{"id":"d27c6ab1-83c3-5ebc-a266-24b82c9b7cf7","stable_key":"10aa417f-4b03-599f-a453-4aa09edeb27c:alanine-ampk-carbon-rescue","predicate":"attenuates_alanine_induced","statement":"Pyruvate or TCA-intermediate supplementation restored ATP and attenuated alanine-induced AMPK phosphorylation in the tested liver-cell experiments.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"8c70bd62-7199-53a6-bd17-42b5e1836ad6","mechanism_event_label":"Providing usable carbon changed the signaling response to alanine.","subject":{"id":"91e6d4f5-fba1-542d-b68a-57cc28e1e425","slug":"pyruvate","display_name":"Pyruvate","entity_type_key":"small_molecule"},"object":{"id":"2f112afe-1ecd-54a0-a90d-a8c6ac5cecb6","slug":"rat-ampk-complex","display_name":"Rat AMP-activated protein kinase complex","entity_type_key":"protein_complex"},"evidence_count":1,"mechanism_event":{"id":"8c70bd62-7199-53a6-bd17-42b5e1836ad6","stable_key":"10aa417f-4b03-599f-a453-4aa09edeb27c:alanine-ampk-carbon-rescue-event","event_type":"observed_relationship","label":"Providing usable carbon changed the signaling response to alanine.","description":"Pyruvate or TCA-intermediate supplementation restored ATP and attenuated alanine-induced AMPK phosphorylation in the tested liver-cell experiments.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"91e6d4f5-fba1-542d-b68a-57cc28e1e425","slug":"pyruvate","display_name":"Pyruvate","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"2f112afe-1ecd-54a0-a90d-a8c6ac5cecb6","slug":"rat-ampk-complex","display_name":"Rat AMP-activated protein kinase complex","entity_type_key":"protein_complex"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"ebdc4461-c059-563e-88b0-422c21fdaa25","slug":"alanine","display_name":"L-Alanine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"58b974f1-d389-5bf6-81cd-889c44442c42","slug":"atp","display_name":"ATP","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"628ddc2d-e4d9-59c7-ae76-0c0cab80a6b1","slug":"2-oxoglutarate","display_name":"2-Oxoglutarate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Rat H4IIE nutrient-addition experiments.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"A biochemical rescue does not establish a supplement combination for humans.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Alanine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"alanine","display_name":"L-Alanine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Providing usable carbon changed the signaling response to alanine.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"l-Alanine activates hepatic AMP-activated protein kinase and modulates systemic glucose metabolism. · 2018 · https://pubmed.ncbi.nlm.nih.gov/30190193/ · DOI 10.1016/j.molmet.2018.08.002","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"4aa58566-1bb2-5d59-8e26-a049efb49500","evidence_kind":"source_excerpt","locator":"Lines 272-278","start_line":272,"end_line":278,"excerpt":"## alanine-ampk-carbon-rescue\nProviding usable carbon changed the signaling response to alanine.\nPyruvate or TCA-intermediate supplementation restored ATP and attenuated alanine-induced AMPK phosphorylation in the tested liver-cell experiments.\nModel: Rat H4IIE nutrient-addition experiments.\nLimitations: A biochemical rescue does not establish a supplement combination for humans.\nEvidence access: Primary full text\nl-Alanine activates hepatic AMP-activated protein kinase and modulates systemic glucose metabolism. · 2018 · https://pubmed.ncbi.nlm.nih.gov/30190193/ · DOI 10.1016/j.molmet.2018.08.002","model_system":"Rat H4IIE nutrient-addition experiments.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; 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