{"id":"d3ab000e-9bd5-5f35-8409-6c43a8108c37","stable_key":"584c58f5-ab9f-53f3-97a9-55783db943b0:tryptophan-ipa-mouse-pxr","predicate":"activates","statement":"Indolepropionate activated mouse PXR with an EC50 near 0.55 micromolar in the reporter assay.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"bf39f958-1047-5279-bdfa-55569ba98534","mechanism_event_label":"The mouse receptor was sensitive to this microbial product.","subject":{"id":"813fc8bf-f15a-5201-91e3-d1579084376d","slug":"indole-3-propionic-acid","display_name":"Indole-3-propionic acid / indolepropionate","entity_type_key":"small_molecule"},"object":{"id":"10e4b201-804c-52c7-985a-1a7047f0cdc5","slug":"mouse-nr1i2","display_name":"Mouse pregnane X receptor / Pxr / Nr1i2","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"bf39f958-1047-5279-bdfa-55569ba98534","stable_key":"584c58f5-ab9f-53f3-97a9-55783db943b0:tryptophan-ipa-mouse-pxr-event","event_type":"observed_relationship","label":"The mouse receptor was sensitive to this microbial product.","description":"Indolepropionate activated mouse PXR with an EC50 near 0.55 micromolar in the reporter assay.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"813fc8bf-f15a-5201-91e3-d1579084376d","slug":"indole-3-propionic-acid","display_name":"Indole-3-propionic acid / indolepropionate","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"10e4b201-804c-52c7-985a-1a7047f0cdc5","slug":"mouse-nr1i2","display_name":"Mouse pregnane X receptor / Pxr / Nr1i2","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"769339cb-213b-559e-acc0-07ed00368b94","slug":"l-tryptophan","display_name":"L-Tryptophan","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text, Figure 1","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mouse PXR expressed in 293T cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Expression host does not make the receptor human; potency differed sharply for human PXR.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Tryptophan collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-tryptophan","display_name":"L-Tryptophan","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The mouse receptor was sensitive to this microbial product.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Symbiotic bacterial metabolites regulate gastrointestinal barrier function via the xenobiotic sensor PXR and Toll-like receptor 4. · 2014 · https://pubmed.ncbi.nlm.nih.gov/25065623/ · DOI 10.1016/j.immuni.2014.06.014","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"6a1c4a13-e775-51a9-8e73-f6d709d55c93","evidence_kind":"source_excerpt","locator":"Lines 522-528","start_line":522,"end_line":528,"excerpt":"## tryptophan-ipa-mouse-pxr\nThe mouse receptor was sensitive to this microbial product.\nIndolepropionate activated mouse PXR with an EC50 near 0.55 micromolar in the reporter assay.\nModel: Mouse PXR expressed in 293T cells.\nLimitations: Expression host does not make the receptor human; potency differed sharply for human PXR.\nEvidence access: Primary full text, Figure 1\nSymbiotic bacterial metabolites regulate gastrointestinal barrier function via the xenobiotic sensor PXR and Toll-like receptor 4. · 2014 · https://pubmed.ncbi.nlm.nih.gov/25065623/ · DOI 10.1016/j.immuni.2014.06.014","model_system":"Mouse PXR expressed in 293T cells.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"73f9d3e7-fdc9-5418-8f3c-f4ef145f6efa","stable_key":"import-584c58f5-ab9f-53f3-97a9-55783db943b0","title":"Tryptophan: transport, protein synthesis, neuroactive metabolites, NAD and microbial pathways (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"7dfd20063b91900cb5c6ad675e3e675b7e25ef0fb8304eabac6f263cc5518284","revision_id":"bfafd789-fdf5-521f-bd28-c662e01ec1ea","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}