{"id":"d1fe8d0d-36f7-582a-b148-bb6c82d04918","stable_key":"3b5aff9b-4086-5574-bfb4-3ea49ba520d7:coq10-coq7-nadh","predicate":"uses","statement":"COQ7 hydroxylation assays consumed NADH in a substrate-dependent manner.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"31b7342f-3c07-5037-8d81-297f04c29de2","mechanism_event_label":"Niacin-derived reducing power also feeds a later synthesis step.","subject":{"id":"54ac8659-e9cf-50cc-a863-9aeabddbb7fe","slug":"tet-coq7","display_name":"Reconstructed ancestral tetrapod COQ7","entity_type_key":"protein"},"object":{"id":"9fbf2e03-16a9-5ca7-965c-0bfc07ca24da","slug":"nadh","display_name":"NADH","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"31b7342f-3c07-5037-8d81-297f04c29de2","stable_key":"3b5aff9b-4086-5574-bfb4-3ea49ba520d7:coq10-coq7-nadh-event","event_type":"biochemical_relationship","label":"Niacin-derived reducing power also feeds a later synthesis step.","description":"COQ7 hydroxylation assays consumed NADH in a substrate-dependent manner.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"0f973474-2a1d-52d9-a9a8-05e06762c4a3","slug":"diiron-active-site","display_name":"Diiron catalytic center","entity_type_key":"chemical_species"},"role":"catalytic_center","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"a59dbc3e-78d4-514c-a725-2536329a94d4","slug":"coq-c6-hydroxylation","display_name":"Coenzyme Q precursor C6 hydroxylation","entity_type_key":"cellular_process"},"role":"reaction","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"54ac8659-e9cf-50cc-a863-9aeabddbb7fe","slug":"tet-coq7","display_name":"Reconstructed ancestral tetrapod COQ7","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"9fbf2e03-16a9-5ca7-965c-0bfc07ca24da","slug":"nadh","display_name":"NADH","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/coq10-research/38425362.fulltext.txt\", \"locator\": \"Primary full-text span; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"7d25af4ecd7340649536b8fea3b8a0a308a1611bbf8c57f6a79b362f443f1cba\", \"start_char\": 23488, \"end_char\": 27391, \"text_sha256\": \"7147c9d517477cd7bbec4a155e5f13bc733d294380975977eba11b64517047f3\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Purified reconstructed COQ metabolon with short-chain substrates","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Enzyme combinations, methyl donors, reductants and metal additions","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Ancestral proteins and CoQ1 analogues; no clinical cofactor dose or proof of nutritional rate limitation. Reaction order need not be universal across species.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Coenzyme Q10 research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"coq10","display_name":"Coenzyme Q10 / CoQ10 redox system","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Reconstructed ancestral tetrapod proteins","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Niacin-derived reducing power also feeds a later synthesis step.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[coq10-p38425362] In vitro construction of the COQ metabolon unveils the molecular determinants of coenzyme Q biosynthesis. (2024). https://pubmed.ncbi.nlm.nih.gov/38425362/ DOI: 10.1038/s41929-023-01087-z","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Stepwise CoQ head-group assembly","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"b1279e89-b5d4-542b-9006-d5aadb6a5bcb","evidence_kind":"source_excerpt","locator":"Lines 723-734","start_line":723,"end_line":734,"excerpt":"### coq10-coq7-nadh\nCOQ7 hydroxylation assays consumed NADH in a substrate-dependent manner.\nCondition category: normal\nnutrient_topic: Coenzyme Q10 research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Niacin-derived reducing power also feeds a later synthesis step.\norganism: Reconstructed ancestral tetrapod proteins\ntissue_or_cell_type: Stepwise CoQ head-group assembly\nexperimental_model: Purified reconstructed COQ metabolon with short-chain substrates\nlimitations: Ancestral proteins and CoQ1 analogues; no clinical cofactor dose or proof of nutritional rate limitation. Reaction order need not be universal across species.\nexposure: Enzyme combinations, methyl donors, reductants and metal additions\nevidence_span: {\"source_cache\": \"artifacts/coq10-research/38425362.fulltext.txt\", \"locator\": \"Primary full-text span; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"7d25af4ecd7340649536b8fea3b8a0a308a1611bbf8c57f6a79b362f443f1cba\", \"start_char\": 23488, \"end_char\": 27391, \"text_sha256\": \"7147c9d517477cd7bbec4a155e5f13bc733d294380975977eba11b64517047f3\"}\n[coq10-p38425362] In vitro construction of the COQ metabolon unveils the molecular determinants of coenzyme Q biosynthesis. (2024). https://pubmed.ncbi.nlm.nih.gov/38425362/ DOI: 10.1038/s41929-023-01087-z","model_system":"Purified reconstructed COQ metabolon with short-chain substrates","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [coq10-p38425362] In vitro construction of the COQ metabolon unveils the molecular determinants of coenzyme Q biosynthesis. (2024). https://pubmed.ncbi.nlm.nih.gov/38425362/ DOI: 10.1038/s41929-023-01087-z","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"fc6c2f3b-14a3-59de-848e-ca1c02fee1df","stable_key":"import-3b5aff9b-4086-5574-bfb4-3ea49ba520d7","title":"Coenzyme Q10: biosynthesis, electron transfer, antioxidant recycling and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"053f9a6f6c17321fa9fd271486d7f20a86a49b237424108de7dc8663372e73bf","revision_id":"028900ce-8ca4-5d13-8c86-6e327c071e64","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}