{"id":"d2445842-4a12-5974-8577-b78edb0df69d","stable_key":"1deb434a-3547-51a0-a038-87b1ce79ec38:vd-act-cd36-uptake","predicate":"increases_uptake","statement":"Expression of CD36 in HEK cells increased cholecalciferol uptake, and its corresponding inhibitor reduced this uptake.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"21851404-3d11-556e-8db0-5f8248f5d151","mechanism_event_label":"CD36 can contribute to vitamin D3 entry into cells.","subject":{"id":"14bc9a7c-abcb-5d72-a113-2cf139a52474","slug":"cd36","display_name":"Human CD36 scavenger receptor","entity_type_key":"protein"},"object":{"id":"07f07b97-3fa1-5633-95b9-8f4cc0c11a23","slug":"intestinal-cholecalciferol-uptake","display_name":"Intestinal cholecalciferol uptake","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"21851404-3d11-556e-8db0-5f8248f5d151","stable_key":"1deb434a-3547-51a0-a038-87b1ce79ec38:vd-act-cd36-uptake-event","event_type":"biochemical_relationship","label":"CD36 can contribute to vitamin D3 entry into cells.","description":"Expression of CD36 in HEK cells increased cholecalciferol uptake, and its corresponding inhibitor reduced this uptake.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"14bc9a7c-abcb-5d72-a113-2cf139a52474","slug":"cd36","display_name":"Human CD36 scavenger receptor","entity_type_key":"protein"},"role":"transporter","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"b162e431-b442-5400-9335-ec91f7875a4e","slug":"cholecalciferol","display_name":"Cholecalciferol","entity_type_key":"small_molecule"},"role":"cargo","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"07f07b97-3fa1-5633-95b9-8f4cc0c11a23","slug":"intestinal-cholecalciferol-uptake","display_name":"Intestinal cholecalciferol uptake","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"false","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_location","value_text":"Primary abstract, Methods and results; exact incubation concentrations/durations unavailable in abstract.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Transfected HEK-cell uptake assay; intestinal relevance tested separately","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Transporter transfection and selective-inhibitor co-incubation; exact dose/time absent from abstract.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"HEK overexpression is not intact human intestine; inhibitors and uptake assays do not establish clinical deficiency. In-vivo ezetimibe effect in mice was nonsignificant.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient","value_text":"Vitamin D2 and D3","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-d","display_name":"Vitamin D2 and D3","entity_type_key":"chemical_species"}},{"dimension":"nutrient_topic","value_text":"Vitamin D2 and D3 research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-d","display_name":"Vitamin D2 and D3","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"CD36 can contribute to vitamin D3 entry into cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[reboul2011] Vitamin D intestinal absorption is not a simple passive diffusion: evidences for involvement of cholesterol transporters. (2011). https://pubmed.ncbi.nlm.nih.gov/21280209/ DOI: 10.1002/mnfr.201000553","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"HEK cell model of a candidate intestinal uptake mechanism","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"faa8f1d9-58cd-5d61-aacf-943deb80ab49","evidence_kind":"source_excerpt","locator":"Lines 181-194","start_line":181,"end_line":194,"excerpt":"### vd-act-cd36-uptake\nExpression of CD36 in HEK cells increased cholecalciferol uptake, and its corresponding inhibitor reduced this uptake.\nCondition category: normal\nnutrient_topic: Vitamin D2 and D3 research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: CD36 can contribute to vitamin D3 entry into cells.\norganism: Homo sapiens\ntissue_or_cell_type: HEK cell model of a candidate intestinal uptake mechanism\nexperimental_model: Transfected HEK-cell uptake assay; intestinal relevance tested separately\nlimitations: HEK overexpression is not intact human intestine; inhibitors and uptake assays do not establish clinical deficiency. In-vivo ezetimibe effect in mice was nonsignificant.\nexposure: Transporter transfection and selective-inhibitor co-incubation; exact dose/time absent from abstract.\ncross_nutrient: false\nevidence_location: Primary abstract, Methods and results; exact incubation concentrations/durations unavailable in abstract.\nnutrient: Vitamin D2 and D3\n[reboul2011] Vitamin D intestinal absorption is not a simple passive diffusion: evidences for involvement of cholesterol transporters. (2011). https://pubmed.ncbi.nlm.nih.gov/21280209/ DOI: 10.1002/mnfr.201000553","model_system":"Transfected HEK-cell uptake assay; intestinal relevance tested separately","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [reboul2011] Vitamin D intestinal absorption is not a simple passive diffusion: evidences for involvement of cholesterol transporters. 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