{"id":"d24babc6-8197-5122-96e2-24068f625468","stable_key":"ec987f3e-0b3c-55cc-b304-c703a738e35d:egcg-comt-metabolite","predicate":"inhibits","statement":"4″-O-methyl-EGCG retained COMT inhibitory activity, IC50 0.10 micromolar.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"95112c74-e1da-5209-9ffa-eca08476c710","mechanism_event_label":"A methylated metabolite also affected the enzyme in vitro.","subject":{"id":"2a8ad3ba-ad17-56f8-9a40-02f941c1d17f","slug":"egcg-4-double-prime-o-methyl","display_name":"4″-O-methyl-EGCG","entity_type_key":"small_molecule"},"object":{"id":"936e1fd4-e862-5e32-b7dc-35b946294e43","slug":"comt","display_name":"Human catechol O-methyltransferase / COMT","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"95112c74-e1da-5209-9ffa-eca08476c710","stable_key":"ec987f3e-0b3c-55cc-b304-c703a738e35d:egcg-comt-metabolite-event","event_type":"observed_relationship","label":"A methylated metabolite also affected the enzyme in vitro.","description":"4″-O-methyl-EGCG retained COMT inhibitory activity, IC50 0.10 micromolar.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"2a8ad3ba-ad17-56f8-9a40-02f941c1d17f","slug":"egcg-4-double-prime-o-methyl","display_name":"4″-O-methyl-EGCG","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"936e1fd4-e862-5e32-b7dc-35b946294e43","slug":"comt","display_name":"Human catechol O-methyltransferase / COMT","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"experimental_model","value_text":"Human liver cytosol; catechol-estrogen assay.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Do not assume metabolites have identical tissue exposure or activity to EGCG.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"EGCG collection; comparator and shared-pathway records retain their actual intervention.","comparator":null,"unit":null,"notes":"","entity":{"slug":"egcg","display_name":"Epigallocatechin-3-gallate (EGCG)","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A methylated metabolite also affected the enzyme in vitro.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Inhibition of human liver catechol-O-methyltransferase by tea catechins and their metabolites: structure-activity relationship and molecular-modeling studies. · 2005 · https://pubmed.ncbi.nlm.nih.gov/15857617/ · DOI 10.1016/j.bcp.2005.01.024","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"67229086-9e27-5a43-bf1b-e95d7a262775","evidence_kind":"source_excerpt","locator":"Lines 172-178","start_line":172,"end_line":178,"excerpt":"## egcg-comt-metabolite\nA methylated metabolite also affected the enzyme in vitro.\n4″-O-methyl-EGCG retained COMT inhibitory activity, IC50 0.10 micromolar.\nModel: Human liver cytosol; catechol-estrogen assay.\nLimitations: Do not assume metabolites have identical tissue exposure or activity to EGCG.\nEvidence access: primary abstract.\nInhibition of human liver catechol-O-methyltransferase by tea catechins and their metabolites: structure-activity relationship and molecular-modeling studies. · 2005 · https://pubmed.ncbi.nlm.nih.gov/15857617/ · DOI 10.1016/j.bcp.2005.01.024","model_system":"Human liver cytosol; catechol-estrogen assay.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Primary-abstract paraphrase; no full-text methods verification claimed.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"1f6d09e8-3aae-5c41-9ea7-15d0d1cab72d","stable_key":"import-ec987f3e-0b3c-55cc-b304-c703a738e35d","title":"EGCG: receptor signaling, metabolism, nutrient interactions and discovery questions (2026-09-18)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"b101c8d4d96ee03ff97eb1922c7bb0f96943295af473fdc3ef98b0dbd62fe8c7","revision_id":"e19bc5b2-ee3c-5f67-b5c5-9dc3b7d5f850","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}