{"id":"d344dab8-fbec-5758-a341-696560fab537","stable_key":"ee4227e5-80d7-55e9-ada7-3c5a16f93ac9:cla-txb2","predicate":"reduces","statement":"The mixture and individual isomers reduced arachidonate-derived TXB2 formation, while platelet 12-HETE formation was not inhibited.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"8ec9faf0-03b1-507c-8430-00210a7bf4f4","mechanism_event_label":"Two branches from the same fatty acid responded differently.","subject":{"id":"6f0cf7bd-c6e5-52d1-92f8-dd5c879e3c57","slug":"cla-mixed-isomer-preparations","display_name":"Study-specific mixed-isomer CLA preparations","entity_type_key":"chemical_species"},"object":{"id":"927edea8-ee1d-5697-a3bd-c8f020af2364","slug":"human-platelet-txb2-output","display_name":"Human platelet thromboxane B2 formation","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"8ec9faf0-03b1-507c-8430-00210a7bf4f4","stable_key":"ee4227e5-80d7-55e9-ada7-3c5a16f93ac9:cla-txb2-event","event_type":"observed_relationship","label":"Two branches from the same fatty acid responded differently.","description":"The mixture and individual isomers reduced arachidonate-derived TXB2 formation, while platelet 12-HETE formation was not inhibited.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"6f0cf7bd-c6e5-52d1-92f8-dd5c879e3c57","slug":"cla-mixed-isomer-preparations","display_name":"Study-specific mixed-isomer CLA preparations","entity_type_key":"chemical_species"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"927edea8-ee1d-5697-a3bd-c8f020af2364","slug":"human-platelet-txb2-output","display_name":"Human platelet thromboxane B2 formation","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"39f9446f-52a6-502d-b507-91137726f82c","slug":"arachidonic-acid","display_name":"AA","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"3b200ef2-893f-5ca7-93f4-ea657577a760","slug":"thromboxane-b2","display_name":"Thromboxane B2","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"bb3af9eb-b30f-551e-8c8a-c5278e50da3e","slug":"12-hete","display_name":"12-Hydroxyeicosatetraenoic acid / 12-HETE","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"a85c068b-9c0b-5a08-bad7-66ff3ef2af19","slug":"cla-cis9-trans11","display_name":"cis-9,trans-11 conjugated linoleic acid / rumenic acid","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""},{"entity":{"id":"4405f918-c17d-5365-b873-1678989f8ac1","slug":"cla-trans10-cis12","display_name":"trans-10,cis-12 conjugated linoleic acid","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":6,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human platelet radiotracer assay.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"TXB2 is the measured readout of TXA2 formation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"CLA collection; isomer, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"conjugated-linoleic-acid","display_name":"Conjugated linoleic acid / CLA isomer family","entity_type_key":"chemical_species"}},{"dimension":"plain_language","value_text":"Two branches from the same fatty acid responded differently.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Antiplatelet effects of conjugated linoleic acid isomers. · 1999 · https://pubmed.ncbi.nlm.nih.gov/10320806/ · DOI 10.1016/s1388-1981(99)00055-4","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"c6fa6a2b-5423-5cc6-8683-257dad85335e","evidence_kind":"source_excerpt","locator":"Lines 286-292","start_line":286,"end_line":292,"excerpt":"## cla-txb2\nTwo branches from the same fatty acid responded differently.\nThe mixture and individual isomers reduced arachidonate-derived TXB2 formation, while platelet 12-HETE formation was not inhibited.\nModel: Human platelet radiotracer assay.\nLimitations: TXB2 is the measured readout of TXA2 formation.\nEvidence access: Primary abstract\nAntiplatelet effects of conjugated linoleic acid isomers. · 1999 · https://pubmed.ncbi.nlm.nih.gov/10320806/ · DOI 10.1016/s1388-1981(99)00055-4","model_system":"Human platelet radiotracer assay.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; 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