{"id":"d2d92af4-a005-534c-a474-a1daf3984c48","stable_key":"8182578e-8d21-598e-a3cb-e62d480d2251:myricetin-ape1-magnesium","predicate":"required_in_assay_for","statement":"Omitting MgCl2 abolished detectable APE1 incision in the 2023 control reactions.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"c280a936-4152-57fd-b3b1-b9eabffff026","mechanism_event_label":"The repair enzyme also has a mineral-dependent assay requirement.","subject":{"id":"bff427ab-35f9-59c2-bb24-fd5953bbaec2","slug":"magnesium-ion","display_name":"Mg2+","entity_type_key":"ion"},"object":{"id":"4eef1de4-c3ff-555b-baaa-a48cf20def4f","slug":"apex1","display_name":"Human AP endonuclease 1 / APE1 / APEX1","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"c280a936-4152-57fd-b3b1-b9eabffff026","stable_key":"8182578e-8d21-598e-a3cb-e62d480d2251:myricetin-ape1-magnesium-event","event_type":"observed_relationship","label":"The repair enzyme also has a mineral-dependent assay requirement.","description":"Omitting MgCl2 abolished detectable APE1 incision in the 2023 control reactions.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"bff427ab-35f9-59c2-bb24-fd5953bbaec2","slug":"magnesium-ion","display_name":"Mg2+","entity_type_key":"ion"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"4eef1de4-c3ff-555b-baaa-a48cf20def4f","slug":"apex1","display_name":"Human AP endonuclease 1 / APE1 / APEX1","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"fafe05e7-4874-56e6-a42a-2ebf1ab2dd57","slug":"myricetin","display_name":"Myricetin","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human APE1 delta-N38; standard reaction initiated with 2 mM MgCl2.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Does not prove myricetin inhibits by magnesium chelation or causes magnesium deficiency.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Myricetin collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"myricetin","display_name":"Myricetin","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The repair enzyme also has a mineral-dependent assay requirement.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Characterizing inhibitors of human AP endonuclease 1. · 2023 · https://pubmed.ncbi.nlm.nih.gov/36652434/ · DOI 10.1371/journal.pone.0280526","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"d9f5d3eb-3197-5fdc-8594-79760e8da8e8","evidence_kind":"source_excerpt","locator":"Lines 676-682","start_line":676,"end_line":682,"excerpt":"## myricetin-ape1-magnesium\nThe repair enzyme also has a mineral-dependent assay requirement.\nOmitting MgCl2 abolished detectable APE1 incision in the 2023 control reactions.\nModel: Human APE1 delta-N38; standard reaction initiated with 2 mM MgCl2.\nLimitations: Does not prove myricetin inhibits by magnesium chelation or causes magnesium deficiency.\nEvidence access: Primary full text\nCharacterizing inhibitors of human AP endonuclease 1. · 2023 · https://pubmed.ncbi.nlm.nih.gov/36652434/ · DOI 10.1371/journal.pone.0280526","model_system":"Human APE1 delta-N38; standard reaction initiated with 2 mM MgCl2.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"58045cd9-3b27-5469-bb4b-e1c6a9bdca34","stable_key":"import-8182578e-8d21-598e-a3cb-e62d480d2251","title":"Myricetin: metabolism, immune signaling, redox chemistry and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"0abf512275cf3aae9e4ec8544f1cde97e0155d0cfb5be12a193cdd5a898386a7","revision_id":"422e342f-a55c-5e73-ba98-43df560f49b9","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}