{"id":"d0f6d89d-6115-5c8c-950e-a95d05d9f1d2","stable_key":"5102beb6-9f61-500e-af10-9ac63649e0b7:renal-sgk1-phosphorylates-nedd4l","predicate":"phosphorylates","statement":"Sgk1 phosphorylated Nedd4-2 at Ser444 and less strongly Ser338 in Xenopus constructs.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"4446fa28-d22e-5e9a-afd8-08912deed351","mechanism_event_label":"SGK1 modifies the protein that normally restrains sodium channels.","subject":{"id":"4db8cea5-2f5d-5df0-881a-a725d1920e85","slug":"sgk1","display_name":"Serum and glucocorticoid-regulated kinase 1","entity_type_key":"protein"},"object":{"id":"11e2d9a3-7d3c-5b2c-938d-5a25b15d10d1","slug":"nedd4l","display_name":"NEDD4-like E3 ubiquitin protein ligase","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"4446fa28-d22e-5e9a-afd8-08912deed351","stable_key":"5102beb6-9f61-500e-af10-9ac63649e0b7:renal-sgk1-phosphorylates-nedd4l-event","event_type":"biochemical_relationship","label":"SGK1 modifies the protein that normally restrains sodium channels.","description":"Sgk1 phosphorylated Nedd4-2 at Ser444 and less strongly Ser338 in Xenopus constructs.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"4db8cea5-2f5d-5df0-881a-a725d1920e85","slug":"sgk1","display_name":"Serum and glucocorticoid-regulated kinase 1","entity_type_key":"protein"},"role":"causal-subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"11e2d9a3-7d3c-5b2c-938d-5a25b15d10d1","slug":"nedd4l","display_name":"NEDD4-like E3 ubiquitin protein ligase","entity_type_key":"protein"},"role":"measured-target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"58b974f1-d389-5bf6-81cd-889c44442c42","slug":"atp","display_name":"ATP","entity_type_key":"small_molecule"},"role":"phosphate-donor","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"e3d88ab0-725b-5cd0-9740-dee1509d4a1e","slug":"nedd4l-sgk-phosphorylated","display_name":"SGK-phosphorylated NEDD4L","entity_type_key":"protein_state"},"role":"product-state","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"b6e8049f-ad5c-5ca0-ba73-1d4392131397","slug":"enac","display_name":"Epithelial sodium channel","entity_type_key":"protein_complex"},"role":"downstream-channel","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"This biochemical sodium-channel pathway contributes to the machinery for K handling.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_location","value_text":"Figure 2.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Expression, kinase-dead and site-mutant experiments","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Residue numbers are construct/species-specific; nutritional K sensing was not tested.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Potassium research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"potassium","display_name":"Potassium","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Xenopus laevis","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"SGK1 modifies the protein that normally restrains sodium channels.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[debonneville-2001-sgk-nedd4] Phosphorylation of Nedd4-2 by Sgk1 regulates epithelial Na(+) channel cell surface expression (2001). https://pubmed.ncbi.nlm.nih.gov/11742982/ DOI: 10.1093/emboj/20.24.7052","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Oocytes","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"9edb5272-96e3-5d69-ad03-120dc083b6c1","evidence_kind":"source_excerpt","locator":"Lines 408-419","start_line":408,"end_line":419,"excerpt":"### renal-sgk1-phosphorylates-nedd4l\nSgk1 phosphorylated Nedd4-2 at Ser444 and less strongly Ser338 in Xenopus constructs.\nCondition category: normal\nnutrient_topic: Potassium research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: SGK1 modifies the protein that normally restrains sodium channels.\norganism: Xenopus laevis\ntissue_or_cell_type: Oocytes\nexperimental_model: Expression, kinase-dead and site-mutant experiments\nlimitations: Residue numbers are construct/species-specific; nutritional K sensing was not tested.\ncross_nutrient: This biochemical sodium-channel pathway contributes to the machinery for K handling.\nevidence_location: Figure 2.\n[debonneville-2001-sgk-nedd4] Phosphorylation of Nedd4-2 by Sgk1 regulates epithelial Na(+) channel cell surface expression (2001). https://pubmed.ncbi.nlm.nih.gov/11742982/ DOI: 10.1093/emboj/20.24.7052","model_system":"Expression, kinase-dead and site-mutant experiments","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [debonneville-2001-sgk-nedd4] Phosphorylation of Nedd4-2 by Sgk1 regulates epithelial Na(+) channel cell surface expression (2001). https://pubmed.ncbi.nlm.nih.gov/11742982/ DOI: 10.1093/emboj/20.24.7052","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"241226ee-6b7d-5cc7-9781-e6beb8ee1982","stable_key":"import-5102beb6-9f61-500e-af10-9ac63649e0b7","title":"Potassium: cross-nutrient mechanisms and deficiency (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"a8f89f8d84b48c0f93048dfc45f7f57a67dd9a7249968a096a14aeb03d8b7a52","revision_id":"de4ec6f4-58a1-537b-8169-97b783898df1","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}