990ab2d","slug":"allicin","display_name":"Allicin","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"c0a4c92e-e6ba-5f5a-9157-768d01be7ef4","stable_key":"c836a883-ac18-5eb2-9971-2f0b542feba8:allicin-formation-event","event_type":"biochemical_relationship","label":"Two allylsulfenic-acid molecules condense to allicin with water loss.","description":"**Alliinase and vitamin B6.** Garlic alliinase is a plant pyridoxal-5′-phosphate (PLP)-dependent enzyme, not a human enzyme. Structural studies resolved its cofactor-containing active site, an apo form, and a PLP-bound aminoacrylate intermediate. Alliin cleavage produces allylsulfenic acid and an aminoacrylate intermediate; the latter yields pyruvate and ammonia. Two allylsulfenic acid molecules condense to allicin with water loss. This links allicin formation to B6 chemistry in the plant. It does not show that taking B6 increases allicin production in a person. [Kuettner 2002](https://pubmed.ncbi.nlm.nih.gov/12235163/) [Shimon 2007](https://pubmed.ncbi.nlm.nih.gov/17174334/)","status":"provisional","compartment":null,"participants":[{"entity":{"id":"21996d1e-416b-5f79-8938-86d94e38257f","slug":"allylsulfenic-acid","display_name":"Allylsulfenic acid","entity_type_key":"small_molecule"},"role":"tested factor","stoichiometry":null,"state_label":"as reported","sequence_order":0,"notes":""},{"entity":{"id":"85c86fcf-3060-5fae-b567-4f089990ab2d","slug":"allicin","display_name":"Allicin","entity_type_key":"small_molecule"},"role":"measured outcome","stoichiometry":null,"state_label":"not_reported","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary indexed abstract reviewed; full methods, exact concentrations or species-specific attribution remain unextracted unless explicitly stated in the abstract or separately verified publisher text.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Garlic sulfur chemistry; spontaneous condensation follows the enzymatic cleavage.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Source-derived extraction of a fact-checked reference; access is explicit, not independent raw-data verification.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Interpret only within the recorded preparation, exposure and comparator. The complete source passage retains qualifications; unspecified doses/timing have not been extracted here. No clinical efficacy, nutrient deficiency or unique molecular mediation is inferred.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Two allylsulfenic-acid molecules condense to allicin with water loss.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Two structures of alliinase from Alliium sativum L.: apo form and ternary complex with aminoacrylate reaction intermediate covalently bound to the PLP cofactor. | 2007 | DOI 10.1016/j.jmb.2006.11.041 | PMID 17174334 | https://pubmed.ncbi.nlm.nih.gov/17174334/ | https://doi.org/10.1016/j.jmb.2006.11.041","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference lines 11-11; exact primary location described in quoted passage where extracted.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"32767718-d4ab-5b66-8d32-e258d5af017c","evidence_kind":"source_excerpt","locator":"Lines 11-11","start_line":11,"end_line":11,"excerpt":"**Alliinase and vitamin B6.** Garlic alliinase is a plant pyridoxal-5′-phosphate (PLP)-dependent enzyme, not a human enzyme. Structural studies resolved its cofactor-containing active site, an apo form, and a PLP-bound aminoacrylate intermediate. Alliin cleavage produces allylsulfenic acid and an aminoacrylate intermediate; the latter yields pyruvate and ammonia. Two allylsulfenic acid molecules condense to allicin with water loss. This links allicin formation to B6 chemistry in the plant. It does not show that taking B6 increases allicin production in a person. [Kuettner 2002](https://pubmed.ncbi.nlm.nih.gov/12235163/) [Shimon 2007](https://pubmed.ncbi.nlm.nih.gov/17174334/)","model_system":"Garlic sulfur chemistry; spontaneous condensation follows the enzymatic cleavage.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact excerpt of the retained AI-assisted reviewed reference; primary sources are cited in primary_references and access scope is retained. Not a verbatim quotation from a primary paper.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"10820a6b-594a-547d-97f9-906ab4cc1d6e","stable_key":"import-c836a883-ac18-5eb2-9971-2f0b542feba8","title":"Allicin: detailed mechanisms of action (reviewed 5 October 2026)","document_type":"imported_text","citation_label":"Original AI-assisted review of primary studies and, where relevant, official regulatory records. Access level is retained per claim. Corrections, null results and unresolved questions remain explicit. Not publisher full text or independent replication.","file_path":"","sha256":"2475d3eb681100a0a34577a47b7cba5b251df866fbd6ce795122ccabee360018","revision_id":"590df96d-2ed1-5763-b04c-bf0e096e603c","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}