{"id":"d1040233-3220-52c8-830b-6e2bd06244e1","stable_key":"e69ef6a0-0e23-5eac-aca1-cf333781b962:ino-ippk-zinc","predicate":"coordinates_with","statement":"The mouse Ippk structure identified a zinc-binding site involved in maintaining the enzyme’s structural organization.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"0e7b6140-a42d-5b31-835b-dc86afe63af1","mechanism_event_label":"Zinc is part of the architecture of this mouse inositol-phosphate enzyme; dietary benefit was not tested.","subject":{"id":"49c806c2-7041-5020-8b3b-fa04ffa122ac","slug":"zinc-ion","display_name":"Zinc(II) ion","entity_type_key":"ion"},"object":{"id":"cf925852-7b0f-5723-9b1a-33083d6fa4d4","slug":"mouse-ippk","display_name":"Mouse Ippk (inositol-pentakisphosphate 2-kinase)","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"0e7b6140-a42d-5b31-835b-dc86afe63af1","stable_key":"e69ef6a0-0e23-5eac-aca1-cf333781b962:ino-ippk-zinc-event","event_type":"biochemical_relationship","label":"Zinc is part of the architecture of this mouse inositol-phosphate enzyme; dietary benefit was not tested.","description":"The mouse Ippk structure identified a zinc-binding site involved in maintaining the enzyme’s structural organization.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"49c806c2-7041-5020-8b3b-fa04ffa122ac","slug":"zinc-ion","display_name":"Zinc(II) ion","entity_type_key":"ion"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"cf925852-7b0f-5723-9b1a-33083d6fa4d4","slug":"mouse-ippk","display_name":"Mouse Ippk (inositol-pentakisphosphate 2-kinase)","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/inositol-research/28450399.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"a7b18249458f30cd8f1147710856a6bf7f87ca1b045e97290a04ce7c7cbfbb78\", \"start_char\": 0, \"end_char\": 1555, \"text_sha256\": \"a7b18249458f30cd8f1147710856a6bf7f87ca1b045e97290a04ce7c7cbfbb78\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mouse enzyme crystallography and functional analysis","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Structure with substrates and zinc-site characterization","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Mouse protein; intracellular InsP6 synthesis is distinct from intestinal exposure to dietary phytate.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Inositol research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"inositol","display_name":"Inositol (stereoisomer family)","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Mus musculus protein","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Zinc is part of the architecture of this mouse inositol-phosphate enzyme; dietary benefit was not tested.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[ino-p28450399] The crystal structure of mammalian inositol 1,3,4,5,6-pentakisphosphate 2-kinase reveals a new zinc-binding site and key features for protein function. (2017). https://pubmed.ncbi.nlm.nih.gov/28450399/ DOI: 10.1074/jbc.m117.780395","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Purified Ippk","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"20dfd971-f86c-5df2-90b2-c63e7b181094","evidence_kind":"source_excerpt","locator":"Lines 756-767","start_line":756,"end_line":767,"excerpt":"### ino-ippk-zinc\nThe mouse Ippk structure identified a zinc-binding site involved in maintaining the enzyme’s structural organization.\nCondition category: normal\nnutrient_topic: Inositol research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Zinc is part of the architecture of this mouse inositol-phosphate enzyme; dietary benefit was not tested.\norganism: Mus musculus protein\ntissue_or_cell_type: Purified Ippk\nexperimental_model: Mouse enzyme crystallography and functional analysis\nlimitations: Mouse protein; intracellular InsP6 synthesis is distinct from intestinal exposure to dietary phytate.\nexposure: Structure with substrates and zinc-site characterization\nevidence_span: {\"source_cache\": \"artifacts/inositol-research/28450399.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"a7b18249458f30cd8f1147710856a6bf7f87ca1b045e97290a04ce7c7cbfbb78\", \"start_char\": 0, \"end_char\": 1555, \"text_sha256\": \"a7b18249458f30cd8f1147710856a6bf7f87ca1b045e97290a04ce7c7cbfbb78\"}\n[ino-p28450399] The crystal structure of mammalian inositol 1,3,4,5,6-pentakisphosphate 2-kinase reveals a new zinc-binding site and key features for protein function. (2017). https://pubmed.ncbi.nlm.nih.gov/28450399/ DOI: 10.1074/jbc.m117.780395","model_system":"Mouse enzyme crystallography and functional analysis","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [ino-p28450399] The crystal structure of mammalian inositol 1,3,4,5,6-pentakisphosphate 2-kinase reveals a new zinc-binding site and key features for protein function. (2017). https://pubmed.ncbi.nlm.nih.gov/28450399/ DOI: 10.1074/jbc.m117.780395","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"d590c659-b774-50e6-9f1d-aff61d5f7c9c","stable_key":"import-e69ef6a0-0e23-5eac-aca1-cf333781b962","title":"Inositol: synthesis, signaling, mineral interactions and conditional deficiency (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"f7adbcedad647d5f8a3cc9e46bfa515e83c508be9c2a4e9d59c5f5dfb0667d53","revision_id":"a82d187c-60fb-52d1-8482-eadb92860f33","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}