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product identified by HPLC and tandem mass spectrometry.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Host-cell species is not the enzyme species; no dietary brain-delivery effect was tested.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Aspartate collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-aspartate","display_name":"L-Aspartate","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Aspartate-derived NAA can feed a second, glutamate-containing product.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Molecular characterization of N-acetylaspartylglutamate synthetase. · 2010 · https://pubmed.ncbi.nlm.nih.gov/20643647/ · DOI 10.1074/jbc.M110.111765","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"32671fc2-78e8-5d5d-980e-c1fab1c6b0fa","evidence_kind":"source_excerpt","locator":"Lines 370-376","start_line":370,"end_line":376,"excerpt":"## l-aspartate-naa-naag-supply\nAspartate-derived NAA can feed a second, glutamate-containing product.\nCoexpressing mouse Nat8l and Rimklb in CHO-K1 or HEK293T cells enabled NAAG production from the NAA-synthesis pathway.\nModel: Mouse-brain cDNA constructs in hamster/human cell hosts; product identified by HPLC and tandem mass spectrometry.\nLimitations: Host-cell species is not the enzyme species; no dietary brain-delivery effect was tested.\nEvidence access: Primary abstract and primary methods/figure text\nMolecular characterization of N-acetylaspartylglutamate synthetase. · 2010 · https://pubmed.ncbi.nlm.nih.gov/20643647/ · DOI 10.1074/jbc.M110.111765","model_system":"Mouse-brain cDNA constructs in hamster/human cell hosts; product identified by HPLC and tandem mass spectrometry.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"67970dcb-34e0-5b0a-8c86-d8c3cc183444","stable_key":"import-e37461ea-ea5d-5e2c-8091-138305f6dd70","title":"L-Aspartate: redox transfer, nitrogen partitioning and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; 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