{"id":"d25fc556-1d33-54b4-b702-cac8f902b6b9","stable_key":"bf161ddf-8b82-5d65-b86d-0b41245aac61:phlorizin-intestinal-hydrolysis","predicate":"is_hydrolyzed_before_transport_to","statement":"In rat jejunum/ileum perfusion, phlorizin hydrolysis to aglycone preceded intestinal conjugation and transport, and only conjugated forms entered mesenteric blood.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"e45f4f70-3e23-5aba-a108-f0a99eda475b","mechanism_event_label":"In rat jejunum/ileum perfusion, phlorizin hydrolysis to aglycone preceded intestinal conjugation and transport, and only conjugated forms entered mesenteric blood.","subject":{"id":"3516a0ba-1f72-5e1b-bccb-7fca992f96f7","slug":"phlorizin","display_name":"Phlorizin","entity_type_key":"small_molecule"},"object":{"id":"83949207-904f-53dc-bc9d-f7afaa546b34","slug":"phloretin","display_name":"Phloretin","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"e45f4f70-3e23-5aba-a108-f0a99eda475b","stable_key":"bf161ddf-8b82-5d65-b86d-0b41245aac61:phlorizin-intestinal-hydrolysis-event","event_type":"observed_relationship","label":"In rat jejunum/ileum perfusion, phlorizin hydrolysis to aglycone preceded intestinal conjugation and transport, and only conjugated forms entered mesenteric blood.","description":"In rat jejunum/ileum perfusion, phlorizin hydrolysis to aglycone preceded intestinal conjugation and transport, and only conjugated forms entered mesenteric blood.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"3516a0ba-1f72-5e1b-bccb-7fca992f96f7","slug":"phlorizin","display_name":"Phlorizin","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"83949207-904f-53dc-bc9d-f7afaa546b34","slug":"phloretin","display_name":"Phloretin","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"8927515b-b76d-5d9f-9eb4-f967f0b77804","slug":"lactase-phlorizin-hydrolase","display_name":"Lactase-phlorizin hydrolase / LCT","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"dose","value_text":"Phlorizin or phloretin 15 nmol/min for 30 minutes","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"30 minutes","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary PubMed abstract and indexed metadata reviewed. 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(2001). https://pubmed.ncbi.nlm.nih.gov/11481403/ DOI: 10.1093/jn/131.8.2109","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"Intestinal perfusion","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"Luminal, mucosal and mesenteric-blood metabolites","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"594b4164-db8f-5592-b143-d253c803480a","evidence_kind":"source_excerpt","locator":"Lines 44-53","start_line":44,"end_line":53,"excerpt":"## phlorizin-intestinal-hydrolysis\nIn rat jejunum/ileum perfusion, phlorizin hydrolysis to aglycone preceded intestinal conjugation and transport, and only conjugated forms entered mesenteric blood.\nModel/species: In-situ rat jejunum plus ileum perfusion\nTissue/system: Luminal, mucosal and mesenteric-blood metabolites\nExposure: Phlorizin or phloretin 15 nmol/min for 30 minutes\nRoute: Intestinal perfusion\nDuration: 30 minutes\nLimits: Rat intestinal handling may not quantify human exposure; conjugate identities and free fractions determine downstream action.\nPrimary reference: Comparison of the intestinal absorption of quercetin, phloretin and their glucosides in rats. (2001). https://pubmed.ncbi.nlm.nih.gov/11481403/ DOI: 10.1093/jn/131.8.2109\nAccess: Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","model_system":"In-situ rat jejunum plus ileum perfusion","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact original curation span with primary citation; not a publisher quotation.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"7d4bfcb9-1665-5686-8b8a-1ad17ad6a4d2","stable_key":"import-bf161ddf-8b82-5d65-b86d-0b41245aac61","title":"Phlorizin: mechanism of action and interactions (2026-09-20)","document_type":"imported_text","citation_label":"Original AI-assisted source-specific curation with primary-study citations, model, exposure, route, duration, negative findings and limitations preserved. 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