{"id":"d11eedcb-89aa-5099-818f-cf124aaab82b","stable_key":"c3df3634-4c3a-5099-a5d9-e4f6344c1084:shmt2-html-cleavage","predicate":"converted_to","statement":"Purified human SHMT2 also cleaves hydroxytrimethyllysine, with lower measured catalytic efficiency than SHMT1.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"5b3ccf16-06be-50c4-b63f-7615c99de6f4","mechanism_event_label":"SHMT2 provides another demonstrated aldolase activity.","subject":{"id":"30b9ea41-2d8d-58c7-8dfd-7307505f4576","slug":"hydroxytrimethyllysine","display_name":"(2S,3S)-3-Hydroxy-N-epsilon-trimethyllysine","entity_type_key":"small_molecule"},"object":{"id":"b11e7a9c-d4c0-5f9e-92d1-9f1ca432bab3","slug":"trimethylaminobutyraldehyde","display_name":"4-Trimethylaminobutyraldehyde","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"5b3ccf16-06be-50c4-b63f-7615c99de6f4","stable_key":"c3df3634-4c3a-5099-a5d9-e4f6344c1084:shmt2-html-cleavage-event","event_type":"biochemical_relationship","label":"SHMT2 provides another demonstrated aldolase activity.","description":"Purified human SHMT2 also cleaves hydroxytrimethyllysine, with lower measured catalytic efficiency than SHMT1.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"2559e210-62e5-5cd8-bc77-538a9aecd7c8","slug":"shmt2","display_name":"SHMT2","entity_type_key":"protein"},"role":"catalyst","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"30b9ea41-2d8d-58c7-8dfd-7307505f4576","slug":"hydroxytrimethyllysine","display_name":"(2S,3S)-3-Hydroxy-N-epsilon-trimethyllysine","entity_type_key":"small_molecule"},"role":"substrate","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"6b656ff5-9532-5da4-8eea-8ca163a48649","slug":"pyridoxal-phosphate","display_name":"PLP","entity_type_key":"small_molecule"},"role":"cofactor","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"b11e7a9c-d4c0-5f9e-92d1-9f1ca432bab3","slug":"trimethylaminobutyraldehyde","display_name":"4-Trimethylaminobutyraldehyde","entity_type_key":"small_molecule"},"role":"product","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"2b507258-430c-51fe-9fd2-e510c2c197a9","slug":"glycine","display_name":"Glycine","entity_type_key":"small_molecule"},"role":"product","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"experimental_model","value_text":"Purified human enzyme kinetic assay","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Mouse Tha1 specialization should not be assigned to a human THA1 enzyme.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"SHMT2 provides another demonstrated aldolase activity.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[osmes2024] One substrate many enzymes virtual screening uncovers missing genes of carnitine biosynthesis in human and mouse (2024). https://www.nature.com/articles/s41467-024-47466-3 DOI: 10.1038/s41467-024-47466-3","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Mitochondrial enzyme tested in vitro","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"b6e7095a-18b8-5788-8f68-3c25b3639b35","evidence_kind":"source_excerpt","locator":"Lines 187-195","start_line":187,"end_line":195,"excerpt":"### shmt2-html-cleavage\nPurified human SHMT2 also cleaves hydroxytrimethyllysine, with lower measured catalytic efficiency than SHMT1.\nPlain language: SHMT2 provides another demonstrated aldolase activity.\nCondition category: normal\norganism: Homo sapiens\ntissue_or_cell_type: Mitochondrial enzyme tested in vitro\nexperimental_model: Purified human enzyme kinetic assay\nlimitations: Mouse Tha1 specialization should not be assigned to a human THA1 enzyme.\n[osmes2024] One substrate many enzymes virtual screening uncovers missing genes of carnitine biosynthesis in human and mouse (2024). https://www.nature.com/articles/s41467-024-47466-3 DOI: 10.1038/s41467-024-47466-3","model_system":"Purified human enzyme kinetic assay","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact quote from the accompanying curation document, not from publisher text. Original study references: [osmes2024] One substrate many enzymes virtual screening uncovers missing genes of carnitine biosynthesis in human and mouse (2024). https://www.nature.com/articles/s41467-024-47466-3 DOI: 10.1038/s41467-024-47466-3","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"7633bde7-dcc9-5086-91c6-a45eb96857f3","stable_key":"import-c3df3634-4c3a-5099-a5d9-e4f6344c1084","title":"L-Lysine: mechanism-first literature curation (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"93998d47c21525409ba82f1c82ededf2893dff15fbe61c7deffc175b0e298e97","revision_id":"3897e31f-6624-59e1-a053-8a81b7361632","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}